Iron-regulated transcription of the pvdA gene in Pseudomonas aeruginosa: Effect of fur and PvdS on promoter activity

Journal of Bacteriology

Volumn 178, Issue 8, 1996, Pages 2299-2313

  Leoni, Livia ^a   Ciervo, Alessandra ^a,b   Orsi, Nicola ^a   Visca, Paolo ^a  

^a SAPIENZA UNIVERSITY OF ROME   (Italy)

^b ISTITUTO SUPERIORE DI SANITÀ   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

PSEUDOMONAS; PSEUDOMONAS AERUGINOSA;

ARTICLE; GENE EXPRESSION REGULATION; GENE LOCATION; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PSEUDOMONAS AERUGINOSA; REPRESSOR GENE; TRANSCRIPTION REGULATION;

BACTERIAL PROTEINS; BASE SEQUENCE; GENE EXPRESSION REGULATION, BACTERIAL; GENES, BACTERIAL; IRON; MIXED FUNCTION OXYGENASES; MOLECULAR SEQUENCE DATA; PROMOTER REGIONS (GENETICS); PSEUDOMONAS AERUGINOSA; REPRESSOR PROTEINS; SEQUENCE ANALYSIS, DNA; SIGMA FACTOR; TRANSCRIPTION, GENETIC;

EID: 0029949202     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.178.8.2299-2313.1996     Document Type: Article

References (68)

1. Ankenbauer, R., L. F. Hanne, and C. D. Cox. 1986. Mapping of mutations in Pseudomonas aeruginosa defective in pyoverdin production. J. Bacteriol. 167: 7-11.
2. Ankenbauer, R., S., and H. N. Quan. 1994. FptA, the Fe(III)-pyochelin receptor of Pseudomonas aeruginosa: a phenolate siderophore receptor homologus to hydroxamate siderophore receptors. J. Bacteriol. 176:307-319.
3. Ankenbauer, R., S. Sriyosachati, and C. D. Cox. 1985. Effects of siderophores on the growth of Pseudomonas aeruginosa in human serum and transferrin. Infect. Immun. 49:132-140.
4. Bagdasarian, M. M., E. Amann, R. Lurz, B. Ruckert, and M. Bagdasarian. 1983. Activity of the hybrid trp-lac (tac) promoter of Escherichia coli in Pseudomonas putida. Construction of broad-host-range, controlled-expression vectors. Gene 26:273-282.
5. Barton, H. A., Z. Johnson, A. I. Vasil, and M. L. Vasil. Ferric uptake regulator mutants of Pseudomonas aeruginosa with distinct alterations in the iron dependent repression of exotoxin A and siderophores in aerobic and microaerobic environments. Mol. Microbiol. Submitted for publication.
6. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
7. Briskot, G., K. Taraz, and H. Budzikiewicz. 1989. Pyoverdin-type siderophores from Pseudomonas aeruginosa. Liebigs Ann. Chem. 37:375-384.
8. Brumlik, M. J., and D. G. Storey. 1992. Zinc and iron regulate translation of the gene encoding Pseudomonas aeruginosa elastase. Mol. Microbiol. 6: 337-344.
9. Bujard, H., R. Gentz, M. Lanzer, D. Staeber, M. Mueller, L. Ibrahimi, M.-T. Haeuptle, and B. Dobbersetin. 1987. A T5 promoter-based transcription-translation system for the analysis of proteins in vitro and in vivo. Methods Enzymol. 115:416-433.
10. Bullen, J. J., H. J. Rogers, and E. Griffiths. 1978. Role of iron in bacterial infection. Curr. Top. Microbiol. Immunol. 80:1-35.
11. Casabadan, M. J., and S. N. Cohen. 1980. Analysis of gene control signals by DNA fusion and cloning in Escherichia coli J. Mol. Biol. 138:179-207.
12. Certa, U., W. Bannwarth, D. Stueber, R. Gentz, M. Lanzer, S. Le Grice, F. Giullot, I. Wendler, G. Hunsmann, H. Bujard, and J. Mous. 1986. Subregions of a conserved part of the HIV gp41 transmembrane protein are differentially recognized by antibodies of infected individuals. EMBO J. 5: 3051-3056.
13. Chang, A. C. Y., and S. N. Cohen. 1978. Phenotypic expression in Escherichia coli of a DNA sequence coding for mouse dihydrofolate reductase. J. Bacteriol. 134:1141-1156.
14. Cox, C. D. 1986. Relationship between oxygen and siderophore synthesis in Pseudomonas aeruginosa. Curr. Microbiol 14:19-23.
15. Cunliffe, H. E., T. R. Merriman, and I. L. Lamont. 1995. Cloning and characterization of pvdS, a gene required for pyoverdine synthesis in Pseudomonas aenigmosa: PvdS is probably an alternative sigma factor. J. Bacteriol. 177:2744-2750.
16. Dean, C. R., and K. Poole. 1993. Cloning and characterization of the ferric enterobactin receptor gene (pfeA) of Pseudomonas aeniginosa. J. Bacteriol. 175:317-324.
17. de Lorenzo, V., F. Giovannini, M. Herrero, and J. B. Neilands. 1988. Metal ion regulation of gene expression. Fur repressor-operator interaction at the promoter region of the aerobactin system of pCoIV-K30. J Mol. Biol 203: 875-884.
18. Deretic, V., W. M. Konyecsni, C. D. Mohr, D. W. Marin, and N. S. Hibler. 1989. Common determinators of promoter control in Pseudomonas and other bacteria. Bio/Technology 7:1249-1254.
19. Espelund, M., R. A. Prentice Stacy, and K. S. Jakobson. A simple method for generating single-stranded DNA probes labeled to high activities. Nucleic Acids Res 18:6157-6158.
20. Figurski, D. H., and D. R. Helinski. 1979. Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans. Proc. Natl. Acad. Sci. USA 76:1648-1652.
21. Frank, D. W., D. G. Storey, M. S. Hindahl, and B. Iglewski. 1989. Differential regulation by iron of regA and toxA transcript accumulation in Pseudomonas aeniginosa. J. Bacteriol. 171:5304-5313.
22. Gammie, A, E., and J. H. Crosa. 1991. Co-operative autoregulation of a replication protein gene. Mol. Microbiol. 5:3015-3023.
23. Gamper, M., B. Ganter, M. R. Polito, and D. Haas. 1992. RNA processing modulates the expression of the arcDABC operon in Pseudomonas aeruginosa. J. Mol. Biol. 226:943-957.
24. Gillam, A. H., A. G. Lewis, and R. J. Andersen. 1981. Quantitative determination of hydroxamic acids. Anal. Chem. 53:841-844
25. Haas, B., J. Kraut, J. Marks, S. Cassin Zanker, and D. Castagnetti. 1991. Siderophore presence in sputa of cystic fibrosis patients. Infect. Immun. 59: 3997-4000.
26. Heinrichs, D. E., and K. Poole. 1993. Cloning and sequence analysis of a gene (pchR) encoding an AraC family activator of pyochelm and ferripyochelin receptor synthesis in Pseudomonas aeruginosa. J. Bacteriol. 175:588-5889.
27. Holder, I. A., Z. Akchich, and J.-M. Meyer. 1994. Pyoverdin iron binding and virulence of Pseudomonas ueruginosa, abstr. B-49, p. 37. In Abstracts of the 94th General Meeting of the American Society for Microbiology 1994 American Society for Microbiology, Washington, D.C.
28. Hunt, M. C., G. S. Pettis, and M. A. McIntosh. 1994. Promoter and operator determinants for Fur-mediated iron regulation in the bidirectional fepA-fes control region of the Escherichia coli enterobactin gene system. J. Bacteriol 176:3944-3955.
29. Jin, S., K. Ishimoto, and S. Lory. 1994. Nucleotide sequence of the rpo.V gene and characterization of two downstream open reading frames in Pseudomonas aeruginosa. J. Bacteriol. 176:1316-1322.
30. Johnson, B. H., and M. H. Hecht. 1994. Recombinant proteins can be isolated from E coli cells by repeated cycles of freezing and thawing. Biol Technology 12:1357-1360.
31. Koster, M., W. Klompenburg, W. Bittner, J. Leong, and P. Weisbeek. 1994. Role of the outer membrane ferric siderophore receptor PupB in signal transduction across the bacterial cell envelope. EMBO J. 13:2805-2813.
32. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680-685.
33. Litwin, C. M., and S. B. Calderwood. 1993. Role of iron in regulation of virulence genes. Clin. Microbiol. Rev. 6:137-149.
34. Lonetto, M. A., K. L. Brown, K. E. Rudd, and M. J. Buttner. 1994. Analysis of Streptomyces coelicolor sigE gene reveals the existence of a subfamily of eubacterial RNA polymerase a factors involved in the regulation of extra-cytoplasmic functions. Proc. Natl. Acad. Sci. USA 91:7573-7577.
35. Merriman, T. R., M. E. Merriman, and I. L. Lamont. 1995. Nucleotide sequence of pvdD, a pyoverdine biosynthetic gene from Pseudomonas aeruginosa: pvdD has similarity to peptide synthases. J. Bacteriol. 177:252-258.
36. Miller, J. H. 1972. Experiments in molecular genetics, p. 252-255 Cold Spring Harbor Laboratory, Cold Spring Harbor, N Y.
37. Miyazaki, H., H. Kato, T. Nakazawa, and M. Tsuda. 1995. A positive regulatory gene, pvdS, for expression of pyoverdin biosynthetic genes in Pseudomonas aeruginosa PAO. Mol. Gen. Genet 248:17-24.
38. Neilands, J. B. 1981. Iron absorption and transport in microorganisms Annu Rev. Nutr. 1:27-46.
39. Ochsner, U. A., A. I. Vasil, and M. L. Vasil. 1995. Role of ferric uptake regulator of Pseudomonas aeruginosa in the regulation of siderophore and exotoxin A expression, purification and activity on iron-regulated promoter J. Bacteriol. 177:7194-7201.
40. O'Sullivan, D. J., D. N. Dowling, V. de Lorenzo, and F. O'Gara. 1994 Eschenchia coll ferric uptake regulator (Fur) can mediate regulation of a pseudomonad iron-regulated promoter. FEMS Microbiol. Lett 117:327-332.
41. O'Sullivan, D. J., and F. O'Gara. 1991. Regulation of iron assimilation: nucleotide sequence analysis of an iron-regulated promoter from a fluorescent pseudomonad. Mol Gen. Genet. 228:1-8.
42. Peterson, M. B., E. P. Economou, S. A. Slaugenhaupt, A. Chakravarti, and S. E. Antonarakis. 1990. Linkage analysis of the human HGM 14 gene on chromosome 21 using GT nucleotide repeat as polymorphic marker. Genomics 7:136-138.
43. Poole, K., D. E. Heinrichs, and S. Neshat. 1993. Cloning and sequence analysis of an EnvCD homologue in Pseudomonas aeruginosa: regulation by iron and possible involvement in the secretion of the siderophore pyoverdine Mol. Microbiol. 10:529-544.
44. Poole, K., S. Neshat, K. Krebes, and D. E. Heinrichs. 1993. Cloning and nucleotide sequence of the ferripyoverdine receptor gene fpvA of Pseudomonas aeruginosa. J. Bacteriol. 175:4597-4604.
45. Prince, R. W., C. D. Cox, and M. L. Vasil. 1993. Coordinate regulation of siderophore and exotoxin A production: molecular cloning and sequencing of the Pseudomonas aeruguiosa fur gene. J. Bacteriol. 175:2589-2598.
46. Prince, R. W., D. G. Storey, A. I. Vasil, and M. L. Vasil. 1991. Regulation of toxA and regA by the Escherichia coli fur gene and identification of a Fur homologue in Pseudomonas aeruginosa PA103 and PAO1. Mol. Microbiol. 5: 2823-2831
47. Kombel, I. T., B. J. McMorran, and I. L. Lamont. 1994 Identification of a DNA sequence motif required for expression of iron-regulated genes in pseudomonads. Mol Gen. Genet. 246:519-528.
48. Ronald, S., M. A. Farinha, B. J. Allen, and A. M. Kropinski. 1992 Cloning and physical mapping of transcriptional regulatory (sigma) factors from Pseudomonas aeruginosa, p 249-257. In E. Galli, S. Silver, and B. Witholt (ed), Pseudomonas: molecular biology and biotechnology. American Society for Microbiology, Washington, D.C.
49. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989 Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y
50. E) which controls mucoidy in cystic fibrosis isolates of Pseudomonas aeruginosa J Bacteriol. 177:5670-5679.
51. Schweizer, H. P. 1991. Escherichia-Pseudomotws shuttle vectors derived from pUC18/19. Gene 97:109-112.
52. Sexton, R., P. R. Gill, Jr., M. J. Callanan, D. J. O'Sullivan, D. N. Dowling, and F. O'Gara. 1995 Iron-responsive gene expression in Pseudomonas fluorescens M114: cloning and characterization of a transcription-activating factor, PbrA. Mol. Microbiol. 15:297-306.
53. Spaink, H. P., R. J. H. Okker, C. A. Wijffelman, E. Pees, and B. J. J. Lugtenberg. 1987. Promoters in the nodulation region of the Rhizobtum leguminosarum Sym plasmid pRL1J1 Plant Mol Biol. 9:27-39.
54. Sriyosachati, S., and C. D. Cox. 1986. Siderophore-mediated iron acquisition from transferrin by Pseudomonas aeruginosa. Infect. Immun. 52:885-891.
55. Stojilkovic, I., A. J. Bäumler, and K. Hantke. 1994. Fur regulon in Gram-negative bacteria. Identification and characterization of new iron-regulated Escherichia coli genes by a Fur titration assay. J. Mol Biol. 236:531-544.
56. Tsaur, M.-L., and R. C. Clowes. 1989. Localization of the control region for expression of exotoxin A in Pseudomonas aeruginosa. J. Bacteriol. 171:2599-2604.
57. Tsuda, M., H. Miyazaki, and T. Nakazawa. 1995. Genetic and physical mapping of genes involved in pyoverdin production in Pseudomonas aeruginosa PAO. J. Bacteriol. 177:423-431.
58. Venturi, V., C. Ottevanger, J. Leong, and P. J. Weisbeek. 1993 Identification and characterization of a siderophore regulatory gene (pfrA) of Pseudomonas putida WCS358: homology to the alginate regulatory gene algQ of Pseudomonas aeruginosa. Mol Microbiol. 10:63-73.
59. Venturi, V., P. Weisbeek, and M. Koster. 1995. Gene regulation of siderophore-mediated iron acquisition in Pseudomonas: not only the Fur repressor Mol. Microbiol. 17:603-610
60. 5-oxygenase in Pseudomonas aeruginosa. J. Bacteriol. 176:1128-1140.
61. Visca, P., A. Ciervo, V. Sanfilippo, and N. Orsi. 1993. Iron-regulated salicylate synthesis by Pseudomonas spp. J. Gen Microbiol. 139:1995-2001.
62. Visca, P., L. Serino, and N. Orsi. 1992. Isolation and characterization of Pseudomonas aeruginosa mutants blocked in the synthesis of pyoverdin. J. Bacteriol. 174:5727-5731.
63. West, S. E., S. A. Kaye, A. N. Hamood, and B. H. Iglewski. 1994. Characterization of Pseudomonas aeruginosa mutants that are deficient in exotoxin A synthesis and are altered in expression of regA, a positive regulator of etotoxin A. Infect. Immun. 62:897-903.
64. Winkelmann, G. 1991. CRC handbook of microbial iron chelates CRC Press, Boca Raton, Fla
65. Wintler, H. V., and D. Haas. The homologous regulators ANR of Pseudomonas aeruginosa and FNR of Escherichia coli have overlapping but distinct specificities for anaerobically inducible promoters. Microbiology, in press.
66. Yanisch-Perron, C., J. Vieira, and J. Messing. 1985. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103-119.
67. Ye, R. W., D. Haas, J.-O. Ka, V. Krishnapillai, A. Zimerman, C. Baird, and J. M. Tiedje. 1995. Anaerobic activation of the entire denitrification pathway in Pseudomonas aeruginosa requires Anr, an analog of Fnr. J Bacteriol. 177: 3606-3609
68. E and Pseudomonas aeruginosa AlgU: E. coli rpoE restores mucoidy and reduces sensitivity to reactive oxygen intermediates in algU mutants of P. aeruginosa. J. Bacteriol. 177:3259-3268.