Comparison of detergent-solubilized membrane and soluble proteins from flow cytometrically sorted X- and Y-chromosome bearing porcine spermatozoa by high resolution 2-D electrophoresis

Molecular Reproduction and Development

Volumn 45, Issue 3, 1996, Pages 342-350

  Hendriksen, Peter J M ^a,b   Welch, Glenn R ^c   Grootegoed, J Anton ^b   Van Der Lende, Tette ^a   Johnson, Lawrence A ^c  

^a WAGENINGEN UNIVERSITY   (Netherlands)

^b ERASMUS UNIVERSITY ROTTERDAM   (Netherlands)

^c AGRICULTURAL RESEARCH SERVICE   (United States)

Author keywords

Flow cytometry; Membrane Proteins; Spermatozoa; X Chromosome; Y Chromosome

Indexed keywords

DNA; MEMBRANE PROTEIN;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CHROMOSOME HIGH RESOLUTION BANDING ANALYSIS; DNA CONTENT; ELECTROPHORESIS; MALE; MEMBRANE STRUCTURE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; SPERMATOZOON; SWINE; X CHROMOSOME; Y CHROMOSOME;

ANIMALS; CELL MEMBRANE; CHOLIC ACIDS; DETERGENTS; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; FLOW CYTOMETRY; MALE; MEMBRANE PROTEINS; SPERMATOZOA; SWINE; X CHROMOSOME; Y CHROMOSOME;

ANIMALIA; SUIDAE; SUS SCROFA;

EID: 0029955139     PISSN: 1040452X     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1098-2795(199611)45:3<342::AID-MRD11>3.0.CO;2-0     Document Type: Article

References (50)

1. Ali JI, Eldridge FE, Koo GC, Schanbacher BD (1990): Enrichment of bovine X and Y chromosome-bearing sperm with monoclonal H-Y antibody-fluorescence-activated cell sorter. Arch Andrology 24: 235-245.
2. Amann RP (1989): Treatment of sperm to predetermine sex. Theriogenology 31:49-60.
3. Aranha IP, Martin-DeLeon PA (1992): Evidence for differential maturation of reciprocal sperm segregants in the murine Rb(6.16) translocation heterozygote. Mol Reprod Dev 32:394-398.
4. Aranha IP, Martin-DeLeon PA (1995): Mouse chromosome 6 in Rb translocations: consequences in singly and doubly heterozygous males. Cytogenet Cell Genet 69:253-259.
5. Beernink FJ, Dmowski WP, Ericsson RJ (1993): Sex preselection through albumin separation of sperm. Fertil Steril 59:382-386.
6. Bennett D, Boyse EA (1973): Sex ratio in progeny of mice inseminated with sperm treated with H-Y serum. Nature 246:308-309.
7. Braun RE, Behringer RR, Peschon JJ, Brinster RL, Palmiter RD (1989): Genetically haploid spermatids are phenotypically diploid. Nature 337:373-376.
8. Caldwell KA, Handel MA (1991): Protamine transcript sharing among post-meiotic spermatids. Proc Natl Acad Sci USA 88:2407-2411.
9. Capel B, Swain A, Nicolis S, Hacker A, Walter M, Koopman P, Goodfellow P, Lovell-Badge R (1993): Circular transcripts of the testis-determining gene Sry in adult mouse testis. Cell 73:1019-1030.
10. Chayko CA, Martin-DeLeon PA (1992): The murine Rb(6.16) translocation: alterations in the proportion of alternate sperm segregants effecting fertilization in vitro and in vivo. Hum Genet 90:79-85.
11. Claassens OE, Oosthuizen CJJ, Brusnicky J, Franken DR, Kruger TF (1995): Fluorescent in situ hybridization evaluation of human Y-bearing spermatozoa separated by albumin density gradients. Fertil Steril 63:417-418.
12. Conway SJ, Mahadevaiah SK, Darling SM, Capel B, Rattigan AM, Burgoyne PS (1994): Y353/B: A candidate multiple-copy spermiogenesis gene on the mouse Y chromosome. Mammal Genome 5:203-210.
13. Cran DG, Johnson LA, Miller NGA, Cochrane D, Polge C (1993): Production of bovine calves following separation of X- and Y-chromosome bearing sperm and in vitro fertilisation. Vet Rec 132:40-41.
14. Cran DG, Johnson LA, Polge C (1995): Sex preselection in cattle: a field trial. Vet Rec 136:495-496.
15. Dahl H-HM, Brown RM, Hutchison WM, Maragos C, Brown GK (1990): A testis-specific form of the human pyruvate dehydrogenase E1α subunit is coded for by an intronless gene on chromosome 4. Genomics 8:225-232.
16. De Boer P, Redi CA, Garagna S, Winking H (1990): Protamine amount and cross linking in mouse teratospermatozoa and aneuploid spermatozoa. Mol Reprod Dev 25:297-301.
17. Ericsson RJ, Langevin CN, Nishino M (1973): Isolation of fractions rich in human Y sperm. Nature 246:421-424.
18. Fenner GP, Johnson LA, Hruschka WR, Bolt DJ (1992) Two-dimensional electrophoresis and densitometric analysis of solubilized bovine sperm plasma membrane proteins detected by silver staining and radioiodination. Arch Andrology 29:21-32.
19. Han TL, Flaherty SP, Ford JH, Matthews CD (1993): Detection of X-and Y-bearing human spermatozoa after motile sperm isolation by swim-up. Fertil Steril 60:1046-1051.
20. Harrison RAP, Dott HM, Foster GC (1982): Bovine serum albumin, sperm motility, and the "dilution effect." J Exp Zool 222:81-88.
21. Hendriksen PJM, Tieman M, van der Lende T, Johnson LA (1993): Binding of anti-H-Y monoclonal antibodies to separated X and Y chromosome bearing porcine and bovine sperm. Mol Reprod Dev 35:189-196.
22. Hendriksen PJM, Hoogerbrugge JW, Themmen APN, Koken MHM, Hoeijmakers JHJ, Oostra BA, van der Lende T, Grootegoed JA (1995): Postmeiotic transcription of X and Y chromosomal genes during spermatogenesis in the mouse. Dev Biol 170:730-733.
23. Heukeshoven J, Dernick R (1988): Improved silver staining procedure for fast staining in PhastSystem development unit I. Staining of sodium dodecyl sulfate gels. Electrophoresis 9:28-32.
24. Johnson LA (1991): Sex preselection in swine: altered sex ratios in offspring following surgical insemination of flow sorted X- and Y-bearing sperm. Reprod Dom Anim 26:309-314.
25. Johnson LA (1992): Gender preselection in domestic animals using flow cytometrically sorted sperm. J Anim Sci 70(Suppl 2):8-18.
26. Johnson LA (1994): Isolation of X- and Y-bearing sperm for sex preselection. In HM Charlton (ed): "Oxford Reviews of Reproductive Biology," Volume 16. Oxford, UK: Oxford University Press, pp 303-326.
27. Johnson LA (1995): Sex preselection by flow cytometric separation of X and Y chromosome-bearing sperm based on DNA difference: a review. Reprod Fertil Dev 7:893-903.
28. Johnson LA, Pinkel D (1986): Modification of a laser-based flow cytometer for high-resolution DNA analysis of mammalian spermatozoa. Cytometry 7:268-273.
29. Johnson LA, Flook JP, Look MV (1987): Flow cytometry of X and Y chromosome-bearing sperm for DNA using an improved preparation method and staining with Hoechst 33342. Gamete Res 17:203-212.
30. Johnson LA, Aalbers JG, Grooten HJG (1988): Artificial insemination of swine: fecundity of boar semen stored in Beltsville TS (BTS), Modified Modena (MM), or MR-A and inseminated on one, three and four days after collection. Zuchthygiene 23:49-55.
31. Johnson LA, Flook JP, Hawk HW (1989): Sex preselection in rabbits: Live births from X and Y sperm separated by DNA and cell sorting. Biol Reprod 41:199-203.
32. Johnson LA, Welch GR, Keyvanfar K, Dorfmann A, Fugger EF, Schulman JD (1993): Gender preselection in humans? Flow cytometric separation of X and Y spermatozoa for the prevention of X-linked diseases. Hum Reprod 8:1733-1739.
33. Johnson LA, Welch GR, Garner DL (1994): Improved flow sorting resolution of X- and Y-chromosome bearing viable sperm separation using dual staining and dead cell gating. Cytometry 7(suppl.):83.
34. Jones R (1986): Preparation of a plasma membrane-rich fraction from rat spermatozoa. J Reprod Fert 77:435-449.
35. Klint M, Sege K, Curman B, Ploën L, Peterson PA (1985): Solubilization and enrichment of boar sperm membrane proteins. Gamete Res 11:335-348.
36. Levinson G, Keyvanfar K, Wu JC, Fugger EF, Fields RA, Harton GL, Palmer FT, Sisson ME, Starr KM, Dennison-Lagos L, Calvo L, Sherins RJ, Bick D, Schulman JD, Black SH (1995): DNA-based X-enriched sperm separation as an adjunct to preimplantation genetic testing for the prevention of X-linked disease. Hum Reprod 10: 979-982.
37. McCarrey JR, Dilworth DD, Sharp RM (1992): Semiquantitative analysis of X-linked gene expression during spermatogenesis in the mouse: ethidium bromide staining of RT-PCR products. Genet Anal Tech Appl 9:117-123.
38. McNutt TL, Rogowski L, Vasilatos-Younken R, Kilian G (1992): Adsorption of oviductal fluid proteins by the bovine sperm membrane during in vitro capacitation. Mol Reprod Dev 33:313-323.
39. McNutt TL, Johnson LA (1996): Electrophoretic gel analysis of Hoechst 33342 stained and flow cytometrically sorted bovine sperm membrane proteins. Reprod Dom Anim (in press).
40. Moriwaki K, Suh D-S, Styrna J (1988): Genetic factors governing sperm morphology of the mouse. Mouse News Lett 82:138.
41. Nagamine CM, Chan K, Hake LE, Lau Y-FC (1990): The two candidate testis-determining Y genes (Zfy-1 and Zfy-2) are differentially expressed in fetal and adult mouse tissues. Genes Dev 4:63-74.
42. Olds-Clarke P (1988): Genetic analysis of sperm function in fertilization. Gamete Res 20:241-264.
43. Roberts AM (1972): Gravitational separation of X- and Y-spermatozoa. Nature (London) 238:223-225.
44. Shannon M, Handel MA (1993): Expression of the Hprt gene during spermatogenesis: Implications for sex-chromosome inactivation. Biol Reprod 49:770-778.
45. Sonta S, Yamada M, Tsukasaki M (1991): Failure of chromosomally abnormal sperm to participate in fertilization in the Chinese hamster. Cytogenet Cell Genet 57:200-203.
46. Ueda K, Yanagimachi R (1987): Sperm chromosome analysis as a new system to test human X- and Y-sperm separation. Gamete Res 17: 221-228.
47. Van Kooij RJ, van Oost BA (1992): Determination of sex ratio of spermatozoa with a deoxyribonucleic acid-probe and quinacrine staining: a comparison. Fertil Steril 58:384-386.
48. Wang H-X, Flaherty SP, Swann NJ, Matthews CD (1994): Assessment of the separation of X- and Y-bearing sperm on albumin gradients using double-label fluorescence in situ hybridization. Fertil Steril 61:720-726.
49. Welch GR, Waldbieser GC, Wall RJ, Johnson LA (1995): Flow cytometric sperm sorting and PCR to confirm separation of X- and Y-chromosome bearing bovine sperm. Anim Biotechnol 6:131-139.
50. Windsor DP, Evans G, White IG (1993): Sex predetermination by separation of X and Y chromosome-bearing sperm: A review. Reprod Fertil Dev 5:155-171.