Immunological characterization of a recombinant 27-kilodalton antigenic protein from Paracoccidioides brasiliensis

Clinical and Diagnostic Laboratory Immunology

Volumn 3, Issue 2, 1996, Pages 239-241

  Ortiz, B L ^a   Garcia, A M ^a   Restrepo, A ^a   McEwen, J G ^a  

^a Corp para Invest Biológicas ^*  (Colombia)

Author keywords

[No Author keywords available]

Indexed keywords

FUNGUS ANTIGEN; RECOMBINANT PROTEIN;

ARTICLE; CONTROLLED STUDY; CROSS REACTION; HUMAN; IMMUNOBLOTTING; MAJOR CLINICAL STUDY; PARACOCCIDIOIDES BRASILIENSIS; PRIORITY JOURNAL; SOUTH AMERICAN BLASTOMYCOSIS;

EID: 0029963607     PISSN: 1071412X     EISSN: None     Source Type: Journal    
DOI: 10.1128/cdli.3.2.239-241.1996     Document Type: Article

References (19)

1. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl (ed.). 1987. Current protocols in molecular biology, vol. 10, p. 10.01-10.85. Greene Publications Associates and Wiley & Sons, New York.
2. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-252.
3. Brummer, E., E. Castaneda, and A. Restrepo. 1993. Paracoccidioidomycosis: an update. Clin. Microbiol. Rev. 6:89-117.
4. Camargo, Z. P., C. Unterkircher, and L. R. Travassos. 1989. Identification of antigenic polypeptides of Paracocctidioides brasiliensis by immunoblotting. J. Med. Vet. Mycol. 27:407-412.
5. Cano, L. E., and A. Restrepo. 1987. Predictive value of serologic tests in the diagnosis and follow-up of patients with paracoccidioidomycosis. Rev. Inst. Med. Trop. Sao Paulo 29:276-283.
6. Casotto, M. 1990. Characterization of the cellular antigens of Paracoccidioides brasiliensis yeast form. J. Clin. Microbiol. 28:1188-1193.
7. Ferreira-da-Cruz, M. F., B. Galvāo-Castro, and C. Daniel-Ribeiro. 1992. Isolation and antigenicity of a 45-kilodalton Paracoccidioides brasiliensis immunodominant antigen. Infect. Immun. 60:2667-2671.
8. Galen, R. S., and S. R. Gambino. 1975. Beyond normality: the predictive value and efficiency of medical diagnoses. John Wiley & Sons, New York.
9. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680-685.
10. Mendes-Giannini, M. J. S., G. Del Negro, and A. M. Siqueira. 1994. Serodiagnosis, p. 345-357. In M. Franco, C. S. Lacaz, A. Restrepo-Moreno, and G. Del Negro (ed.), Paracoccidioidomycosis. CRC Press, Inc., Boca Raton, Ra.
11. Promega Corporation. 1991. Nucleic acid detection, purification and labeling, p. 125-130. In Promega protocols and applications guide. Promega Corporation, Madison, Wis.
12. Puccia, R., S. Schenkman, P. A. Gorin, and L. R. Travassos. 1986. Exocellular components of Paracoccidioides brasiliensis: identification of a specific antigen. Infect. Immun. 53:199-206.
13. Puccia, R., and L. R. Travassos. 1991. 43-kilodalton glycoprotein from Paracoccidioides brasiliensis: immunochemical reactions with sera from patients with paracoccidioidomycosis, histoplasmosis, or Jorge Lobo's disease. J. Clin. Microbiol. 29:1610-1615.
14. Restrepo, A. 1995. Paracoccidioides brasiliensis, p. 2386-2389. In G. L. Mandell, J. E. Bennett, and R. Dolin (ed.), Principles and practice of infectious diseases, 4th ed. Churchill Livingstone, London.
15. Restrepo, A., L. E. Cano, and M. T. Ochoa. 1984. A yeast-derived antigen from Paracoccidioides brasiliensis useful for serologic testing. Sabouraudia 22:23-29.
16. Restrepo, A., and B. Jimenez. 1980. Growth of Paracoccidioides brasiliensis yeast phase in a chemically defined medium. J. Clin. Microbiol. 12:279-281.
17. Short, J. M., and J. A. Sorge. 1992. In vivo excision properties of bacterio-phage λ Zap expression vector. Methods Enzymol. 216:495-508.
18. Taba, M. R. M., J. F. Da Silveira, L. R. Travassos, and S. Schenkman. 1989. Expression in Escherichia coli of a gene coding for epitopes of a diagnostic antigen of Paracoccidioides brasiliensis. Exp. Mycol. 13:223-230.
19. Taborda, C. P., and Z. P. Camargo. 1994. Diagnosis of paracoccidioidomycosis by dot immunobinding assay for antibody detection using the purified and specific antigen gp43. J. Clin. Microbiol. 32:554-556.