Multi-well ELISA based on independent peptide antigens for antibody capture. Application to Lyme disease serodiagnosis

Journal of Immunological Methods

Volumn 198, Issue 1, 1996, Pages 25-33

  Yu, Zhiguang ^a,b   Carter, John Mark ^c   Sigal, Leonard H ^d   Stein, Stanley ^a,b,e  

^a RUTGERS UNIVERSITY   (United States)

^b RUTGERS UNIVERSITY   (United States)

^c Cytogen Corporation   (United States)

^d ROBERT WOOD JOHNSON MEDICAL SCHOOL   (United States)

^e CABM   (United States)

Author keywords

ELISA; Epitope; Lyme disease; Peptide antigen

Indexed keywords

EPITOPE; PARASITE ANTIGEN; SYNTHETIC PEPTIDE;

ARTICLE; BORRELIA BURGDORFERI; CLINICAL ARTICLE; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; LYME DISEASE; PRIORITY JOURNAL; SERODIAGNOSIS;

BACTERIA (MICROORGANISMS); BORRELIA; BORRELIA BURGDORFERI; BOVINAE;

EID: 0029961377     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/0022-1759(96)00140-8     Document Type: Article

References (20)

1. Barbour, A.G. and Fish, D. (1993) The biological and social phenomenon of Lyme disease. Science 260, 1610-1616.
2. Bergstrom, S., Bundoc, V.G. and Barbour, A.G. (1989) Molecular analysis of linear plasmid-encoded major surface proteins, OspA and OspB, of the Lyme disease spriochaete Borrelia burgdorferi. Mol. Microbiol. 3, 479-486.
3. Bianchi, E., Folgori, A., Wallace, A., Nicotra, M., Acali, S., Phalipon, A., Barbato, G., Bazzo, R., Cortese, R., Falici, F. and Pessi, A. (1995) A conformationally homogeneous combinatorial peptide library. J. Mol. Biol. 247, 154-160.
4. Bundoc, V.G. and Barbour, A.G. (1989) Clonal polymorphisms of outer membrane protein OspB of Borrelia burgdorferi. Infect. Immun. 57, 2733-2741.
5. Burgdorfer, W., Barbour, A.G., Hayes, S.F., Benach, J.L., Grunwaldt, E. and Davis, J.P. (1982) Lyme Disease-A tick-borne spirochetosis? Science 216, 1317-1319.
6. Coleman, J.L. and Benach, J.L. (1992) Characterization of antigenie determinants of Borrelia burgdorferi shared by other bacteria. J. Infec. Dis. 165, 658-666.
7. Dressler, F., Whalen, J.A., Reinhardt, B.N. and Steere, A.C. (1993) Western blotting in the serodiagnosis of Lyme disease. J. Infect. Dis. 167, 392-400.
8. Fikrig, E., Berland, R., Chen, M., Williams, S., Sigal, L.H. and Flavell, R.A. (1993) Serological response to the Borrelia burgdorferi flagellin demonstrates an epitope common to a neuroblastoma cell line. Proc. Natl. Acad. Sci. USA 90, 183-187.
9. Geysen, H.M., Meloen, R.H. and Barteling, S.J. (1984) Use of peptide synthesis to probe viral antigens for epitopes to a resolution of a single amino acid. Proc. Natl. Acad. Sci. USA 81, 3998-4002.
10. Jauris-Heipke, S., Fuchs, R., Motz, M., Preac-Mursic, V., Schwab, E., Soutschek, E., Will, G. and Wilske, B. (1993) Genetic heterogeneity of the genes coding for the outer surface protein C (OspC) and the flagellin of Borrelia burgdorferi. Med. Microbiol. Immunol. 182, 37-50.
11. Magnarelli, L.A., Miller, J.N., Anderson, J.F. and Riviere, G.R. (1990) Cross-reactivity of nonspecific treponemal antibody in serological tests for Lyme disease. J. Clin. Microbiol. 28, 1276-1279.
12. Perng, G., Lefebvre, R.B. and Johnson, R.C. (1991) Further characterization of a potent immunogen and the chromosomal gene encoding it in the Lyme disease agent. Infect. Immun. 59, 2070-2074.
13. Riddles, P.W., Blakeley, R.L. and Zerner, B. (1979) Ellman's reagent: 5,5′-Dithiobis(2-nitrobenzoic acid) - a reexamination. Anal. Biochem. 94, 75-81.
14. Schneider, T., Lange, R., Ronspeck, W., Weigelt, W. and Kolmel, H.W. (1992) Prognostic B-cell epitopes on the flagellar protein of Borrelia burgdorferi. Infect. Immun. 60, 316-319.
15. Schutzer, S.E., Coyle, P.K., Dunn, J.J., Luft, B.J. and Brunner, M. (1994) Early and specific antibody response to OspA in Lyme disease. J. Clin. Invest. 94, 454-457.
16. Schwan, T.G., Karstens, R.H., Schrumpf, M.E. and Simpson, W.E. (1991) Changes in antigenjc reactivity of Borrelia burgdorferi, the Lyme disease spirochete, during persistent infection in mice. Can. J. Microbiol. 37, 450-454.
17. Valero, M., Camarero, J.A., Adeva, A., Verdaguer, N., Fita, I., Mateu, M.G., Domingo, E., Giralt, E. and Andreu, D. (1995) Cyclic peptides as conformationally restricted models of viral antigens: Application to foot and mouth disease virus. Biochem. Peptides Prot. Nucleic Acids 1, 133-140.
18. Van Dam, A.P., Kuiper, H., Vos, K., Widjojokusumo, A., de Jongh, B.M., Spanjaard, L., Ramsclaar, A.C.P., Kramer, M.D. and Dankert, J. (1993) Different genospecies of Borrelia burgdorferi are associated with distinct clinical manifestations of Lyme borreliosis. Clin. Infect. Dis. 17, 708-717.
19. Yu, Z., Carter, J.M., Huang, S.-Y., Lackland, H., Sigal, L.H. and Stein, S. (1996) Presentation of peptide antigens as albumin conjugates for use in detection of serum antibodies by ELISA. Bioconj. Chem. 7, 338-342.
20. Zoller, L., Burkard, S. and Schafer, H. (1991) Validity of Western immunoblot band patterns in the serodiagnosis of Lyme borreliosis. J. Clin. Microbiol. 2, 174-182.