Probing chromatin structure in the early phases of apoptosis

Cell Proliferation

Volumn 29, Issue 7, 1996, Pages 427-436

  Ferlini, C ^a   Biselli, R ^a   Scambia, G ^b   Fattorossi, A ^a,c  

^a DASRS   (Italy)

^b CATHOLIC UNIVERSITY   (Italy)

^c ISTITUTO SUPERIORE DI SANITÀ   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; ETHIDIUM BROMIDE; FLUORESCEIN ISOTHIOCYANATE; NUCLEAR PROTEIN; PROPIDIUM IODIDE;

APOPTOSIS; ARTICLE; CELL NUCLEUS; CHROMATIN STRUCTURE; CONTROLLED STUDY; DNA DETERMINATION; DNA PROBE; HUMAN; HUMAN CELL; LIGHT SCATTERING; MONONUCLEAR CELL; NORMAL HUMAN; SPECTROPHOTOMETRY;

EID: 0029962553     PISSN: 09607722     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2184.1996.tb00985.x     Document Type: Article

References (23)

1. BRUNO S, DEL BINO D, LASSOTA P, GIARETTI W, DARZYNKIEWICZ Z. (1992a) Inhibitors of proteases prevent endonucleolysis accompanying apoptotic cell death. Leukemia 11, 1113.
2. 0 cells and is prevented by inhibitors of proteases. Oncol. Res. 4, 29.
3. BURSCH W, OBERHAMMER F, SCHULTE-HEMANN R. (1992) Cell death by apoptosis and its protective role against disease. Trends Pharmacol. Sci. 13, 245.
4. COHEN JJ. (1991) Programmed cell death in immune system. Adv. Immunol. 50, 55.
5. DARZYNKIEWICZ Z, TRAGANOS F, KAPUCINSKI J, STAIANO-COICO L, MELAMED MR. (1984) Accessibility of DNA in situ to various fluorochromes: Relationship to chromatin changes during erythroid differentiation of Friend leukemia cells. Cytometry 5, 355.
6. DARZYNKIEWICZ Z, BRUNO S, DEL BINO G, GORCZYCA W, HOTZ MA, LASSOTA P, TRAGANOS F. (1992) Features of apoptotic cells measured by flow cytometry. Cytometry 13, 795.
7. DYSON JED, MCLAUGHLIN JB, SURREY CR, SIMMONS DM, DANIEL J. (1987) Effects of hyperthermia, irradiation and cytotoxic drugs on fluorescein isothiocyanate staining intensity for flow cytofluorometry. Cytometry 8, 26.
8. EARNSHAW WC. (1995) Apoptosis: lessons from in vitro systems. Trends in Cell Biol. 5, 217.
9. FERLINI C, DI CESARE S, RAINALDI G et al. (1996) Flow cytometric analysis of the early phases of apoptosis by cellular and nuclear techniques. Cytometry 24. 106.
10. FOTHERGILL JE. (1969) Fluorochromes and their conjugation with proteins. In: Nairn RC. ed. Fluorescent Protein Tracing. Baltimore: Williams and Wilkins, pp. 5-54.
11. GILL JE, JOTZ MM, YOUNG SG, MODEST EJ, SENGUPTA SK. (1975) 7-amino actinomycin D as a cytochemical probe. I. Spectral properties. J. Histochem. Cytochem. 23, 793.
12. GORCZYCA W, GONG J, DARZYNKIEWICZ Z. (1993) Detection of DNA strand breaks in individual apoptotic cells by the in situ terminal deoxynucleotidyl transferase and nick translation assays. Cancer Res. 53, 1945.
13. HIGASHIKUBO R, WRIGHT WD, ROTI ROTI JL. (1992) Flow cytometric methods for studying isolated nuclei: DNA accessibility, to DNase I and protein-DNA content. Meth. Cell Biology 33, 315.
14. HOTZ MA, GONG J, TRAGANOS F, DARZYNKIEWICZ Z. (1994) Flow cytometric detection of apoptosis: comparison of the assays of in situ DNA degradation and chromatin changes. Cytometty 15, 237.
15. LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ. (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265.
16. NISINI R, MATRICARDI PMM, FATTOROSSI A, BISELLI R, D'AMELIO R. (1992) Presentation of superantigen by human T cell Clones: a model of T-T interaction. Eur. J. Immunol. 33, 315.
17. POLLACK A. (1992) Flow cytometric cell kinetic analysis by simultaneously staining nuclei with propidium iodide and fluorescein isothiocyanate. Meth. Cell Biology 33, 315.
18. PROSPERI E, GIANGARÈ MC, BOTTIROLI G. (1991) Nuclease-induced DNA structural changes assessed by flow cytometry with the intercalating dye propidium iodide. Cytometry 12, 323.
19. THORNTHWAITE JT, SUGARBAKER EV, TEMPLE WJ. (1980) Preparation of tissues for DNA flow ctyometric analysis. Cytometry 1, 229.
20. WEISBROD S. (1982) Active chromatine. Nature 287, 289.
21. WIJSMAN JH, JONKER RR, KEIJZER R, VAN DE VELDE CJH, CORNELISSE CJ, VAN DIERENDOCK JH. (1993) A new method to detect apoptosis in paraffin sections: In situ end labeling of fragmented DNA. J. Histochem. Cytochem. 41, 7.
22. WYLLIE AH. (1980) Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonucleases activation. Nature 245, 555.
23. + ATP-ase two-dimensional crystallization. Toxicol. Appl. Pharmacol. 96, 393.