Asp-49 is not an absolute prerequisite for the enzymic activity of low-Mr phospholipases A2: Purification, characterization and computer modelling of an enzymically active Ser-49 phospholipase A2, ecarpholin S, from the venom of Echis carinatus sochureki (saw-scaled viper)

Biochemical Journal

Volumn 319, Issue 3, 1996, Pages 961-968

  Polgár, János ^a   Magnenat, Edith M ^b   Peitsch, Manuel C ^b   Wells, Timothy N C ^b   Clemetson, Kenneth J ^a  

^a UNIVERSITY OF BERN   (Switzerland)

^b GLAXO INST FOR MOLECULAR BIOLOGY   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

ASPARTIC ACID; CALCIUM BINDING PROTEIN; LYSINE; PHOSPHOLIPASE A2; SERINE; SNAKE VENOM;

AMINO ACID SUBSTITUTION; ARTICLE; COMPUTER MODEL; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME PURIFICATION; NONHUMAN; PRIORITY JOURNAL; SNAKE;

AMINO ACID SEQUENCE; ANIMALS; ASPARTIC ACID; BINDING SITES; COMPUTER SIMULATION; HUMANS; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PHOSPHOLIPASES A; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SERINE; VIPER VENOMS; VIPERIDAE;

ECHIS; ECHIS CARINATUS SOCHUREKI; GLYCINE MAX; SERPENTES; VIPER;

EID: 0029961889     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/bj3190961     Document Type: Article

References (52)

1. Gelb, M. H., Jain, M. K., Hanel, A. M. and Berg, O. G. (1995) Annu. Rev. Biochem. 64, 653-688
2. Dennis, E. A. (1994) J. Biol. Chem. 269, 13057-13060
3. Dijkstra, B. W., Kalk, K. H., Hol, W. G. J. and Drenth, J. (1981) J. Mol. Biol. 147, 97-123
4. Scott, D. L., White, S. P., Otwinowski, Z., Yuan, W., Gelb, M. H. and Sigler, P. B. (1990) Science 250, 1541-1546
5. White, S. P., Scott, D. L., Otwinowski, Z., Gelb, M. H. and Sigler, P. B. (1990) Science 250, 1560-1563
6. Davidson, F. F. and Dennis, E. A. (1990) J. Mol. Evol. 31, 228-238
7. Renetseder, R., Brunie, S., Dijkstra, B. W., Drenth, J. and Sigler, P. B. (1985) J. Biol. Chem. 260, 11627-11634
8. Maraganore J. M., Merutka, G., Cho, W., Welches, W., Kézdy, F. J. and Heinrikson, R. L. (1984) J. Biol. Chem. 259, 13839-13843
9. Maraganore, J. M. and Heinrikson, R. L. (1986) J. Biol. Chem. 261, 4797-4804
10. Yoshizumi, K., Liu, S.-Y., Miyata, T., Saita, S., Ohno, M., Iwanaga, S. and Kihara, H. (1990) Toxicon 28, 43-54
11. Liu, S.-Y., Yoshizumi, K., Oda, N., Ohno, M., Tokunaga, F., Ivranaga, S. and Kihara, H. (1990) J. Biochem. (Tokyo) 107, 400-408
12. Francis, B., Gutierrez, J. M., Lomonte, B. and Kaiser, I. I. (1991) Arch. Biochem. Biophys. 284, 352-359
13. van den Bergh, C. J., Slotboom, A. J., Verheij, H. M. and de Haas, G. (1988) Eur. J. Biochem. 176, 353-357
14. Li, Y., Yu, B.-Z., Zhu, H., Jain, M. K. and Tsai, M.-D. (1994) Biochemistry 33, 14714-14722
15. Holland, D. R., Clancy, L. L., Muchmore, S. W., Ryde, T. J., Einsphar, H. M., Finzel, B. C., Heinrikson, R. L. and Watenpaugh, K. D. (1990) J. Biol. Chem. 265, 17649-17656
16. Scott, D. L. Achari, A., Vidal, J. C. and Sigler, P. B. (1992) J. Biol. Chem. 267, 22645-22657
17. Krizaj, I., Bieber, A. L., Ritonja, A. and Gubensek, F. (1991) Eur. J. Biochem. 202, 1165-1168
18. Thouin, L. G., Ritonja, A., Gubensek, F. and Russell, F. E. (1982) Toxicon 20, 1051-1058
19. Liu, C.-S., Kuo, P.-Y., Chen, J.-M., Chen, S.-W., Chang, C.-H., Tseng, C.-C., Tzeng, M.-C. and Lo, T.-B. (1992) J. Biochem. (Tokyo) 112, 707-713
20. Laemmli, U. K. (1970) Nature (London) 227, 680-685
21. Morrissey, J. H. (1981) Anal. Biochem. 117, 307-310
22. Zucker, M. B. (1989) Methods Enzymol. 169, 117-133
23. Kramer, R. M., Heissom, C., Johansen, B., Hayes, G., McGray, P., Chow, E. P., Tizard, R. and Pepinsky, R. B. (1989) J. Biol. Chem. 264, 5768-5775
24. Nieuwenhuizen, W., Kunze, H. and de Haas, G. H. (1974) Methods Enzymol. 32, 147-154
25. de Araujo, A. L. and Radvanyi, F. (1987) Toxicon 25, 1181-1188
26. Peitsch, M. C. (1995) Bio/Technology 13, 658-660
27. Peitsch, M. C. (1996) Biochem. Soc. Trans. 24, 274-279
28. Pearson, W. R. and Lipman. D. J. (1988) Proc. Natl. Acad. Sci. U.S.A. 85, 2444-2448
29. Arni, R. K., Ward, R. J., Gutierrez, J. M. and Tulinsky, A. (1995) Acta Crystallogr. D 51, 311-317
30. Luethy, R., Bowie, J. U. and Eisenberg, D. (1992) Nature (London) 356, 83-85
31. Sippl, M. J. (1993) Proteins 17, 355-362
32. de Araujo, H. S. S., White, S. P. and Ownby, C. L. (1996) Arch. Biochem. Biophys. 326, 21-30
33. Cintra, A. C. O., Marangoni, S., Oliveira, B. and Giglio, J. R. (1993) J. Protein Chem. 12, 57-64
34. Liu, C.-S., Chen, J.-M., Chang, C.-H., Chen, S.-W., Teng, C.-M. and Tsai, I.-H. (1991) Biochim. Biophys. Acta 1077, 362-370
35. Nakai, M., Nakashima, K., Ogawa, T., Shimohigashi, Y., Hattori, S., Chang, C.-C. and Ohno, M. (1995) Toxicon 33, 1469-1478
36. Zhu, H., Dupureur, C. M., Zhang, X. and Tsai, M.-D. (1995) Biochemistry 34, 15307-15314
37. Shimohigashi, Y., Tani, A., Matsumoto, H., Nakashima, K., Yamaguchi, Y., Oda, N., Takano, Y., Kamiya, H., Kishino, J., Arita, H. and Ohno, M. (1995) J. Biochem. (Tokyo) 118, 1037-1044
38. Kini, R. M. and Evans, H. J. (1989) Toxicon 27, 613-635
39. Vadas, P., Browning, J., Edelson, J. and Pruzanski, W. (1993) J. Lipid Mediators 8, 1-30
40. Lambeau, G., Barhanin, J., Schweiz, H., Qar, J. and Lazdunski, M. (1989) J. Biol. Chem. 264, 11503-11510
41. Lambeau, G., Schmid-Aliana, A., Lazdunski, M. and Barhanin, J. (1990) J. Biol. Chem. 265, 9526-9532
42. Lambeau, G., Ancian, P., Barhanin, J. and Lazdunski, M. (1994) J. Biol. Chem. 269 1575-1578
43. Arita, H., Hanasaki, K., Nakano, T., Oka, S., Teraoka, H. and Matsumoto, K. (1991) J. Biol. Chem. 266, 19139-19141
44. Parcej, D. N. and Dolly, J. O. (1989) Biochem. J. 257, 699-903
45. Krizaj, I., Dolly, J. O. and Gubensek, F. (1994) Biochemistry 33, 13938-13945
46. Lambeau, G., Ancian, P., Nicolas, J.-P., Beiboer, S. H. W., Moinier, D., Verheij, H. and Lazdunski, M. (1995) J. Biol. Chem. 270, 5534-5540
47. Ishizaki, J., Hanasaki, K., Higashino, K., Kishino, J., Kikuchi, N., Ohara, O. and Arita, H. (1994) J. Biol. Chem. 269, 5897-5904
48. Lennartz, M. R., Wileman, T. E. and Stahl, P. D. (1987) Biochem. J. 245, 705-711
49. Taylor, M. E., Conary, J. T., Lennartz, M. R., Stahl, P. D. and Drickamer, K. (1990) J. Biol. Chem. 265, 12156-12162
50. Chu, S.-T., Chu, C.-C., Tseng, C.-C. and Chen, Y.-H. (1993) Biochem. J. 295, 713-718
51. Diaz, C., Alape, A., Lomonte, B., Olamendi, T. and Gutiérrez, J. M. (1994) Arch. Biochem. Biophys. 312, 336-339
52. Pedersen, J. Z., Lomonte, B., Massoud, R., Gubensek, F., Gutiérrez, J. M. and Rufini, S. (1995) Biochemistry 34, 4670-4675