An inducible expression system of histidine-tagged proteins in Streptomyces lividans for one-step purification by Ni2+ affinity chromatography

FEMS Microbiology Letters

Volumn 137, Issue 2-3, 1996, Pages 135-140

  Enguita, Francisco J ^a   De La Fuente, Juan Luis ^a   Martín, Juan F ^a   Liras, Paloma ^a  

^a UNIVERSITY OF LEÓN   (Spain)

Author keywords

Affinity chromatography; Cephamycin biosynthesis genes; Expression vector; Protein purification

Indexed keywords

CEPHAMYCIN; HISTIDINE;

AFFINITY CHROMATOGRAPHY; ARTICLE; BIOSYNTHESIS; CONTROLLED STUDY; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE EXPRESSION REGULATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN PURIFICATION; STREPTOMYCES LIVIDANS;

BACTERIAL PROTEINS; BASE SEQUENCE; CHROMATOGRAPHY, AFFINITY; ESCHERICHIA COLI; GENE EXPRESSION; GENES, BACTERIAL; GENETIC VECTORS; HISTIDINE; KANAMYCIN KINASE; METHYLTRANSFERASES; MIXED FUNCTION OXYGENASES; MOLECULAR SEQUENCE DATA; MULTIENZYME COMPLEXES; NOCARDIA; OLIGODEOXYRIBONUCLEOTIDES; PHOSPHOTRANSFERASES (ALCOHOL GROUP ACCEPTOR); PLASMIDS; RECOMBINANT FUSION PROTEINS; STREPTOMYCES; THIOSTREPTON; TRANSFORMATION, GENETIC;

EID: 0029955593     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1574-6968.1996.tb08095.x     Document Type: Article

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