Transient transfection of primary cultured hepatocytes using CaPO4/DNA precipitation

BioTechniques

Volumn 20, Issue 5, 1996, Pages 826-832

  Gaunitz, Frank ^a   Papke, Monika ^a   Gebhardt, Rolf ^a  

^a UNIVERSITY OF TÜBINGEN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

LUCIFERASE;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; DNA TRANSFECTION; LABORATORY TEST; LIVER CELL; MALE; NONHUMAN; PRECIPITATION; RAT; REPORTER GENE;

ANIMALIA;

EID: 0029953690     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96205st01     Document Type: Article

References (10)

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3. Chen, C. and H. Okayama. 1987. High-efficiency transformation of mammalian cells by plasmid DNA. Mol. Cell. Biol. 7:2745-2752.
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8. Nordeen, S.K. 1988. Luciferase reporter gene vectors for analysis of promoters and enhancers. BioTechniques 6:454-458.
9. Pasco, D.S. and J.B. Fagan. 1989 Efficient DNA-mediated gene transfer into primary cultures of adult rat hepatocytes. DNA 8:535-541.
10. Ponder, K.P., R.P. Dunbar, D.R. Wilson, G.J. Darlington and S.L.C. Woo. 1991. Evaluation of relative promoter strength in primary hepatocytes using optimized lipofection. Hum. Gene Ther. 2:41-52.