Constitutive in vivo mRNA expression by osteocytes of β-actin, osteocalcin, connexin-43, IGF-I, c-fos and c-jun, but not TNF-α nor tartrate-resistant acid phosphatase

Journal of Bone and Mineral Research

Volumn 11, Issue 3, 1996, Pages 350-357

  Mason, Deborah J ^a   Hillam, Richard A ^a   Skerry, Timothy M ^b  

^a UNIVERSITY OF BRISTOL   (United Kingdom)

^b UNIVERSITY OF YORK   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ACID PHOSPHATASE; BETA ACTIN; CONNEXIN 43; SOMATOMEDIN C; TUMOR NECROSIS FACTOR ALPHA;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BONE MASS; CORTICAL BONE; GENE EXPRESSION; GENE EXPRESSION REGULATION; NONHUMAN; ONCOGENE C FOS; OSSIFICATION; OSTEOCYTE; PERIOSTEUM; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

EID: 0029960225     PISSN: 08840431     EISSN: None     Source Type: Journal    
DOI: 10.1002/jbmr.5650110308     Document Type: Article

References (47)

1. Rodan GA 1991 Mechanical loading, estrogen deficiency and the coupling of bone formation to bone resorption. J Bone Miner Res 6:527-530.
2. Smith EL, Giliigan C 1991 Physical-activity effects on bone metabolism. Calcif Tissue Int 49:550-554.
3. Smith EJ, Smith PE, Ensign a, Shea MM 1984 Bone involution decrease in exercising middle-aged women. Calcif Tissue Int 36 (Suppl 1):129-138.
4. Ramsdale SJ, Bassey EJ 1993 Changes in femoral bone mineral density associated with impact exercise in postmenopausal women. J Physiol 467:313P.
5. Bassey EJ. Ramsdale SJ 1994 Increase in femoral bone density in young women following high-impact exercise. Osteoporosis Int 4:72-75.
6. Ming Z, Zaman G, Lanyon LE 1993 Estrogen enhances the osteogenic effects of mechanical loading and exogenous prostacyclin, but not prostaglandin-E2. J Bone Miner Res 8 (Suppl 1):151.
7. Ming Z, Zaman G, Lanyon LE 1994 Estrogen enhances the stimulation of bone collagen synthesis by loading and exogenous prostacyclin, but not prostaglandin E2, in organ cultures of rat ulnae. J Bone Miner Res 9:805-816.
8. Vaes G 1988 Cellular biology and biochemical mechanism of bone resorption. A review of recent developments on the formation, activation, and mode of action of osteoclasts. Clin Orthopaed 231:239-271.
9. Kummer B1962 Funktioneller bau und funktionelle anpassung des knochens. Anat Anz Bd 110:3261-5293.
10. Lanyon LE 1993 Osteocytes, strain detection, bone modeling and remodeling. Calcif Tissue Int 53 (Suppl 1):102-107.
11. Skerry TM, Bitensky L, Chayen J, Lanyon LE 1989 Early strain-related changes in enzyme activity in osteocytes following bone loading in vivo. J Bone Miner Res 4:783-788.
12. Pead MJ, Suswillo RFL, Skerry TM, Vedi S, Lanyon LE 1988 Increased 3H-uridine levels in osteocytes following a single short period of dynamic bone loading in vivo. Calcif Tissue Int 43:92-96.
13. Lean JM, Jagger CJ, Chambers TJ, Chow JWM 1995 Increased insulin-like growth factor I mRNA expression in rat osteocytes in response to mechanical stimulation. Am J Physiol Endocrinol Metabol 268:318-327.
14. Jones SJ, Gray C, Sakamaki H, Arora M, Boyde A, Gourdie R, Green C 1993 The incidence and size of gap-junctions between the bone-cells in rat calvaria. Anat Embryol 187:343-352.
15. Dory SB 1981 Morphological evidence of gap junctions between bone cells. Calcif Tissue Int 33:509-512.
16. Doty SB. Morey Holton ER 1982 Changes in osteoblastic activity due to simulated weightless conditions. Physiologist 25:S141-S142.
17. Braidman IP, Davenport LK, Carter DH, Selby PL, Mawer EB, Freemont AJ 1995 Preliminary in situ identification of estrogen target cells in bone. J Bone Miner Res 10(1):74-81.
18. Fermor B, Skerry TM 1995 PTH/PTHrP receptor expression on osteoblasts and osteocytes but not resorbing bone surfaces in growing rats. J Bone Miner Res 10:1935-1943.
19. van der Plas A, Aarden EM, Feijen JHM, de Boer AH, Wiltink A. Alblas MJ. de Leij L, Nijweide PJ 1994 Characteristics and properties of osteocytes in culture. J Bone Miner Res 9(11): 1697-1704.
20. Hughes DE, Salter DM. Simpson R 1994 Cd44 expression in human bone: a novel marker of osteocytic differentiation. J Bone Miner Res 9:39-44.
21. Hughes DE, Salter DM. Dedhar S. Simpson R 1993 Integrin expression in human bone. J Bone Miner Res 8:527-533.
22. Weinreb M, Shinar D, Rodan GA 1990 Different pattern of alkaline phosphatase. osteopontin, and osteocalcin expression in developing rat bone visualized by in situ hybridization. J Bone Miner Res 5:831-842.
23. Birch MA, Ginty AF. Walsh CA, Fraser WD, Gallagher JA, Bilbe G 1993 PCR detection of cytokines in normal human and pagetic osteoblast-like cells. J Bone Miner Res 8:1155-1162.
24. Birch MA, Taylor W, Fraser WD. Ralston SH. Hart CA, Gallagher JA 1994 Absence of paramyxovirus RNA in cultures of pagetic bone cells and in pagetic bone. J Bone Miner Res 9:11-16.
25. Ralston SH 1994 Analysis of gene expression in human bone biopsies by polymerase chain reaction: Evidence for enhanced cytokine expression in postmenopausal osteoporosis. J Bone Miner Res 9:883-890.
26. Araki N, Robinson FD, Nishimoto SK 1993 Rapid and sensitive method for quantitation of bone gla protein mRNA using competitive polymerase chain reaction. J Bone Miner Res 8:313-322.
27. Hillam RA, Skerry TM 1995 Inhibition of bone resorption and stimulation of formation by mechanical loading of the modeling rat ulna in vivo. J Bone Miner Res 105:683-689.
28. Torrance AG. Mosley JM, Suswillo RFL, Lanyon LE 1994 Noninvasive loading of the rat ulna in vivo induces a strain related modeling response uncomplicated by trauma of periosteal pressure. Calcif Tissue Int 54:(3):241-247.
29. Chomczynski P, Sacchi N 1987 Single step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Ann Biochem 162:156-159.
30. Sambrook J, Maniatis T, Fritsch EF 1989 Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Press, Cold Spring Harbor, New York.
31. Celeste AJ, Rosen V, Buecker JL, Kriz R, Wang EA. Wozney JM 1986 Isolation of the human gene for bone gla protein utilizing mouse and rat cDNA clones. EMBO J 5:1885-1890.
32. Shirai T, Shimizu N, Horiguchi S, Ito H 1989 Cloning and expression in E. coli of the gene for rat TNF. Agric Biol Chem 53:1733-1736.
33. Curran T, Gordon MB, Rubino KL. Sambucetti LC 1987 Isolation and characterisation of the c-fos (rat) cDNA and analysis of post-translational modification in vitro. Oncogenc 2:79-84.
34. Beyer EC. Paul DL, Goodenough DA 1987 Connexin 43: A protein from rat heart homologous to a gap junction protein from liver. J Cell Biol 105:2621-2629.
35. Ek-Rylander B, Bill P, Norgaad M, Nilsson S, Andersson G 1992 Cloning, sequence and developmental expression of a Type 5 TRAP of rat bone. J Biol Chem 266:24684-24689.
36. Sullivan R, Ruangvoravat C, Joo D, Morgan J, Wang BL, Wang XK, Lo CW 1993 Structure, sequence and expression of the mouse Cx43 gene encoding connexin-43. Gene 130:191-199.
37. Reddy SV, Scarcez T, Windle JJ, Leach RJ, Hundley JE, Chirgwin JM, Chou JY, Roodman GD 1993 Cloning and characterization of the 5′-flanking region of the mouse tartrateresistant acid phosphatase gene. J Bone Miner Res 8:1263-1270.
38. Nishida T. Nishino N, Takano M, Sekiguchi Y, Kawai K, Mizuno K. Nakai S. Masui Y, Hirai Y 1989 Molecular cloning and expression of rat IL-1alpha cDNA. J Biochem 105:351-357.
39. Kitabayashi I, Saka F, Gachelin G, Yokoyama K 1990 Nucleotide sequence of rat c-jun proto-oncogene. Nucleic Acids Res 18:3400.
40. Boden SD, Joyce ME. Oliver B, Heydemann A. Bolander ME 1989 Estrogen receptor mRNA expression in callus during fracture healing in the rat. Calcif Tissue Int 45:324-325.
41. Laird DW, Puranam KL, Revel JP 1991 Turnover and phosphorylation dynamics of connexin 43 gap junction protein in cultured cardiac myocytes. Biochem J 273:67-72.
42. Husy T, Mikalsen SO, Sanner T 1993 Phosphatase inhibitors, gap junctional intercellular communication and (12 5i)-EGF binding in hamster fibroblasts. Carcinogenesis 14:2257-2265.
43. Skerry TM, Gowen M, Dodds RA 1993 Formation and resorption in the modeling rat ulna. Bone 15(4):454.
44. Wang Z-Q, Ovitt C, Grigoriadis AE, Möhle-Steinlein U, Rüther U, Wagner EF 1992 Bone and haematopoietic defects in mice lacking c-fos. Nature 360:741-745.
45. Grigoriadis AE, Schellander K, Wang ZQ, Wagner EF 1993 Osteoblasts are target-cells for transformation in c-fos transgenic mice. J Cell Biol 122:685-701.
46. Middleton J, Arnott N, Walsh CA, Beresford JN 1995 Osteoblasts and osteoclasts in adult human osteophyte tissue express the mRNAs for IGFI and II and the type IIGF receptor. Bone 16(3):287-293.
47. Liang P. Pardee AB 1992 Differential display of eukaryotic mRNA by means of the polymerase chain-reaction. Science 257:967-971.