Differentiating allele combinations of a transgene using multiple primer PCR

BioTechniques

Volumn 20, Issue 6, 1996, Pages 956-960

  Pekhletski, Roman ^a   Hampson, David R ^a  

^a UNIVERSITY OF TORONTO   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT; DNA POLYMERASE;

ALLELE; ANIMAL CELL; ARTICLE; CELL DIFFERENTIATION; DNA TEMPLATE; GENE AMPLIFICATION; GENE TARGETING; GENOTYPE; MOUSE; NONHUMAN; POLYMERASE CHAIN REACTION; TRANSGENE;

ALLELES; ANIMALS; BASE SEQUENCE; BLOTTING, SOUTHERN; DNA PRIMERS; MICE; MICE, TRANSGENIC; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; RECEPTORS, METABOTROPIC GLUTAMATE; STEM CELLS; TRANSGENES;

ANIMALIA;

EID: 0029949372     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96206bm02     Document Type: Article

References (5)

1. Chatelain, G., G. Brun and D. Michel. 1995. Screening of homozygous transgenic mice by comparative PCR. BioTechniques 18:958-962.
2. Padegimas, L., O.A. Shul'ga and K.G. Skriabin. 1993. Testing of transgenic plants using the polymerase chain reaction. Mol. Biol. 27:947-951.
3. Peng, H.Z., RG. Isaacson, T.C. Diss and L.X. Pan. 1994. Multiple PCR analyses on trace amounts of DNA extracted from fresh and paraffin wax embedded tissues after random hexamer primer PCR amplification. J. Clin. Pathol. 47:605-608.
4. Siebert, P.D. and J.W. Larrick. 1993. PCR MIMICS: competitive DNA fragments for use as internal standards in quantitative PCR. BioTechniques 14:244-249.
5. Ward, M., W. Abramow-Newerly, B. Clapoff and J.C. Roder. 1992. Analysis of transgenic animal pedigrees using the junction-polymerase chain reaction. PCR Methods Appl. 1:208-209.