Age-related changes in neutral lipid content of Paramecium primaurelia as revealed by nile red

Journal of Lipid Research

Volumn 37, Issue 6, 1996, Pages 1207-1212

  Ramoino, Paola ^a   Margallo, Edoardo ^b   Nicolò, Guido ^b  

^a UNIVERSITY OF GENOA   (Italy)

^b UNIVERSITY OF GENOA   (Italy)

Author keywords

ciliated protozoa; clonal life; culture growth; fluorescence microscopy; food ingestion capacity; lipid droplets

Indexed keywords

AGING; ARTICLE; CELL CULTURE; FLUORESCENCE MICROSCOPY; FOOD INTAKE; LIPID ANALYSIS; LIPID COMPOSITION; PARAMECIUM; PRIORITY JOURNAL;

ANIMALS; FLUORESCENT DYES; LIPID METABOLISM; MICROSCOPY, FLUORESCENCE; OXAZINES; PARAMECIUM; SPECTROMETRY, FLUORESCENCE; TIME FACTORS; VACUOLES;

EID: 0029952345     PISSN: 00222275     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (26)

1. Greenspan, P., E. P. Mayer, and S. D. Fowler. 1985. Nile red: a selective fluorescent stain for intracellular lipid droplets. J. Cell Biol. 100: 965-973.
2. Goldstein, J. L., and M. S. Brown. 1977. The low-density lipoprotein pathway and its relation to atherosclerosis. Annu. Rev. Biochem. 46: 897-930.
3. Brown, M. S., and J. L. Goldstein. 1983. Lipoprotein metabolism in the macrophage: implications for cholesterol deposition in atherosclerosis. Annu. Rev. Biochem. 52: 223-261.
4. Greenspan, P., and S. D. Fowler. 1985. Spectrofluorometric studies of the lipid probe, nile red. J. Lipid Res. 26: 781-789.
5. Fowler, S. D., and P. Greenspan. 1985. Application of nile red, a fluorescent hydrophobic probe, for the detection of neutral lipid deposits in tissue sections: comparison with Oil red O. J. Histochem. Cytochem. 33: 833-836.
6. Ducibella, T., and B. E. Batten. 1987. Lipid distributions in mouse eggs and preimplantation embryos as revealed by the vital fluorescent dye nile red. Anat. Record. 218: A36-37.
7. Brown, W., J. Warfel, and P. Greenspan. 1988. Use of nile red in the detection of cholesteryl ester accumulation in acid-lipase-deficient fibroblasts. Arch. Pathol. Lab. Med. 112: 295-297.
8. Santilli, I., A. Prelle, L. Geremia, G. Scarlato, and G. Meola. 1990. Nile red simultaneous staining of intracellular lipids and membrane network in human muscle cultures. Bas. Appl. Histochem. 33: 49-52.
9. Cole, T. A., A. K. Fok, M. S. Ueno, and R. D. Allen. 1990. Use of nile red as a rapid measure of lipid content in ciliates. Eur. J. Protistol. 25: 361-368.
10. Fok, A. K., R. D. Allen, and E. S. Kaneshiro. 1981. Axenic Paramecium caudatum. III. Biochemical and physiological changes with culture age. Eur. J. Cell Biol. 25: 193-201.
11. Sonneborn, M. T. 1970. Methods in Paramecium research. In Methods in Cell Physiology. D. M. Prescott, editor. Academic Press, New York. 4: 241-339.
12. Dippell, R. V. 1955. A temporary stain for Paramecium and other ciliate protozoa. Stain Technol. 30: 69-71.
13. Kaneshiro, E. S. 1987. Lipids of Paramecium. J. Lipid Res. 28: 1241-1258.
14. Kaneshiro, E. S. 1990. Lipids of ciliary and flagellar membranes. In Ciliary and Flagellar Membranes. R. A. Bloodgood, editor. Plenum Publishing Corp., New York. 241-265.
15. Kaneshiro, E. S., L. S. Beischel, S. J. Merkel, and D. E. Rhoads. 1979. The fatty acid composition of Paramecium aurelia cells and cilia: changes with culture age. J. Protozool. 26: 147-158.
16. Kaneshiro, E. S., K. B. Meyer, and M. L. Reese. 1983. The neutral lipids of Paremecium tetraurelia: changes with culture age and the detection of steryl esters in ciliary membranes. J. Protozool. 30: 392-396.
17. Rhoads, D. E., and E. S. Kaneshiro. 1979. Characterization of phospholipids from Paramecium tetraurelia cells and cilia. J. Protozool. 26: 329-338.
18. Hill, D. L. 1972. The Biochemistry and Physiology of Tetrahymena. Academic Press, New York. 46-55.
19. Holz, G. G., Jr., and R. L. Conner. 1973. The composition, metabolism, and roles of lipids in Tetrahymena. In Biology of Tetrahymena. A. M. Elliott, editor. Dowden Hutchunson & Ross, Stroudsburg. 99-122.
20. Soldo, A. T., G. A. Godoy, and W. J. van Wagtendonk. 1966. Growth of particle-bearing and particle-free Paramecium aurelia in axenic culture. J. Protozool. 13: 492-497.
21. Sonneborn, T. M. 1954. The relation of autogamy to senescence and rejuvenescence in Paramecium aurelia. J. Protozool. 1: 38-53.
22. Fok, A. K., and R. D. Allen. 1979. Axenic Paramecium caudatum. Mass culture and structure. J. Protozool. 26: 463-470.
23. Mast, S. O. 1947. The food-vacuoles in Paramecium. Biol. Bull. 92: 31-72.
24. Reuter, S. F., P. J. Soldano, and E. S. Kaneshiro. 1989. Uptake and utilization of lipids by Paramecium tetraurelia. Eur. J. Cell Biol. 49: 61-65.
25. Reuter, S. F., R. L. Prairie, and E. S. Kaneshiro. 1993. The kinetics of fatty acid uptake by Paramecium tetraurelia. J. Eukaryot. Microbiol. 40: 370-376.
26. Ramoino, P., F. Beltrame, and M. Fato. 1995. Image analysis of lysosomal activity during the early clonal life of Paramecium primaurelia. FEMS Microbiol. Lett. 125: 57-62.