Microheterogeneity of human filaggrin: Analysis of a complex peptide mixture using mass spectrometry

Protein Science

Volumn 5, Issue 6, 1996, Pages 1157-1164

  Thulin, Craig D ^a,b   Taylor, J Alex ^a   Walsh, Kenneth A ^a  

^a UNIVERSITY OF WASHINGTON SCHOOL OF MEDICINE   (United States)

^b OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

Author keywords

filaggrin; mass spectrometry; microheterogeneity; peptide mapping; profilaggrin

Indexed keywords

AMINO ACID; COMPLEMENTARY DNA; FILAGGRIN; PEPTIDE; PROFILAGGRIN;

AMINO ACID SEQUENCE; ARTICLE; GENE SEQUENCE; HUMAN; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PEPTIDE MAPPING; PRIORITY JOURNAL; PROTEIN DEGRADATION;

EID: 0029953828     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1002/pro.5560050618     Document Type: Article

References (13)

1. Biemann K. 1990. Sequencing of peptides by tandem mass spectrometry and high-energy collision-induced dissociation. Methods Enzymol 193:455-480.
2. Dale BA, Presland RB, Fleckman P, Kam E, Resing KA. 1993. Phenotypic expression and processing of filaggrin in epidermal differentiation. In: Darmon M, Blumenberg M, eds. Molecular biology of the skin; the keratinocyte. San Diego: Academic Press. pp 79-106.
3. Downard KM, Biemann K. 1995 Charging behavior of highly basic peptides during electrospray ionization. A predilection for protons. Intl J Mass Spectr and Ion Processes 148:191-202.
4. Gan SQ, McBride OW, Idler WW, Markova N, Steinert PM. 1990. Organization, structure, and polymorphisms of the human profilaggrin gene [erratum published in 1991 Biochemistry.30:5814]. Biochemistry 29(40): 9432-9440.
5. Hillenkamp F, Karas M. 1990. Mass spectrometry of peptides and proteins by matrix-assisted ultraviolet laser desorption/ionization. Meth Enzymol 193:280-295.
6. McKinley-Grant LJ, Idler WW, Bernstein IA, Parry DA, Cannizzaro L, Croce CM, Huebner K, Lessin SR, Steinert PM. 1989. Characterization of a cDNA clone encoding human filaggrin and localization of the gene to chromosome region 1q21. Proc Natl Acad Sci USA 86(13):4848-4852.
7. Presland RB, Haydock PV, Fleckman P, Nirunsuksiri W, Dale BA. 1992. Characterization of the human epidermal profilaggrin gene. Genomic organization and identification of an S-100-like calcium binding domain at the amino terminus. J Biol Chem 267(33):23772-23781.
8. Resing KA, Dale BA, Walsh KA. 1985. Multiple copies of phosphorylated filaggrin in epidermal profilaggrin demonstrated by analysis of tryptic peptides Biochemistry 24(15):4167-4175.
9. Resing KA, Johnson RS, Walsh KA. 1993. Characterization of protease processing sites during conversion of rat profilaggrin to filaggrin. Biochemistry 32(38):10036-10045.
10. Rothnagel JA, Steinert PM. 1990. The structure of the gene for mouse filaggrin and a comparison of the repeating units. J Biol Chem 265(4): 1862-1865.
11. Taylor JA, Walsh KA, Johnson RS. 1996. Sherpa: A Macintosh-based expert system for the interpretation of ESI LC/MS and MS/MS of protein digests. Rapid Commun Mass Spectrom. Forthcoming.
12. Thulin CD. 1995. Posttranslational processing and human profilaggrin [dissertation]. Seattle, Washington: University of Washington.
13. Thulin CD, Walsh KA. 1995 Identification of the amino terminus of human filaggrin using differential LC/MS techniques; implications for profilaggrin processing. Biochemistry 34(27):8687-8692.