Expression and characterization of human perlecan domains I and II synthesized by baculovirus-infected insect cells

European Journal of Biochemistry

Volumn 241, Issue 3, 1996, Pages 827-834

  Groffen, Alexander J A ^a   Buskens, Carin A F ^a   Tryggvason, Karl ^b   Veerkamp, Jacques H ^c   Monnens, Leo A H ^a   Van Den Heuvel, Lambert P W J ^a  

^a RADBOUD UNIVERSITY NIJMEGEN   (Netherlands)

^b KAROLINSKA INSTITUTE   (Sweden)

^c RADBOUD UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

Baculovirus expression; Basic fibroblast growth factor; Chondroitin sulfate; Perlecan; Structure

Indexed keywords

COMPLEMENTARY DNA; PERLECAN; PROTEOGLYCAN;

AGAR GEL ELECTROPHORESIS; ANIMAL CELL; ARTICLE; BACULOVIRUS; CONTROLLED STUDY; DNA SEQUENCE; DRUG PURIFICATION; EXPRESSION VECTOR; GENE EXPRESSION; HYBRIDIZATION; IMMUNOBLOTTING; IN VITRO STUDY; INSECT; ION EXCHANGE CHROMATOGRAPHY; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN DOMAIN; SOUTHERN BLOTTING; TECHNIQUE;

ANIMALIA; ESCHERICHIA COLI; INSECTA; UNIDENTIFIED BACULOVIRUS;

EID: 0029963516     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1996.00827.x     Document Type: Article

References (45)

1. Kallunki, P. & Tryggvason, K. (1992) Human basement membrane heparan sulfate proteoglycan core protein: a 467-kDa protein containing multiple domains resembling elements of the low density lipoprotein receptor, laminin, neural cell adhesion molecules, and epidermal growth factor, J. Cell Biol. 116, 559-571.
2. Murdoch, A. D., Dodge, G. R., Cohen, I., Tuan, R. S. & Iozzo, R. V. (1992) Primary structure of the human heparan sulfate proteoglycan from basement membrane (HSPG2/perlecan). A chimeric molecule with multiple domains homologous to the low density lipoprotein receptor, laminin, neural cell adhesion molecules, and epidermal growth factor, J. Biol. Chem. 267, 8544-8557.
3. Murdoch, A. D., Liu, B., Schwarting, R., Tuan, R. S. & Iozzo, R. V. (1994) Widespread expression of perlecan proteoglycan in basement membranes and extracellular matrices of human tissues as detected by a novel monoclonal antibody against domain III and by in situ hybridization, J. Histochem. Cytochem. 42, 239-249.
4. Dodge, G. R., Kovalszky, I., Chu, M. L., Hassell, J. R., McBride, O. W., Yi, H. F. & Iozzo, R. V. (1991) Heparan sulfate proteoglycan of human colon: partial molecular cloning, cellular expression, and mapping of the gene (HSPG2) to the short arm of human chromosome 1, Genomics 10, 673-680.
5. Cohen, I. R., Grassel, S., Murdoch, A. D. & Iozzo, R. V. (1993) Structural characterization of the complete human perlecan gene and its promoter, Proc. Natl Acad. Sci. USA 90, 10404-10408.
6. Battaglia, C., Mayer, U., Aumailley, M. & Timpl, R. (1992) Basement-membrane heparan sulfate proteoglycan binds to laminin by its heparan sulfate chains and to nidogen by sites in the protein core, Eur. J. Biochem. 208, 359-366.
7. Heremans, A., de Cock, B., Cassiman, J. J., Van den Berghe, H. & David, G. (1990) The core protein of the matrix-associated heparan sulfate proteoglycan binds to fibronectin, J. Biol. Chem. 265, 8716-8724.
8. Laurie, G. W., Bing, J. T. Kleinman, H. K., Hassell, J. R., Aumailley, M., Martin, G. R. & Feldmann, R. J. (1986) Localization of binding sites for laminin, heparan sulfate proteoglycan and fibronectin on basement membrane (type IV) collagen, J. Mol. Biol. 189, 205-216.
9. Chakravarti, S., Horchar, T. Jefferson, B., Laurie, G. W. & Hassell, J. R. (1995) Recombinant domain III of perlecan promotes cell attachment through its RGDS sequence, J. Biol. Chem. 270, 404-409.
10. Hayashi, K., Madri, J. A. & Yurchenco, P. D. (1992) Endothelial cells interact with the core protein of basement membrane perlecan through beta 1 and beta 3 integrins: an adhesion modulated by glycosaminoglycan, J. Cell Biol. 119, 945-959.
11. Kokenyesi, R. & Silbert, J. E. (1995) Formation of heparan sulfate or chondroitin/dermatan sulfate on recombinant domain I of mouse perlecan expressed in Chinese hamster ovary cells, Biochem. Biophys. Res. Commun. 211, 262-267.
12. Kanwar, Y. S., Linker, A. & Farquhar, M. G. (1980) Increased permeability of the glomerular basement membrane to ferritin after removal of glycosaminoglycans (heparan sulfate) by enzyme digestion, J. Cell. Biol. 86, 688-693.
13. Aviezer, D., Hecht, D., Safran, M., Eisinger, M., David, G. & Yayon, A. (1994) Perlecan, basal lamina proteoglycan, promotes basic fibroblast growth factor-receptor binding, mitogenesis, and angiogenesis, Cell 79, 1005-1013.
14. Gallagher, J. T. (1994) Heparan sulphates as membrane receptors for the fibroblast growth factors, Eur. J. Clin. Chem. Clin. Biochem. 32, 239-247.
15. Schlessinger, J., Lax, I. & Lemmon, M. (1995) Regulation of growth factor activation by proteoglycans: what is the role of the low affinity receptors? Cell 83, 357-360.
16. Guillonneau, X., Tassin, J., Berrou, F., Bryckaert, M., Courtois, Y. & Mascarelli, F. (1996) In vitro changes in plasma membrane heparan sulfate proteoglycans and in perlecan expression participate in the regulation of fibroblast growth factor 2 mitogenic activity, J. Cell. Physiol. 166, 170-187.
17. Turnbull, J. E., Fernig, D. G., Ke, Y., Wilkinson, M. C. & Gallagher, J. T. (1992) Identification of the basic fibroblast growth factor binding sequence in fibroblast heparan sulfate, J. Biol. Chem. 267, 10337-10341.
18. Pejler, G., Backstrom, G., Lindahl, U., Paulsson, M. Dziadek, M., Fujiwara, S. & Timpl, R. (1987) Structure and affinity for antithrombin of heparan sulfate chains derived from basement membrane proteoglycans, J. Biol. Chem. 262, 5036-5043.
19. Mertens, G., Cassiman, J. J., Van den Berghe, H., Vermylen, J. & David, G. (1992) Cell surface heparan sulfate proteoglycans from human vascular endothelial cells. Core protein characterization and antithrombin III binding properties, J. Biol. Chem. 267, 20435-20443.
20. Eisenberg, S., Sehayek, E., Olivecrona, T. & Vlodavsky, I. (1992) Lipoprotein lipase enhances binding of lipoproteins to heparan sulfate on cell surfaces and extracellular matrix. J. Clin. Invest. 90, 2013-2021.
21. Olivecrona, G. & Olivecrona, T. (1995) Triglyceride lipases and atherosclerosis, Curr. Opin. Lipidol. 6, 291-305.
22. Iozzo, R. V. (1994) Perlecan: a gem of a proteoglycan, Matrix. Biol. 14, 203-208.
23. O'Reilly, D. R., Miller, L. K. & Luckow, V. A. (1992) Baculovirus expression vectors: a laboratory manual, Freeman and Company, New York.
24. Miller, L. K. (1993) Baculoviruses: high-level expression in insect cells, Curr. Opin. Genet. Dev. 3, 97-101.
25. Thomsen, D. R., Post, L. E. & Elhammer, A. P. (1990) Structure of O-glycosidically linked oligosaccharides synthesized by the insect cell line Sf9, J. Cell Biochem. 43, 67-79.
26. Graner, M., Stupka, K. & Karr, T. L. (1994) Biochemical and cytological characterization of DROP-1: a widely distributed proteoglycan in Drosophila, Insect Biochem. Mol. Biol. 24, 557-567.
27. Inoue, H., Nojima, H. & Okayama, H. (1990) High efficiency transformation of Escherichia coli with plasmids, Gene 96, 23-28.
28. Smith, G. E. & Summers, M. D. (1978) Analysis of baculovirus genomes with restriction endonucleases, Virology 89, 517-527.
29. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989) Molecular cloning, a laboratory manual, 2nd edn, Cold Spring Harbor Laboratory Press, Cold Spring Harbor NY.
30. Reubsaet, F. A., Langeveld, J. P. & Veerkamp, J. H. (1985) Glycosaminoglycan content of glomerular and tubular basement membranes of various mammalian species, Biochim. Biophys. Acta 838, 144-150.
31. Harlow, E. & Lane, D. (1988) Antibodies, a laboratory manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor NY.
32. Smith, D. B. & Johnson, K. S. (1988) Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione-S-transferase, Gene 67, 31-40.
33. Van den Heuvel, L. P., Van den Born, J., Veerkamp, J. H., Van de Velden, T. J., Schenkels, L., Monnens, L. A., Schröder, C. H. & Berden, J. H. (1990) Heparan sulfate proteoglycan from human tubular basement membrane. Comparison with this component from the glomerular basement membrane, Biochem. Biophys. Acta 1025, 67-76.
34. Van de Lest, C. H., Versteeg, E. M., Veerkamp, J. H. & Van Kuppevelt, T. H. (1994) Quantification and characterization of glycosaminoglycans at the nanogram level by a combined azure A-silver staining in agarose gels, Anal. Biochem. 221, 356-361.
35. Huh, N. E. & Weaver, R. F. (1990) Identifying the RNA polymerases that synthesize specific transcripts of the Autographa californica nuclear polyhedrosis virus, J. Gen. Virol. 71, 195-201.
36. Westwood, J. A., Jones, I. M. & Bishop, D. H. (1993) Analyses of alternative poly(A) signals for use in baculovirus expression vectors, Virology 195, 90-99.
37. Ooi, B. G. & Miller, L. K. (1990) Transcription of the baculovirus polyhedrin gene reduces the levels of an antisense transcript initiated downstream, J. Virol. 64, 3126-3129.
38. Pham, D. Q. & Sivasubramanian, N. (1992) In vivo transcriptional analysys of three baculovirus genes: evidence of homology between viral and host transcripts, Virology 190, 288-297.
39. Van den Heuvel, L. P., Van den Born, J., Van de Velden, T. J., Veerkamp, J. H., Monnens, L. A., Schroder, C. H. & Berden, J. H. (1989) Isolation and partial characterization of heparan sulfate proteoglycan from the human glomerular basement membrane, Biochem. J. 264, 457-465.
40. Van de Lest, C. H., Versteeg, E. M., Veerkamp, J. H. & Van Kuppevelt, T. H. (1995) Digestion of proteoglycans in porcine pancreatic elastase-induced emphysema in rats, Eur. Respir. J. 8, 238-245.
41. Aviezer, D., Levy, E., Safran, M., Svahn, C., Buddecke, E., Schmidt, A., David, G., Vlodavsky, I. & Yayon, A. (1994) Differential structural requirements of heparin and heparan sulfate proteoglycans that promote binding of basic fibroblast growth factor to its receptor, J. Biol. Chem. 269, 114-121.
42. Zhang, L., David, G. & Esko, J. D. (1995) Repetitive Ser-Gly sequences enhance heparan sulfate assembly in proteoglycans, J. Biol. Chem. 270, 27127-27135.
43. Danielson, K. G., Martinez Hernandez, A., Hassell, J. R. & Iozzo, R. V. (1992) Establishment of a cell line from the EHS tumor: biosynthesis of basement membrane constituents and characterization of a hybrid proteoglycan containing heparan and chondroitin sulfate chains, Matrix 12, 22-35.
44. Van Det, N. F., Van den Born, J., Tamsma, J. T., Verhagen, N. A., Van den Heuvel, L. P., Berden, J. H., Bruijn, J. A., Daha, M. R. & Van der Woude, F. J. (1995) Proteoglycan production by human glomerular visceral epithelial cells and mesangial cells in vitro, Biochem. J. 307, 759-768.
45. Paulsson, M., Yurchenco, P. D., Ruben, G. C., Engel, J. & Timpl, R. (1987) Structure of low density heparan sulfate proteoglycan isolated from a mouse tumor basement membrane, J. Mol. Biol. 197, 297-313.