Crystallization and preliminary X-ray diffraction studies of α-amylase from the antarctic psychrophile Alteromonas haloplanctis A23

Protein Science

Volumn 5, Issue 10, 1996, Pages 2128-2129

  Aghajari, Nushin ^a   Feller, Georges ^b   Gerday, Charles ^b   Haser, Richard ^a,c  

^a CNRS   (France)

^b UNIVERSITY OF LIÈGE   (Belgium)

^c CNRS and Aix Marseille University   (France)

Author keywords

extremophiles; psychrophilic enzymes; X ray crystallography; amylase

Indexed keywords

AMYLASE;

ADAPTATION; ARTICLE; CATALYSIS; CRYSTALLIZATION; ENZYME ACTIVE SITE; ENZYME PURIFICATION; ENZYME STRUCTURE; GRAM NEGATIVE BACTERIUM; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONFORMATION; SUPERNATANT; X RAY DIFFRACTION;

ALTEROMONAS; BACTERIA (MICROORGANISMS); NEGIBACTERIA; PSEUDOALTEROMONAS HALOPLANKTIS; SUS SCROFA;

EID: 0029962635     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1002/pro.5560051021     Document Type: Article

References (14)

1. Arakawa T, Bhat R, Timasheff SN. 1990. Why preferential hydration does not always stabilize the native structure of globular proteins. Biochemistry 29:1924-1931.
2. Arakawa T, Timasheff SN. 1985. Theory of protein solubility. Methods Enzymol 114:49-77.
3. Brayer GD, Luo Y, Withers SG. 1995. The structure of human pancreatic α-amylase at 1.8 Å resolution and comparisons with related enzymes. Protein Sci 4:1730-1742.
4. Brünger AT. 1993. X-PLOR version 3.1. New Haven, Connecticut: Yale University.
5. CCP4. 1994. Collaborative Computing Project Number 4. The CCP4 suite: Programs for protein crystallography. Acta Crystallogr D 50:760-763.
6. Feller G, Lonhienne T, Deroanne C, Libioulle C, Van Beeumen J, Gerday Ch. 1992. Purification, characterization and nucleotide sequence of the thermolabile α-amylase from the antarctic psychrotroph Alteromonas haloplanctis A23. J Biol Chem 267:5217-5221.
7. Feller G, Narinx E, Arpigny JL, Aittaleb M, Baise E, Genicot S, Gerday Ch. 1996. Enzymes from psychrophilic organisms. FEMS Microbiol Rev 18:189-202.
8. Feller G, Payan F, Theys F, Qian M, Haser R, Gerday Ch. 1994. Stability and structural analysis of α-amylase from the antarctic psychrophile Alteromonas haloplanctis A23. Eur J Biochem 222:441-447.
9. Hochachka PW, Somero GN. 1984. Biochemical adaptations. Princeton, New Jersey: Princeton University Press.
10. Jancarik J, Kim SH. 1991. Sparse matrix sampling: A screening method for crystallization of proteins. J Appl Crystallogr 24:409-411.
11. Matthews BW. 1968. Solvent content of protein crystals. J Mol Biol 33:491-497.
12. Minor W. 1993. XDISPLAYF program. West Lafayette, Indiana: Purdue University.
13. Otwinowski Z. 1993. Oscillation data reduction program. 1993. In: Proceedings of the CCP4 study weekend: Data collection and processing, 29-30 January 1993. Compiled by: L. Sawyer, N. Isaacs, and S. Bailey. England: SERC Daresbury Laboratory, pp 56-62.
14. Qian M, Haser R, Payan F. 1993. Structure and molecular model refinement of pig pancreatic α-amylase at 2.1 Å resolution. J Mol Biol 231:785-799.