The hemagglutinin genes hagB and hagC of Porphyromonas gingivalis are transcribed in vivo as shown by use of a new expression vector

Infection and Immunity

Volumn 64, Issue 11, 1996, Pages 4802-4810

  Lee, Seok Woo ^a   Hillman, Jeffrey D ^a   Progulske Fox, Ann ^a  

^a UNIVERSITY OF FLORIDA COLLEGE OF DENTISTRY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GALACTOKINASE; GENE PRODUCT; HEMAGGLUTININ; TETRACYCLINE;

ABSCESS; ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENE; CLONING VECTOR; ENZYME ACTIVITY; EXPRESSION VECTOR; FEMALE; GENE ISOLATION; MOUSE; NONHUMAN; PERIODONTAL DISEASE; PORPHYROMONAS; PRIORITY JOURNAL; SEQUENCE ANALYSIS;

EID: 0029957172     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/iai.64.11.4802-4810.1996     Document Type: Article

References (43)

1. Adams, C. W., and G. W. Halfield. 1984. Effects of promoter strengths and growth conditions on copy number of transcription-fusion vectors. J. Biol. Chem. 259:7399-7403.
2. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Strahl. 1987. Current protocols in molecular biology. Greene Publishing Associates, New York.
3. Bourgeau, G., H. Lapointe, P. Peloquin, and D. Mayrand. 1992. Cloning, expression, and sequencing of a protease gene (tpr) from Porphyromonas gingivalis W83 in Escherichia coli. Infect. Immun. 60:3186-3192.
4. Casadaban, M. J., and S. N. Cohen. 1979. Lactose genes fused to exogenous promoters in one step using a MU-lac bacteriophage: in vivo probe for transcriptional control sequences. Proc. Natl. Acad. Sci. USA 76:4530-4533.
5. Chen, P. B., M. E. Neiders, S. J. Millar, H. S. Reynolds, and J. J. Zambon. 1987. Effect of immunization on experimental Bacteroides gingivalis infection in a murine model. Infect. Immun. 55:2534-2537.
6. Dahlen, G. G. 1993. Black-pigmented gram-negative anaerobes in periodontitis. FEMS Immunol. Med. Microbiol. 6:181-192.
7. Dickinson, D. P., M. A. Kuniniec, F. Yoshimura, and R. J. Genco. 1988. Molecular cloning and sequencing of the gene encoding the fimbrial subunit protein of Bacteroides gingivalis. J. Bacteriol. 170:1658-1665.
8. DiRita, V. J., and J. J. Mekalanos. 1989. Genetic regulation of bacterial virulence. Annu. Rev. Genet. 23:455-482.
9. Dyer, D. W., G. Bilailis, J. H. Michel, and R. Malek. 1992. Conjugal transfer of plasmid and transposon DNA from Esherichia coli into Porphyromonas gingivalis. Biochem. Biophys. Res. Commun. 186:1012-1019.
10. Erlich, H. A. (ed.). 1989. PCR technology. Principles and applications for DNA amplification. M. Stockton Press, New York.
11. Finn, T. M., J. P. Arbuthnoti, and G. Dougan. 1982. Properties of Escherichia coli grown in vivo using a chamber implant system. J. Gen. Microbiol. 128:3083-3091.
12. Fletcher, H. M., H. A. Schenkein, and F. L. Macrina. 1994. Cloning and characterization of a new protease gene (prtH) from Porphyromonas gingivalis. Infect. Immun. 62:4279-4286.
13. Fletcher, H. M., H. A. Schenkein, R. M. Morgan, K. A. Bailey, C. R. Berry, and F. L. Macrina. 1995. Virulence of a Porphyromonas gingivalis W83 mutant defective in the prtH gene. Infect. Immun. 63:1521-1528.
14. Fujiwara, T., S. Morishima, I. Takahashi, and S. Hamada. 1993. Molecular cloning and sequencing of the fimbrillin gene of Porphyromonas gingivalis strains and characterization of recombinant proteins. Biochem. Biophys. Res. Commun. 30:241-247.
15. Genco, C. A., C. W. Cutler, D. Kapczynski, K. Maloney, and R. R. Arnold. 1991. A novel mouse model to study the virulence of and host response to Porphyromonas (Bacteroides) gingivalis. Infect. Immun. 59:1255-1263.
16. Guiney, D. G., P. Hasegawa, K. Bouic, and B. Matthews. 1989. Genetic transfer systems in Bacteroides: cloning and mapping of the transposable tetracycline-resistance locus. Mol. Microbiol. 3:1617-1623.
17. Holt, S. C., and T. E. Bramanti. 1991. Factors in virulence expression and their role in periodontal disease pathogenesis. Crit. Rev. Oral Biol. Med. 2:177-281.
18. Kato, T., N. Takahashi, and H. K. Kuramitsu. 1992. Sequence analysis and characterization of the Porphyromonas gingivalis prtC gene, which expresses a novel collagenase activity. J. Bacteriol. 174:3889-3895.
19. Kuratana, M., T. J. Inzana, and P. Anderson. 1989. Source of low-molecular-weight host factors that phenotypically increase the resistance of Haemophilus influenzae type b to bacteriolysis: non-identity with a factor active in gonococci. Microb. Pathog. 7:73-77.
20. Lépine, G. 1994. A molecular approach to the study of hemagglutinin genes of Porphyromonas gingivalis. Ph.D. dissertation. University of Florida, Gainesville.
21. Mahan, M. J., J. M. Slauch, and J. J. Mekalanos. 1993. Selection of bacterial virulence genes that are specifically induced in host tissues. Science 259:686-688.
22. Mahan, M. J., J. W. Tobias, J. M. Slauch, P. C. Hanna, R. J. Collier, and J. J. Mekalanos. 1995. Antibiotic-based selection for bacterial genes that are specifically induced during infection of a host. Proc. Natl. Acad. Sci. USA 92:669-673.
23. Malek, R., J. G. Fisher, A. Caleca, M. Stinson, C. J. van Oss, J.-Y. Lee, M.-I. Cho, R. J. Genco, R. T. Evans, and D. W. Dyer. 1994. Inactivation of the Porphyromonas gingivalis fimA gene blocks periodontal damage in gnotobiotic rats. J. Bacteriol. 176:1052-1059.
24. Maurelli, A. T. 1989. Temperature regulation of virulence genes in pathogenic bacteria: a general strategy for human pathogens? Microb. Pathog. 7:1-10.
25. McKee, A. S., A. S. McDermid, A. Baskerville, A. B. Dowsett, D. C. Ellwood, and P. D. Marsh. 1986. Effect of hemin on the physiology and virulence of Bacteroides gingivalis W50. Infect. Immun. 52:349-355.
26. McKenney, K., H. Shimatake, D. Court, U. Schmeissner, and M. Rosenberg. 1981. A system to study promoter and terminator signals recognized by E. coli RNA polymerase, p. 383-415. In J. Chirikjian and T. Papas (ed.), Gene amplification and analysis, vol. 2. Structural analysis of nucleic acids. Elsevier, New York.
27. Mekalanos, J. 1992. Environmental signals controlling expression of virulence determinants in bacteria. J. Bacteriol. 174:1-7.
28. Miller, J. F., J. J. Mekalanos, and S. F. Falkow. 1989. Coordinate regulation and sensory transduction in the control of bacterial virulence. Science 243:916-922.
29. Neiders, M. E., P. B. Chen, H. Suido, H. S. Reynolds, J. J. Zambon, M. Shlossman, and R. J. Genco. 1989. Heterogeneity of virulence among strains of Bacteroides gingivalis. J. Periodontal Res. 24:192-198.
30. Okuda, K., J. Slots, and R. J. Genco. 1981. Bacteroides gingivalis, Bacteroides asaccharolyticus, and Bacteroides melaninogenicus subspecies: cell surface morphology and adherence to erythrocytes and human buccal epithelial cells. Curr. Microbiol. 6:7-12.
31. Okuda, K., and I. Takazoe. 1974. Hemagglutinating activity of Bacteroides melaninogenicus. Arch. Oral Biol. 19:415-416.
32. Otogoto, J., and H. K. Kuramitsu. 1993. Isolation and characterization of the Porphyromonas gingivalis prtT gene, coding for protease activity. Infect. Immun. 61:117-123.
33. Patrick, S. 1988. Phagocytosis of Bacteroides fragilis in vivo and in vitro, p. 31-41. In J. M. Hardie and S. P. Borriello (ed.). Anaerobes today. John Wiley, London.
34. Progulske-Fox, A., S. Tumwarson, and S. C. Holt. 1989. The expression and function of Bacteroides gingivalis hemagglutinin gene in Escherichia coli. Oral Microbiol. Immunol. 4:121-125.
35. Progulske-Fox, A., S. Tumwasorn, G. Lépine, J. Whitlock, D. Savett, J. J. Ferretti, and J. A. Banas. 1995. The cloning, expression and sequence analysis of a second Porphyromonas gingivalis gene that codes for a protein involved in hemagglutination. Oral Microbiol. Immunol. 10:311-318.
36. Rosenberg, M., A. Cheplinsky, and K. McKenney. 1983. Studying promoters and terminators by gene fusion. Science 222:734-739.
37. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
38. Simon, R., U. Priefler, and A. Pühler. 1983. A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram-negative bacteria. Biotechniques 1:784-791.
39. Slauch, J. M., M. J. Mahan, and J. J. Mekalanos. 1994. Measurement of transcriptional activity in pathogenic bacteria recovered directly from infected host tissue. Biotechniques 16:641-644.
40. Slots, J., and R. J. Genco. 1984. Microbial pathogenicity. Black-pigmented Bacteroides species, Capnocytophaga species, and Actinobacillus actinomycetemcomitans in human periodontal disease: virulence factors in colonization, survival, and tissue destruction. J. Dent. Res. 63:412-421.
41. Sundqvist, G., D. Figdor, L. Hanstrom, S. Sorlin, and G. Sandstrom. 1991. Phagocytosis and virulence of different strains of Porphyromonas gingivalis. Scand. J. Dent. Res. 99:117-129.
42. Titus, D. E. 1991. Promega protocols and applications guide. Promega Corp., Madison, Wis.
43. Topoll, H. H., D. E. Lange, and R. F. Muller. 1990. Multiple periodontal abscesses after systemic antibiotic therapy. J. Clin. Periodontol. 17:268-272.