Oleate uptake by isolated hepatocytes and the perfused rat liver is competitively inhibited by palmitate

American Journal of Physiology - Gastrointestinal and Liver Physiology

Volumn 270, Issue 2 33-2, 1996, Pages

  Sorrentino, Dario ^a   Stump, Decherd D ^b   Van Ness, Karen ^b   Simard, André ^b   Schwab, Andreas J ^b   Zhou, Sheng Li ^b   Goresky, Carl A ^b   Berk, Paul D ^b  

^a UNIVERSITY HOSPITAL OF UDINE   (Italy)

^b NONE

Author keywords

facilitated transport; free fatty acids; transhepatic indicator dilution technique

Indexed keywords

BOVINE SERUM ALBUMIN; LONG CHAIN FATTY ACID; OLEIC ACID; PALMITIC ACID; TRITIUM;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; COMPETITIVE INHIBITION; CONTROLLED STUDY; FATTY ACID TRANSPORT; INDICATOR DILUTION CURVE; LIVER CELL; LIVER PERFUSION; MALE; MICHAELIS CONSTANT; NONHUMAN; PRIORITY JOURNAL; RAT; STEADY STATE;

EID: 0029964478     PISSN: 01931857     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajpgi.1996.270.2.g385     Document Type: Article

References (38)

1. Abumrad, N. A., J. H. Park, and C. R. Park. Permeation of long chain fatty acid into adipocytes. Kinetics, specificity, and evidence for involvement of a membrane protein. J. Biol. Chem. 259: 8945-8953, 1984.
2. Berk, P. D., H. Wada, H. Horio, B. J. Potter, D. Sorrentino, S.-L. Zhou, L. M. Isola, D. Stump, C.-L. Kiang, and S. Thung. Plasma membrane fatty acid binding protein and mitochondrial glutamic-oxaloacetic transaminase are related. Proc. Natl. Acad. Sci. USA 87: 3484-3488, 1990.
3. Black, P. N., B. Said, C. R. Ghosn, J. R. Beach, and W. D. Nunn. Purification and characterization of an outer membrane-bound protein involved in long-chain fatty acid transport in Escherichia coli. J. Biol. Chem. 262: 1412-1419, 1987.
4. Cooper, R., N. Noy, and D. Zakim. A physical-chemical model for cellular uptake of fatty acids: prediction of intracellular pool sizes. Biochemistry 26: 5890-5896, 1987.
5. Cooper, R. B., N. Noy, and D. Zakim. Mechanism for binding of fatty acids to hepatocyte plasma membranes. J Lipid Res, 30: 1719-1726, 1989.
6. Ferraresi-Filho, O., M. L. Ferraresi, J. Constantin, E. L. Ishii-Iwamoto, A. J. Schwab, and A. Bracht. Transport and metabolism of palmitate in the rat liver. Net flux and unidirectional fluxes across the cell membrane. Biochim. Biophys. Acta 1103: 239-249, 1992.
7. Gores, G. J., L. J. Kost, and N. F. LaRusso. The isolated perfused rat liver: conceptual and practical considerations. Hepatology 6: 511-516, 1986.
8. Goresky, C. A. A method for determining linear sinusoidal and extravascular volumes. Am. J. Physiol. 204: 626-640, 1963.
9. Goresky C. A., G. G. Bach, D. Cousineau, A. J. Schwab, C., Rose, S. Lee, and S. Goresky. Handling of tracer norepinephrine by the dog liver. Am. J. Physiol. 256 (Gastrointest. Liver Physiol. 19): G107-G123, 1989.
10. Goresky, C. A., G. G. Bach, and B. E. Nadeau. On the uptake of materials by the intact liver: the concentrative transport of rubidium-86. J. Clin. Invest. 52: 975-990, 1973.
11. Goresky, C. A., G. G. Bach, and A. J. Schwab. Distributed-in-space product formation in vivo: linear kinetics. Am. J. Physiol. 264 (Heart Circ. Physiol. 33): H2029-H2050, 1993.
12. Hems, R., B. D. Ross, M. N. Berry, and H. A. Krebs. Gluconeogenesis in the perfused rat liver. Biochem. J. 101: 284-292, 1966.
13. Isola, L. M., S.-L. Zhou, C.-L Kiang, D. D. Stump, M. W. Bradbury, and P. D. Berk. 3T3 fibroblasts transfected with a cDNA for mitochondrial aspartate aminotransferase cDNA express plasma membrane fatty acid binding protein and saturable fatty acid uptake. Proc. Natl. Acad. Sci. USA 92: 9866-9870, 1995.
14. Landaw, E. M., and J. J. Distefano III. Multiexponential, multicompartmental, and noncompartmental modeling. II. Data analysis and statistical considerations. Am. J. Physiol. 246 (Regulatory Integrative Comp. Physiol. 15): R665-R667, 1984.
15. Noy, N., T. M. Donnelly, and D. Zakim. Physical-chemical model for the entry of water-insoluble compounds into cells. Studies of fatty acid uptake by the liver. Biochemistry 25: 2013-2021, 1986.
16. Potter, B. J., and P. D. Berk. Liver plasma membrane fatty acid binding protein. In: Hepatic Organic Anion Transport and Bile Secretion, edited by N. Tavoloni and P. D. Berk. New York: Raven, 1993. p. 253-267.
17. Richieri, G. V., and A. M. Kleinfeld. Unbound free fatty acid levels in human serum. J. Lipid Res. 36: 229-240, 1995.
18. Rose, H., M. Conventz, Y. Fischer, E. Jüngling, T. Hennecke, and H. Kammermeier. Long chain fatty acid binding to albumin: reevaluation with directly measured concentrations. Biochim. Biophys. Acta 1215: 321-326, 1994.
19. Sorrentino, D., and P. D. Berk. Free fatty acids, albumin and the sinusoidal plasma membrane: concepts, trends and controversies. In: Hepatic Organic Anion Transport and Bile Secretion, edited by N. Tavoloni and P. D. Berk. New York: Raven, 1993, p. 197-210.
20. Sorrentino, D., R. B. Robinson, C.-L. Kiang, and P. D. Berk. At physiologic albumin/oleate concentrations oleate uptake by isolated hepatocytes, cardiac myocytes and adipocytes is a saturable function of the unbound oleate concentration. Uptake kinetics are consistent with the conventional theory. J. Clin. Invest 84: 1325-1333, 1989.
21. Sorrentino, D., K Van Ness, D. Stump, and P. D. Berk. Oleate uptake kinetics in the perfused rat liver are consistent with pseudofacilitation by albumin. J. Hepatol. 21: 551-559, 1994.
22. Sorrentino, D., S.-L. Zhou, E. Kokkotou, and P. D. Berk. Sex differences in hepatic fatty acid uptake reflect a greater affinity of the transport system in females. Am. J. Physiol. 263 (Gastrointest. Liver Physiol. 26): G380-G385, 1992.
23. Spector, A. A. Structure and lipid binding properties of serum albumin. Methods Enzymol. 128: 320-339, 1986.
24. Spector, A. A., J. A. Fletcher, and J. D. Ashbrook. Analysis of long chain free fatty acid binding to bovine serum albumin by determination of stepwise equilibrium constants. Biochemistry 10: 3229-3232, 1971.
25. Storch, J. Mechanism for binding of fatty acids to hepatocyte plasma membranes: a different interpretation. Hepatology 12: 1447-1449, 1990.
26. Stremmel, W. Uptake of fatty acids by jejunal mucosal cells is mediated by a fatty acid binding membrane protein. J. Clin. Invest. 82: 2001-2010, 1988.
27. Stremmel, W. Fatty acid uptake by isolated rat heart myocytes represents a carrier-mediated transport process. J. Clin. Invest. 81: 844-852, 1988.
28. 14C]oleate reflects membrane transport rather than intracellular metabolism or binding. Proc. Natl. Acad. Sci. USA 83: 3086-3090, 1986.
29. Stremmel, W., G. Strohmeyer, and P. D. Berk. Hepatocellular uptake of oleate is energy dependent, sodium linked, and inhibited by an antibody to a hepatocyte plasma membrane fatty acid binding protein. Proc. Natl. Acad. Sci. USA 83: 3584-3588, 1986.
30. Stump, D., R. M. Nunes, D. Sorrentino, and P. D. Berk. Characteristics of oleate binding to liver plasma membranes and its uptake by isolated hepatocytes. J. Hepatol. 16: 304-315, 1992.
31. Stump, D. D., S.-L. Zhou, and P. D. Berk. Comparison of plasma membrane FABP and mitochondrial isoform of aspartate aminotransferase from rat liver. Am. J. Physiol. 265 (Gastrointest. Liver Physiol. 28): G894-G902, 1993.
32. Trimble, M. E. Mediated transport of long chain fatty acids by rat renal basolateral membranes. Am. J. Physiol. 257 (Renal Fluid Electrolyte Physiol 26): F539-F546, 1989.
33. Turcotte, L. P., B. Kiens, and E. A. Richter. Saturation kinetics of palmitate uptake in perfused skeletal muscle. FEBS Lett. 279: 327-329, 1991.
34. Van Ness, K., N. D. Theise, M. E. Schwartz, and C. A. Miller. Parasympathetic tone alterations during rat hepatectomy: preservation/harvest injury. Transpl. Proc. 25: 1938-1939, 1993.
35. Wilkinson, G. R. Clearance approaches in pharmacology. Pharmacol. Rev. 39: 1-47, 1987.
36. Wosilait, W. D., and P. Nagy. A method of computing drug distribution in plasma using stepwise association constants. Clofibrate as an illustrative example. Comput. Programs Biomed. 6: 142-148, 1976
37. Zhou, S.-L., D. Stump, L. M. Isola, and P. D. Berk. Constitutive expression of a saturable transport system for non-esterified fatty acids in Xenopus laevis oocytes. Biochem. J. 297: 315-319, 1994.
38. Zhou, S.-L., D. Stump, D. Sorrentino, B. J. Potter, and P. D. Berk. Adipocyte differentiation of 3T3-L1 cells involves augmented expression of a 43-kDa plasma membrane fatty acid binding protein. J. Biol. Chem. 267: 14456-14461, 1992.