Expression of protamine P2 in the testis of the common marmoset and man visualized using non-radioactive in-situ hybridization

International Journal of Andrology

Volumn 19, Issue 4, 1996, Pages 212-219

  Saunders, P T K ^a   Gaughan, J ^a   Saxty, B A ^a   Kerr, L E ^a   Millar, M R ^a  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

Digoxigenin; In situ hybridization; Nuclear condensation; Protamine; Spermatid

Indexed keywords

MESSENGER RNA; PROTAMINE;

ANIMAL TISSUE; ARTICLE; CELL DIFFERENTIATION; CONTROLLED STUDY; GERM CELL; HUMAN; HUMAN TISSUE; IN SITU HYBRIDIZATION; MALE; MARMOSET; NONHUMAN; NORTHERN BLOTTING; PRIMATE; PRIORITY JOURNAL; PROTEIN EXPRESSION; SEMINIFEROUS TUBULE EPITHELIUM; SPERMATID; SPERMATOGENESIS; STAINING; TESTIS;

EID: 0029958136     PISSN: 01056263     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2605.1996.tb00465.x     Document Type: Article

References (44)

1. Altschul, S., Gish, W., Miller, W., Myers, E. & Lipman, D. (1990) Basic local alignment search tool. Journal of Molecular Biology, 215, 403-410.
2. Bellve, A., McKay, D., Renaux, B. & Dixon, G. (1988) Purification and characterisation of mouse protamines P1 and P2. Amino acid sequence of P2. Biochemistry, 27, 2890-2897.
3. Belokopytova, I., Kostyleva, E., Tomilin, A. & Vorob'ev, V. (1993) Human infertility may be due to a decrease of the protamine P2 content in sperm chromatin. Molecular Reproduction and Development, 34, 53-57.
4. Braun, R., Peschon, J., Behringer, R., Brinster, R. & Palmiter, R. (1989) Protamine 3′-untranslated sequences regulate temporal translational control and subcellular localizaton of growth hormone in spermatids of transgenic mice. Genes and Development, 3, 793-802.
5. Busuttil, A., Orr, S. & Hargreave, T. (1983) Histopathology. In Male Infertility (ed. T. Hargreave), pp. 112-143. Springer-Verlag, Berlin.
6. Chomczynski, P. & Sacchi, N. (1987) Single step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry, 162, 156-159.
7. Chowdhury, A. K. & Steinberger, E. (1976) A study of germ cell morphology and duration of spermatogenic cycle in the baboon, Papio anubis. Anatomical Record, 185, 155-169.
8. Clermont, Y. (1963) The cycle of the seminiferous epithelium in man. American Journal of Anatomy, 112, 35-51.
9. Clermont, Y. & Trott, M. (1969) Duration of the cycle of the seminiferous epithelium in the mouse and hamster determined by means of 3H-thymidine and radioautography. Fertility and Sterility, 20, 805-817.
10. Davis, L. G., Dibner, M. D. & Battey, J. F. (1986) Basic Methods in Molecular Biology. Elsevier, Inc., New York.
11. De Rooij, D. G., Van Alphen, M. M. A. & Van De Kant, H. J. G. (1986) Duration of the cycle of the seminiferous epithelium and its stages in the rhesus monkey (Macaca mulatta). Biology of Reproduction, 35, 587-591.
12. Domenjoud, L., Nussbaum, G., Adham, I., Greeske, G. & Engel, W. (1990) Genomic sequences of human protamines whose genes, PRM1 and PRM2 are clustered. Genomics, 8, 127-133.
13. Eidelsberg, J., Bruger, M. & Lipkin, M. (1942) Some anabolic effects of testosterone implants. Journal of Clinical Endocrinology, 2, 329-331.
14. Einspanier, A., Ivell, R., Rune, G. & Hodges, J. (1994) Oxytocin gene expression and oxytocin immunoactivity in the ovary of the common marmoset monkey (Callithrix jacchus). Biology of Reproduction, 50, 1216-1222.
15. Foresta, C., Zorzi, M., Rossato, M. & Varotto, A. (1992) Sperm nuclear instability and staining with aniline blue: abnormal persistance of histones in spermatozoa in infertile men. International Journal of Andrology, 15, 330-337.
16. Haider, S. G., Passia, D., Treiber, A. & Milhorst, S. (1989) Description of eight phases of spermiogenesis in the marmoset testis. Acta Anatomica, 135, 180-184.
17. Hecht, N. B. (1990) Regulation of 'haploid expressed genes' in male germ cells. Journal of Reproduction and Fertility, 88, 679-693.
18. Heidaran, M. A., Showman, R. M. & Kistler, W. S. (1988) A cytochemical study of the transcriptional and translational regulation of nuclear transition protein (TP-1), a major chromosomal protein of mammalian spermatids. Journal of Cell Biology, 106, 1427-1433.
19. Kleene, K. C. (1989) Poly (A) shortening accompanies the activation of translation of five mRNA's during spermiogenesis in the mouse. Development, 106, 367-373.
20. Kwon, Y. & Hecht, N. B. (1993) Binding of a phosphoprotein to the 3′ untranslated region of the mouse protamine 2 mRNA temporally represses its translation. Molecular and Cellular Biology, 13, 6547-6557.
21. Leblond, C. P. & Clermont, Y. (1952) Definition of the stages of the cycle of the seminiferous epithelium in the rat. Annals of the New York Academy of Sciences, 55, 548-573.
22. Lee, M. M., Gustafson, M. L., Ukiyama, E., Donahoe, P. K., MacLaughlin, D. T., Wexler, M. & Keeping, H. S. (1994) Developmental changes in mullerian inhibiting substance in the cynomologous monkey, Macaca fasicularis. Journal of Clinical Endocrinology and Metabolism, 78, 615-621.
23. Majdic, G., Millar, M. R. & Saunders, P. T. K. (1995) Immunolocalisation of androgen receptors to interstitial cells in fetal rat testes and to mesenchymal and epithelial cells of associated ducts. Journal of Endocrinology, 147, 285-293.
24. Mali, P., Kaipia, A., Kangasniemi, M., Toppari, J., Sandberg, M., Hecht, N. B. & Parvinen, M. (1989) Stage-specific expression of nucleoprotein mRNAs during rat and mouse spermiogenesis. Reproduction, Fertility and Development, 1, 369-382.
25. Millar, M. R., Sharpe, R. M., Maguire, S. M., Gaughan, J. & Saunders, P. T. K. (1995) Co-localisation of mRNA and protein using in-situ hybridization and immunocytochemistry in testicular tissue. Microscopy Research and Technique, 32, 498-503.
26. Millar, M. R, Sharpe, R. M., Maguire, S. M. & Saunders, P. T. K. (1993) Cellular localisation of messenger RNAs in rat testis; application of digoxigenin-labelled probes to embedded tissue. Cell and Tissue Research, 273, 269-277.
27. O'Bryan, M., Mallidis, C., Murphy, B. & Baker, H. M. G. (1994) Immunohistochemical localization of clusterin in the male genital tract in humans and marmosets. Biology of Reproduction, 50, 502-509.
28. Paterson, M., Koothan, P., Morris, K., O'Byrne, K., Braude, P., Williams, A. & Aitken, R. J. (1992) Analysis of the contraceptive potential of antibodies against native and deglycosylated porcine ZP3 in vivo and in vitro. Biology of Reproduction, 46, 523-534.
29. Retief, J. & Dixon, G. (1993) Evolution of pro-protamine P2 genes in primates. European Journal of Biochemistry, 214, 609-615.
30. Retief, J., Winkfein, R., Dixon, G., Adroer, R., Queralt, R., Ballabriga, J. & Oliva, R. (1993) Evolution of protamine P1 genes in primates. Journal of Molecular Evolution, 37, 426-434.
31. Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B. & Erich, H. A. (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science, 239, 224-230.
32. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989) Molecular cloning. A Laboratory Manual, 2nd edn. Cold Spring Harbor Press, New York.
33. Saunders, P. T. K., McKinnell, C. M., Millar, M. R., Gaughan, J., Turner, K., J. Jegou, B., Syed, V. & Sharpe, R. M. (1995) Phosphatidylethanolamine binding protein is an abundant secretory product of haploid testicular germ cells in the rat. Molecular and Cellular Endocrinology, 107, 221-230.
34. Saunders, P. T. K., Millar, M. R., Majdic, G., McLaren, T. T., Bremner, W. B., Grigor, K. & Sharpe, R. M. (1996) Testicular androgen receptor protein: distribution and control of expression. In Cellular and Molecular Regulation of Testicular Cells (ed. C. Desjardins). Springer Verlag, Berlin, in press.
35. Saunders, P. T. K., Summers, P. M. & Hearn, J. (1987) Partial purification and characterization of chorionic gonadotrophin in plasma and in culture medium of trophoblast cells from the marmoset monkey (Callitrix jacchus). Journal of Reproduction and Fertility, 80, 463-472.
36. Saunders, P. T. K., Millar, M. R., Maguire, S. M. & Sharpe, R. M. (1992) Stage-specific expression of rat transition protein-2 mRNA and possible localization to the chromatoid body of step 7 spermatids by in situ hybridization using a nonradioactive riboprobe. Molecular Reproduction and Development, 33, 385-391.
37. Saunders, P. T. K., Millar, M. R. & Sharpe, R. M. (1993) Mitochondrial cytochrome C oxidase is expressed at high levels in a stage dependent manner in pachytene spermatocytes during spermatogenesis in the rat. Biology of Reproduction, 48, 57-67.
38. Schlatt, S. & Weinbauer, G. (1994) Immunohistochemical localization of proliferating nuclear cell antigen as a tool to study cell proliferation in rodent and primate testes. International Journal of Andrology, 17, 214-222.
39. Schulze, W. & Rehder, U. (1984) Organization and morphogenesis of the human seminiferous epithelium. Cell and Tissue Research, 237, 395-407.
40. Schulze, W., Riemer, M., Rehder, U. & Hohne, K. H. (1986) Computer-aided three-dimensional reconstructions of the arrangement of primary spermatocytes in human seminiferous tubules. Cell and Tissue Research, 244, 1-8.
41. Tanhauser, S. & Hecht, N. B (1989) Nucleotide sequence of the rat protamine 2 gene. Nucleic Acids Research, 17, 4395.
42. Tardif, S. & Jaqish, C. (1994) The common marmoset as a model for nutritional impacts upon reproduction. Annals of the New York Academy of Sciences, 709, 214-215.
43. Ward, S. W. & Coffey, D. S. (1991) DNA packaging and organization in mammalian spermatozoa: comparison with somatic cells. Biology of Reproduction, 44, 569-574.
44. Wilton, L., Marshall, V., Piercy, E. & Moore, H. (1993) In vitro fertilization and embryo development in the marmoset monkey (Callithrix jacchus). Journal of Reproduction and Fertility, 97, 481-486.