Biophysical characterization of recombinant proteins expressing the leucine zipper-like domain of the human immunodeficiency virus type l transmembrane protein gp41

Journal of Virology

Volumn 70, Issue 5, 1996, Pages 2982-2991

  Shugars, Diane C ^a   Wild, Carl T ^a   Greenwell, Teresa K ^a   Matthews, Thomas J ^a  

^a DUKE UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GLYCOPROTEIN GP 41;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; CONFORMATIONAL TRANSITION; HUMAN IMMUNODEFICIENCY VIRUS 1; NONHUMAN; OLIGOMERIZATION; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN CROSS LINKING; PROTEIN SYNTHESIS REGULATION; VIRUS DNA CELL DNA INTERACTION; VIRUS EXPRESSION;

AMINO ACID SEQUENCE; CELL FUSION; CELL LINE; CHROMATOGRAPHY, GEL; CLONING, MOLECULAR; CROSS-LINKING REAGENTS; GENES, ENV; HIV ENVELOPE PROTEIN GP41; HIV-1; HUMANS; LEUCINE ZIPPERS; LIGHT; MACROMOLECULAR SUBSTANCES; MOLECULAR SEQUENCE DATA; PEPTIDE FRAGMENTS; PROTEIN CONFORMATION; RECOMBINANT FUSION PROTEINS; RECOMBINANT PROTEINS; SCATTERING, RADIATION; SUCCINIMIDES;

EID: 0029964418     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/jvi.70.5.2982-2991.1996     Document Type: Article

References (55)

1. Bernstein, H. B., S. P. Tucker, S. R. Kar, S. A. McPherson, D. T. McPherson, J. W. Dubay, J. Lebowitz, R. W. Company and E. Hunter. 1995. Oligomerization of the hydrophobic heptad repeat of gp41. J. Virol. 69:2745-2750.
2. Blondel, A., and H. Bedouelle. 1990. Export and purification of a cytoplasmic dimeric protein by fusion to the maltose-binding protein of Escherichia coli Eur. J Biochem. 193:325-330.
3. Britton, P. 1991 Coronavirus motif. Nature (London) 353:394.
4. Buckland, R., E. Malvoisin, P. Beauverger, and F. Wild. 1992 A leucine zipper structure present in the measles virus fusion protein is not required for its tetramerization but is essential for fusion. J Gen. Virol 73:1703-1707.
5. Buckland, R., and F. Wild. 1989. Leucine zipper extends. Nature (London) 338:547.
6. Bullough, P. A., F. M. Hughson, J. J. Skehel, and D. C. Wiley. 1994. Structure of influenza haemagglutinin at the pH of membrane fusion Nature (London) 371:37-43.
7. Carr, C. M., and P. S. Kim. 1993. A spring-loaded mechanism for the conformational change of influenza hemagglutinin. Cell 73:823-832.
8. Chambers, P., C. R. Pringle, and A. J. Easton. 1990. Heptad repeat sequences are located adjacent to hydrophobic regions in several types of virus fusion glycoproteins. J Gen. Virol. 71:3075-3080.
9. Chen, C.-H., T. J. Matthews, C. B. McDanal, D. P. Bolognesi, and M. L. Greenberg. 1995. A molecular clasp in the human immunodeficiency virus (HIV) type 1 TM protein determines the ann-HIV activity of gp41 derivatives: implication for viral fusion. J Virol 69:3771-3777.
10. Chen, S. S.-L. 1994. Functional role of the zipper motif region of human immunodeficiency virus type 1 transmembrane protein gp41. J. Virol. 68:2002-2010.
11. Chen, S. S.-L, C.-N. Lee, W.-R. Lee, K. McIntosh, and T.-H. Lee. 1993. Mutational analysis of the leucine zipper-like motif of the human immunodeficiency virus type 1 envelope transmembrane glycoprotein. J Virol. 67:3615-3619.
12. Cohen, C., and D. A. D. Parry. 1986. ̧-helical coiled coils-a widespread motif in proteins. Trends Biol. Sci. 11:245-248.
13. Crick, F. H. C. 1953. The packing of α-helices: simple coiled coils Acta Crystallogr. 6:689-697.
14. Crowl, R., K. Ganguly, M. Gordon, R. Conroy, M. Schaber, R. Kramer, G. Shaw, F. Wong-Staal, and E. P. Reddy. 1985. HTLV-III env gene products synthesized in E coli are recognized by antibodies present in the sera of AIDS patients. Cell 41:979-986.
15. Delwart, E. L., and G. Mosialos. 1990. Retroviral envelope glycoproteins contain a "leucine zipper"-like repeat. AIDS Res. Hum. Retroviruses 6:703-706.
16. Dewar, R. L., M. B. Vasudevachari, V. Natarjan, and N. P. Salzman. 1989. Biosynthesis and processing of human immunodeficiency virus type 1 envelope glycoproteins: effects of monensin on glycosylation and transport J. Virol. 63:2452-2456.
17. Doms, R. W., R. A. Lamb, J. K. Rose, and A. Helenius. 1993. Folding and assembly of viral membrane proteins. Virology 193:545-562.
18. Dubay, J. W., S. J. Roberts, B. Brody, and E. Hunter. 1992. Mutations in the leucine zipper of the human immunodeficiency virus type 1 transmembrane glycoprotein affect fusion and infectivity. J. Virol. 66:4748-1756.
19. Earl, P. L., R. W. Doms, and B. Moss. 1990. Oligomeric structure of the human immunodeficiency virus type 1 envelope glycoprotein. Proc Natl. Acad Sci. USA 87:648-652.
20. Earl, P. L., and B. Moss. 1993 Mutational analysis of the assembly domain of the HIV-1 envelope glycoprotein. AIDS Res Hum. Retroviruses 9:589-594.
21. Earl, P. L., B. Moss, and R. W. Doms. 1991 Folding, interaction with GRP78-BiP, assembly, and transport of the human immunodeficiency virus type 1 envelope protein J. Virol. 65:2047-2055.
22. Edelhoch, H. 1967 Spectroscopic determination of tryptophan and tyrosine in proteins. Biochemistry 6:1948-1954.
23. Ellenberger, T. E., C. J. Brandi, K. Struhl, and S. C. Harrison. 1992. The GCN4 basic region leucine zipper binds DNA as a dimer of uninterrupted α-helices: crystal structure of the protein-DNA complex. Cell 71:1223-1237.
24. Gallaher, W. R., J. M. Ball, R. F. Garry, M. C. Griffin, and R. C. Momelaro. 1989. A general model for the transmembrane proteins of HIV and other retroviruses. AIDS Res Hum. Retroviruses 5:431-440.
25. Gelderblom, H. R., E. H. S. Hausmann, M. Ozel, G. Pauli, and M. A. Koch. 1987. Fine structure of human immunodeficiency virus (HIV) and immunolocalization of structural proteins. Virology 156:171-176.
26. Guan, C., P. Li, P. D. Riggs, and H. Inouye. 1987. Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein. Gene 67:21-30.
27. Ho, S. N., H. D. Hunt, R. M. Horton, J. K. Pullen, and L. R. Pease. 1989 Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 77:51-59.
28. Landschulz, W. H., P. F. Johnson, and S. L. MeKnight. 1988. The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins. Science 240:1759-1764.
29. Laue, T. M., B. D. Shah, T. M. Ridgeway, and S. L. Pelletier. 1992 Computer-aided interpretation of analytical sedimentation data for proteins, p. 90-125. In S. E Harding, A J. Rowe, and J. C. Horton (ed.). Analytical ultracentrifugation in biochemistry and polymer science. The Royal Society of Chemistry, Cambridge.
30. Malim, M. H., J. Hauber, R. Fenrick, and B. R. Cullen. 1988 Immunodeficiency virus rev tranas-activator modulates the expression of the viral regulatory genes Nature (London) 335:181-183.
31. Myers, G., B. Korber, S. Wain-Hobson, R. F. Smith, and G. N. Pavlakis. 1993 Human retroviruses and AIDS 1993 I-II: a compilation and analysis of nucleic acid and amino acid sequences Los Alamos National Laboratory. Los Alamos, N. Mex.
32. Nagia, K., and H. C. Thogersen. 1987. Synthesis and sequence-specific proteolysis of hybrid proteins produced in Escherichia coli. Methods Enzymol. 153:461-481
33. O'Shea, E. K., J. D. Klemm, P. S. Kim, and T. Alber. 1991. X-ray structure of the GCN4 leucine zipper, a two-stranded, parallel coiled coil Science 254:539-544.
34. O'Shea, E. K., R. Rutkowski, and P. S. Kim. 1989. Evidence that the leucine zipper is a coiled coil. Science 243:538-542
35. Owens, R. J., and R. W. Compans. 1990. The human immunodeficiency, virus type 1 envelope glycoprotein precursor acquires aberrant intermolecular disulfide bonds that may prevent normal proteolytic processing. Virology 179:827-833
36. Peteranderl, R., and H. C. M. Nelson. 1992. Trimerization of the heal shock transcription factor by a triple-stranded α-helical coiled-coil. Biochemistry 31:12272-12276.
37. Pinter, A., W. J. Honnen, S. A. Tilley, C. Bona, H. Zaghouani, M. K. Gorny, and S. Zolla-Pazner. 1989 Oligomeric structure of gp41, the transmembrane protein of human immunodeficiency virus type 1. J. Virol. 63:2674-2679.
38. Popovic, M., M. G. Sarngadharan, E. Read, and R. C. Gallo. 1984 Detection, isolation and continuous production of cytopathic retroviruses (HILV-III) from patients with AIDS and pre-AIDS. Science 224:497-500
39. Poumbourios, P., W. El Ahmar, D. A. McPhee, and B. E. Kemp. 1995. Determinants of human immunodeficiency virus type 1 envelope glycopro-tein oligomeric structure. J. Virol. 69:1209-1218.
40. Poumbaurios, P., D. A. McPhee, and B. E. Kemp. 1992. Antibody epitopes sensitive to the state of human immunodeficiency virus type 1 gp41 oligomer-ization map to a putative α-helical region. AIDS Res. Hum. Retroviruses 8:2055-2062.
41. Rabenstein, M., and Y.-Y. Shin. 1995. A peptide from the heptad repeat of human immunodeficiency virus gp41 shows both membrane binding and coiled-coil formation. Biochemistry 34:13390-13397.
42. Rodriguez, P. L., and L. Carrasco. 1995. Improved factor Xa cleavage of tusion proteins containing maltose binding protein. BioTechniques 18:238-243
43. Ross, P. D., F. B. Howard, and M. S. Lewis. 1991. Thermodynamics of antiparallel hairpin-double helix equilibria in DNA oligonucleotides from equilibrium ultracentrifugation. Biochemistry 30:6269-6275.
44. Sehachman, H. K. 1959. Ultracentrifugation in biochemistry. Academic Press, New York.
45. Schawaller, M., G. E. Smith, J. J. Skehel, and D. C. Wiley. 1989. Studies with crosslinking reagents on the oligomeric structure of the env glycoprotein of HIV Virology 172:367-369.
46. Spruce, A. E., A. Iwata, and W. Almers. 1991. The first milliseconds of the pore formed by a fusogenic viral envelope protein during membrane fusion. Proc. Natl. Acad. Sci. USA 88:3623-3627.
47. Spurlino, J. C., G.-Y. Lu, and F. A. Quiocho. 1991. The 23-Å resolution structure of the maltose- or maltodextrin-binding protein, a primary receptor of bacterial active transport and chemotaxis. J Biol. Chem. 266:5202-5219.
48. Takagi, H., Y. Morinaga, M. Tsuchiya, G. Ikemura, and M. Inouye. 1988. Control of folding of proteins secreted by a high expression secretion vector, pIN-HI-ompA: 16-fold increase in production of active subtilisin E in Escherichia coli. Bio/Technology 6:948-950.
49. Thomas, D. J., J. S. Wall, J. F. Hainfeld, M. Kaczorek, F. P. Booy, B. L. Trus, F. A. Eiserling, and A. C. Steven. 1991 gp160, the envelope glycoprotein of human immunodeficiency virus type 1. is a dimer of 125-kilodalton subunits stabilized through interactions between their gp41 domains. J. Virol. 65:3797-3803.
50. White, J. M. 1992. Membrane fusion. Science 258:917-923.
51. Wild, C., J. W. Dubay, T. Greenwell, T. Baird, Jr., T. G. Oas, C. McDanal, E. Hunter, and T. Matthews. 1994 Propensity for a leucine zipper-like domain of human immunodeficiency virus type 1 gp41 to form oligomers correlates with a role in virus-induced fusion rather than assembly of the glycoprotein complex Proc. Natl. Acad. Sci. USA 91:12676-12680.
52. Wild, C., T. Oas, C. McDanal, D. Bolognesi, and T. Matthews. 1992. A synthetic peptide inhibitor of human immunodeficiency virus replication: correlation between solution structure and viral inhibition. Proc. Natl. Acad. Sci. USA 89:10537-10541.
53. Wiley, D. C., and J. J. Skehel. 1987. The structure and function of the hemagglutinin membrane glycoprotein of influenza virus. Annu. Rev. Biochem. 56:365-394
54. Wilson, I. A., J. J. Skehel, and D. C. Wiley. 1981. Structure of the hemagglutinin membrane glycoprotein of influenza virus at 3Å resolution. Nature (London) 289:366-373
55. Yu, Y. G., D. S. King, and Y.-K. Shin. 1994. Insertion of a coiled-coil peptide from influenza virus hemagglutinin into membranes. Science 266:274-276.