A rapid microcentrifuge procedure for purification of Cryptosporidium sporozoites

Journal of Microbiological Methods

Volumn 25, Issue 1, 1996, Pages 87-89

  Nesterenko, M V ^a   Upton, S J ^a  

^a KANSAS STATE UNIVERSITY   (United States)

Author keywords

centrifugation; Cryptosporidium parvum; Percoll ; purification

Indexed keywords

ARTICLE; CENTRIFUGATION; CONTROLLED STUDY; CRYPTOSPORIDIUM PARVUM; NONHUMAN; PRIORITY JOURNAL; SPOROZOITE;

EID: 0029951876     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/0167-7012(95)00083-6     Document Type: Article

References (9)

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2. Current, W.L. (1990). Technqiues and laboratory maintenance of Cryptosporidium. In: Cryptosporidiosis of Man and Animals (Dubey, J.P., Speer, C.A. and Fayer, R., eds.), pp. 31-49, CRC Press, Boca Raton, Florida.
3. Taghi-Kilani, R. and Sekla, L. (1987) Purification of Cryptosporidium oocysts and sporozoites by cesium chloride and percoll gradients. Am. J. Trop. Med. Hyg. 36, 505-508.
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6. Fukata, T., Sasai, K., Arakawa, A. and McDougald, L. R. (1992) Penetration of Eimeria tenella sporozoites under different oxygen concentrations in vitro. J. Parasitol. 78, 537-538.
7. Upton, S.J. and Tilley, M. (1995) Effects of reduced oxygen atmosphere on motility, penetration of host cells, and intracellular survival of Eimeria nieschulzi sporozoites in vitro. J. Helminthol. Soc. Wash. 62, 223-225.
8. Wrede, D., Salisch, H. and Siegmann, O. (1993) Oxygen concentration and asexual development of Eimeria tenella in cell cultures. J. Vet. Med. B 40, 391-396.
9. Upton, S.J., Tilley, M., Nesterenko, M.V. and Brillhart, D.B. (1994) A simple and reliable method of producing in vitro infections of Cryptosporidium parvum (Apicomplexa). FEMS Microbiol. Lett. 118, 45-50.