Immunopotency of a viral peptide assembled on the carbohydrate moieties of self immunoglobulins

Nature Biotechnology

Volumn 14, Issue 6, 1996, Pages 722-725

  Brumeanu, Teodor D ^a   Casares, Sofia ^a   Harris, Paul E ^b   Dehazya, Philip ^a   Wolf, Imre ^a   Von Boehmer, Harold ^c   Bona, Constantin A ^a  

^a MOUNT SINAI SCHOOL OF MEDICINE   (United States)

^b Department of Pathology   (United States)

^c BASEL INSTITUTE FOR IMMUNOLOGY   (Switzerland)

Author keywords

Carbohydrate; Immunoglobulin; Immunopotency; Viral epitope

Indexed keywords

AUTOANTIBODY; CARBOHYDRATE; CD4 ANTIGEN; CD8 ANTIGEN; CHIMERIC ANTIBODY; CYTOKINE; EPITOPE; IMMUNOGLOBULIN; MAJOR HISTOCOMPATIBILITY ANTIGEN; SYNTHETIC PEPTIDE; VIRUS ANTIGEN; VIRUS HEMAGGLUTININ;

ANIMAL CELL; ANTIGEN PRESENTING CELL; ANTIGEN RECOGNITION; ARTICLE; AUTOIMMUNITY; CELL PROLIFERATION; CONTROLLED STUDY; GENETIC ENGINEERING; HYBRIDOMA; IMMUNOGENICITY; IMMUNOGLOBULIN GENE; IMMUNOGLOBULIN STRUCTURE; IMMUNOMODULATION; INFECTION; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; T LYMPHOCYTE ACTIVATION; T LYMPHOCYTE SUBPOPULATION;

ANIMALS; CARBOHYDRATES; CD4-POSITIVE T-LYMPHOCYTES; CD8-POSITIVE T-LYMPHOCYTES; CELL LINE; IMMUNOGLOBULINS; LYMPHOCYTE ACTIVATION; MICE; MICE, INBRED BALB C; MICE, TRANSGENIC; PEPTIDES; VIRAL PROTEINS;

EID: 0029951853     PISSN: 10870156     EISSN: 15461696     Source Type: Journal    
DOI: 10.1038/nbt0696-722     Document Type: Article

References (16)

1. Leclerc, C. (ed). 1994. Immunogenicity of foreign epitopes expressed in chimeric molocules. Int. Rev. Immun. 11:103-167.
2. Billeta, R., Hollingdale, M.R., and Zanetti, M. 1991. Immunogenicity of an engineered internal image antibody. Proc. Natl. Acad. Sci. USA 88:4713-4717.
3. Zaghouani, H., Steinman, R.M., Novacs, R., Shah, H., Gerhard, W.V., and Bona, C.A. 1993. Presentation of a viral peptide T-cell epitope expressed in the CDR3 region of a self immunoglobulin molecule. Science 259:224-227.
4. Zaghouani, H., Anderson, S.A., Kennedy, R.C., Mayer, L., and Bona, C. 1995. Induction of antibodies to the human immunodeficiency virus type I by immunization of baboons with immunoglobulin molecules carrying the principal neutralizing determinant of the envelope protein. Proc. Natl. Acad. Sci. USA 92:631-635.
5. Zaghouani, H., Krystal, M., Kuzu, H., Morah, T., and Bona, C. 1992. Cells expressing an heavy chain immunoglobulin gene carrying a viral T-cell epitope are lysed by specific cytolltic Τ cells. J. Immunol. 148:3604-3609.
6. Lee, G. 1993. Strong inhibition of fibrinogen binding to platelet receptor αllβ3 by RAD sequences installed into a presentation scaffold. Protein Engin. 6:745-754.
7. Brumeanu, T-D., Dehazya, P., Wolf, I., and Bona, C.A. 1995. Enzymatically mediated, glycosidic conjugation of immunoglobulins with viral epitopes. J. Immunol. Methods 183/2:185-197.
8. Haberman, A.M., Moller, A.M., McCreedy, CD., and Gerhard, W.V. 1990. A large degree of functional diversity exists among helper Τ cells specific for the same antigenic site of influenza hemagglutinin. J. Immunol. 145:3087-3094.
9. Spencer, J. and Cubo, R.T. 1989. Mixed isotype class II antigen expression. A novel class II molecule is expressed on murine B-cell lymphoma. J. Exp. Med. 169:625-640.
10. Caton, A.J., Brownlee, G.C., Yewdel, J.W., and Gerhard, W.V. 1982. The antigenic structure of the Influenza virus PR8/A/8/34 hemagglutinin. Cell 31:417-427.
11. Weber, S., Trannecker, A., Oliveri, F., Gerhard, W.V., and Karjalainen, K. 1992. Specific low-affinity recognition of MHC complex plus peptide by soluble T-cell receptor. Nature 356:793-796.
12. Kirberg, J., Baron, A., Jacob, S., Rolink, A., Karjalainen, K., and von Boehmer, H. 1994. Thymic selection of CD8 single positive cells with a major histocompatibility complex-restricted receptor. J. Exp. Med. 180:25-34.
13. Brumeanu, T.-D., Swiggard, W.J., Steinman, R.M., Bona, CA., and Zaghouani, H. 1993. Efficient loading of identical viral peptide onto class II molecules by antigenized immunoglobulin and Influenza virus. J. Exp. Med. 178:1795-1799.
14. Brumeanu, T.-D., Kohanski, R., Bona, C.A., and Zaghouani, H. 1993. A sensitive method to detect defined peptide among those eluted from class II molecules. J. Immunol. Methods 160:65-71.
15. Brumeanu, T.-D., Dehazya, P., Bot, S., Casares, S., Wolf, I., and Bona, C. 1996. MHC class II presentation of a viral epitope assembled on the carbohydrate moieties of self Immunoglobulins does not require antigen processing. Submitted.
16. Mossman, T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 65:55-63.