Construction and characterization of a Yersinia enterocolitica O:8 high-temperature requirement (htrA) isogenic mutant

Infection and Immunity

Volumn 64, Issue 6, 1996, Pages 2088-2094

  Li, Shu Rui ^a   Dorrell, Nick ^a   Everest, Paul H ^b   Dougan, Gordon ^b   Wren, Brendan W ^a  

^a ST BARTHOLOMEW S HOSPITAL   (United Kingdom)

^b IMPERIAL COLLEGE LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

GENE PRODUCT; HEAT SHOCK PROTEIN; MUTANT PROTEIN; SERINE PROTEINASE;

ARTICLE; BACTERIAL GENE; BACTERIAL VIRULENCE; GASTROENTERITIS; GRAM NEGATIVE INFECTION; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN FAMILY; SEPTICEMIA; SEQUENCE ANALYSIS; STRESS; YERSINIA ENTEROCOLITICA;

AMINO ACID SEQUENCE; ANIMALS; BACTERIAL PROTEINS; BASE SEQUENCE; HEAT-SHOCK PROTEINS; MICE; MICE, INBRED BALB C; MOLECULAR SEQUENCE DATA; MUTATION; PERIPLASMIC PROTEINS; SERINE ENDOPEPTIDASES; YERSINIA ENTEROCOLITICA;

EID: 0029947971     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/iai.64.6.2088-2094.1996     Document Type: Article

References (55)

1. Altschul, S. F., W. Gish, W. Miller, E. W. Myers, and D. J. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol. 215:403-410.
2. Badger, J. L., and V. L. Miller. 1995. Role of RpoS in survival of Yersinia enterocolitica to a variety of environmental stresses. J. Bacteriol. 177:5370-5373.
3. Baumler, A. J., J. G. Kusters, I. Slojiljkovic, and F. Heffron. 1994. Salmonella typhimurium loci involved in survival within macrophages. Infect. Immun. 62:1623-1630.
4. Bottone, E. J. 1977. Yersinia enterocolitica: a panoramic view of a charismatic organism. Crit. Rev. Microbiol. 5:211-241.
5. Bowe, F., P. O'Gaora, D. Maskell, M. Cafferkey, and G. Dougan. 1989. Virulence, persistence, and immunogenicity of Yersinia enterocolitica O:8 aroA mutants. Infect. Immun. 57:3234-3236.
6. Brenner, S. 1988. The molecular evolution of genes and proteins: a tale of two serines. Nature (London) 334:528-530.
7. Carter, P. B. 1975. Pathogenicity of Yersinia enterocolitica for mice. Infect. Immun. 11:164-170.
8. Carter, P. B., and F. M. Collins. 1974. Experimental Yersinia enterocolitica infection in mice: kinetics of growth. Infect. Immun. 9:851-857.
9. Chatfield, S. N., K. Strahan, D. Pickard, I. G. Charles, C. E. Hormaeche, and G. Dougan. 1992. Evaluation of Salmonella typhimurium strains harbouring defined mutations in htrA and aroA in the murine salmonellosis model. Microb. Pathog. 12:145-151.
10. China, B., B. T. N'Guyen, M. de Bruyere, and G. R. Cornelis. 1994. Role of YadA in resistance of Yersinia enterocolitica to phagocytosis by human polymorphonuclear leukocytes. Infect. Immun. 62:1275-1281.
11. Cornelis, G. R., T. Biot, C. Lambert de Rouvroit, T. Michiels, B. Mulder, C. Sluiters, M.-P. Sory, M. Van Bouchaute, and J. C. Vanooteghem. 1989. The Yersinia yop regulon. Mol. Microbiol. 3:1455-1459.
12. Cornelis, G. R., Y. Laroche, G. Balligand, M.-P. Sory, and G. Wauters. 1987. Yersinia enterocolitica, a primary model for bacterial invasiveness. Rev. Infect. Dis. 9:64-87.
13. Cover, T. L., and R. C. Aber. 1989. Yersinia enterocolitica. N. Engl. J. Med. 321:16-24.
14. Davies, K. J. A., and S. W. Lin. 1988. Degradation of oxidatively denatured proteins in Escherichia coli. Free Rad. Biol. Med. 5:215-233.
15. Elzer, P. H., R. W. Phillips, M. E. Kovach, K. M. Peterson, and R. M. Roop II. 1994. Characterization and genetic complementation of a Brucella abortus high-temperature-requirement A (htrA) deletion mutant. Infect. Immun. 62:4135-4139.
16. Everest, P., and G. Dougan. 1995. Unpublished data.
17. Everest, P., G. Frankel, J. Li, P. Lund, S. Chatfield, and G. Dougan. 1995. Expression of lacZ from the htrA, nirB and groE promoters in a Salmonella vaccine strain: influence of growth in mammalian cells. FEMS Microbiol. Lett. 126:97-101.
18. Forsberg, A., R. Rosqvist, and H. Wolf-Watz. 1994. Regulation and polarized transfer of the Yersinia outer proteins (Yops) involved in antiphagocytosis. Trends Microbiol. 2:15-19.
19. Forsberg, A., and H. Wolf-Watz. 1990. Genetic analysis of the yopE region of Yersinia spp.: identification of a novel conserved locus, yerA, regulating yopE expression. J. Bacteriol. 172:1547-1555.
20. Galyov, E. E., S. Hakansson, A. Forsberg, and H. Wolf-Watz. 1993. A secreted protein kinase of Yersinia pseudotuberculosis is an indispensable virulence determinant. Nature (London) 361:730-732.
21. Grinter, N. J. 1983. A broad host range cloning vector transposable to various replicons. Gene 21:133-143.
22. Hartland, E. L., S. P. Green, W. A. Phillips, and R. M. Robins-Browne. 1994 Essential role of YopD in inhibition of the respiratory hurst of macrophages by Yersinia enterocolitica. Infect. Immun. 62:4445-4453.
23. Heeseman, J., K. Gaede, and I. B. Autenrieth. 1993. Experimental Yersinia enterocolitica infection in rodents: a model for human yersiniosis. APMIS 101:417-429.
24. Holmstrom, A., R. Rosqvist, H. Wolf-Watz, and A. Forsberg. 1995. Virulence plasmid-encoded YopK is essential for Yersinia pseudotuberculosis to cause systemic infection in mice. Infect. Immun. 63:2269-2276.
25. Johnson, K., I. Charles, G. Dougan, I. Miller, D. Pickard, P. O'Gaora, G. Costa, T. Ali, and C. E. Hormaeche. 1990. The role of a stress-response protein in bacterial virulence. Res. Microbiol. 141:823-835.
26. Johnson, K., I. Charles, G. Dougan, D. Pickard, P. O'Gaora, G. Costa, T. A. I. Miller, and C. E. Hormaeche. 1991. The role of a stress-response protein in Salmonella typhimurium virulence. Mol. Microbiol. 5:401-407.
27. Kleanthous, H. 1994. Molecular studies on the pathogenicity of Helicobacter pylori. Ph.D. thesis. University of London, London.
28. Lipinska, B., O. Fayet, L. Baird, and C. Georgopoulos. 1989. Identification, characterization, and mapping of the Escherichia coli htrA gene, whose product is essential for bacterial growth only at elevated temperatures. J. Bacteriol. 171:1574-1584.
29. 32-independent mechanism of heat-inducible transcription. Nucleic Acids Res. 16:10053-10067.
30. Lipinska, B., M. Zylicz, and C. Georgopoulos. 1990. The HtrA (DegP) protein, essential for Escherichia coli survival ni high temperatures, is an endopeptidase. J. Bacteriol. 172:1791-1797.
31. Miller, V. L., and J. J. Mekalanos. 1988. A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR. J. Bacteriol. 170:2575-2583.
32. Mukkur, T. K., G. H. McDowell, B. A. Stocker, and A. K. Lascelles. 1987. Protection against experimental salmonellosis in mice and sheep by immunisation with an aromatic-dependent Salmonella typhimurium. J. Med. Microbiol. 24:11-19.
33. O'Callaghan, D., D. Maskell, F. Y. Liew, C. S. Easmon, and G. Dougan. 1988. Characterization of aromatic- and purine-dependent Salmonella typhimurium: attenuation, persistence, and ability to induce protective immunity in BALB/c mice. Infect. Immun. 56:419-423.
34. Pepe, J. C., J. L. Badger, and V. L. Miller. 1994. Growth phase and low pH affect the thermal regulation of the Yersinia enterocolitica inv gene. Mol. Microbiol. 11:123-135.
35. Pepe, J. C., M. R. Wacthel, E. Wagar, and V. L. Miller. 1995. Pathogenesis of defined invasion mutants of Yersinia enterocolitica in a BALB/c mouse model of infection. Infect. Immun. 63:4837-4848.
36. Riley, G., and S. Toma. 1989. Detection of pathogenic Yersinia enterocolitica by using Congo red-magnesium oxalate agar medium. J. Clin. Microbiol. 27:213-214.
37. Roop, R. M., II, T. W. Fletcher, N. M. Sriranganathan, S. M. Boyle, and G. G. Schurig. 1994. Identification of an immunoreactive Brucella abortus HtrA stress response protein homolog. Infect. Immun. 62:1000-1007.
38. Rosqvist, R., A. Forsberg, M. Rimpilainen, T. Bergman, and H. Wolf-Watz. 1990. The cytotoxic protein YopE of Yersinia obstructs the primary host defence. Mol. Microbiol. 4:657-667.
39. Rouslahti, E., and M. D. Pierschbacher. 1987. New perspectives in cell adhesion: RGD and adhesions. Science 238:491-497.
40. Ruiz-Bravo, A., M. Jimenez-Valera, A. Sampedro, and E. Moreno. 1994. Chromosome-mediated resistance of Yersinia enterocolitica serotype 09 to intracellular killing by mouse peritoneal macrophages. FEMS Immun. Med. Microbiol. 9:317-324.
41. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
42. See, J. H., S. K. Woo, E. M. Jung, S. Y. Yoo, C. S. Lee, K. Kim, K. Tanaka, A. Ichihara, D. B. Ha, and C. H. Chung. 1991. Protease Do is essential for survival of Escherichia coli at high temperatures: its identity with the htrA gene product. Biochem. Biophys. Res. Commun. 176:730-736.
43. Strahan, K., S. N. Chatfield, J. Tite, G. Dougan, and C. E. Hormaeche. 1992. Impaired resistance to infection does not increase the virulence of Salmonella htrA live vaccines for mice. Microb. Pathog. 12:311-317.
44. Straley, S. C., and R. Perry. 1993. Environmental modulation of gene expression and pathogenesis in Yersinia. Trends Microbiol. 3:310-319.
45. Strauch, K. L., and J. Beckwith. 1988. An Escherichia coli mutation preventing degradation of abnormal periplasmic proteins. Proc. Natl. Acad. Sci. USA 85:1576-1580.
46. Strauch, K. L., K. Johnson, and J. Beckwith. 1989. Characterization of degP, a gene required for proteolysis in the cell envelope and essential growth of Escherichia coli at high temperature. J. Bacteriol. 171:2689-2696.
47. Tatum, F. M., N. F. Cheville, and D. Morfitt. 1994. Cloning, characterization and construction of htrA and htrA-like mutants of Brucella abortus and their survival in BALB/c mice. Microb. Pathog. 17:23-36.
48. Une, T. 1977. Studies on the pathogenicity of Yersinia enterocolitica. I. Experimental infection in rabbits. Microbiol. Immunol. 21:349-363.
49. Une, T. 1977. Studies on the pathogenicity of Yersinia enterocolitica. II. Interaction with cultured cells in vitro. Microbiol. Immunol. 21:364-377.
50. Wierenga, R. K., and W. G. J. Hol. 1983. Predicted nucleotide-binding properties of p21 protein and its cancer related variant. Nature (London) 304:842-844.
51. Wren, B.W., S. M. Colby, R. R. Cubberley, and M. J. Pallen. 1992. Degenerate PCR primers for the amplification of fragments from genes encoding response regulators from a range of pathogenic bacteria. FEMS Microbiol. Lett. 78:287-291.
52. Wren, B. W., J. Henderson, and J. M. Ketley. 1994. A PCR-based strategy for the rapid construction of defined bacterial deletion mutants. BioTechniques 16:994-996.
53. Wren, B. W., J. Henderson, and J. M. Ketley. Unpublished data
54. Wren, B. W., and S. Tabaqchali. 1990. Detection of pathogenic Yersinia enterocolitica by the polymerase chain reaction. Lancet 336:693.
55. + mutant of Yersinia enterocolitica serotype O:8 and its application in construction of rough mutants utilizing mini-Tn5 derivatives and lipopolysaccharide-specific phage. J. Bacteriol. 176:1756-1760.