Use of lysolecithin-permeabilized infected-cell extracts to investigate the in vitro biochemical phenotypes of poxvirus ts mutations altered in viral transcription activity

Virology

Volumn 218, Issue 1, 1996, Pages 169-180

  Condit, Richard C ^a   Lewis, Jackie I ^a   Quinn, Michelle ^b   Christen, Linda M ^b   Niles, Edward G ^b  

^a UNIVERSITY OF FLORIDA   (United States)

^b UNIVERSITY AT BUFFALO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

LYSOPHOSPHATIDYLCHOLINE;

ARTICLE; BIOCHEMISTRY; CONTROLLED STUDY; GENE EXPRESSION REGULATION; NONHUMAN; PHENOTYPE; PLASMID; POXVIRUS; PRIORITY JOURNAL; TEMPERATURE SENSITIVE MUTANT; TRANSCRIPTION REGULATION; VACCINIA VIRUS; VIRUS MUTANT;

EID: 0029947394     PISSN: 00426822     EISSN: None     Source Type: Journal    
DOI: 10.1006/viro.1996.0177     Document Type: Article

References (68)

1. Ahn, B. Y., and Moss, B. (1992). RNA polymerase-associated transcription specificity factor encoded by vaccinia virus. Proc. Natl. Acad. Sci. USA 89, 3536-3540.
2. Ahn, B. Y., Gershon, P. D., and Moss, B. (1994). Rap 94 confers promoter specificity for initiating transcription of vaccinia virus early stage genes. J. Biol. Chem. 269, 7552-7557.
3. Baldick, C. J., and Moss, B. (1993). Characterization and temporal regulation of mRNAs encoded by vaccinia virus intermediate-stage genes. J. Virol. 67, 3515-3527.
4. Baldick, C. J., Jr., Keck, J. G., and Moss, B. (1992). Mutational analysis of the core, spacer, and initiator regions of vaccinia virus intermediate-class promoters. J. Virol. 66, 4710-4719.
5. Baroudy, B. M., and Moss, B. (1980). Purification and characterization of a DNA-dependent RNA polymerase from vaccinia virus. J. Biol. Chem. 255, 4372-4380.
6. Black, E. P., and Condit, R. C. (1996). Phenotypic characterization of mutants in vaccinia virus gene g2r, a putative transcription elongation factor. J. Virol. 70G, 47-54.
7. Brown, G. D., Peluso, R. W., Moyer, S. A., and Moyer, R. W. (1983). A simple method for the preparation of extracts from animal cells which catalyze efficient in vitro protein synthesis. J. Biol. Chem. 258, 14309-14314.
8. Broyles, S., and Fesler, B. (1990). Vaccinia virus gene encoding a component of the viral early transcription factor. J. Virol. 64, 1523-1529.
9. Broyles, S. S., and Moss, B. (1986). Homology between RNA polymerases of poxviruses, prokaryotes, and eukaryotes: Nucleotide sequence and transcriptional analysis of vaccinia virus genes encoding 147-kDa and 22-kDa subunits. Proc. Natl. Acad. Sci. USA 83, 3141-3145.
10. Broyles, S. S., Li, J., and Moss, B. (1991). Promoter DNA contacts made by the vaccinia virus early transcription factor. J. Biol. Chem. 266, 15539-15544.
11. Broyles, S. S., Yuen, L., Shuman, S., and Moss, B. (1988). Purification of a factor required for transcription of vaccinia virus early genes. J. Biol. Chem. 263, 10754-10760.
12. Carlsen, S. R., Peluso, R. W., and Moyer, S. A. (1985). In vitro replication of Sendai virus wild-type and defective interfering particle genome RNAs. J. Virol. 54, 493-500.
13. Carpenter, M. S., and DeLange, A. M. (1991). A temperature-sensitive lesion in the small subunit of the vaccinia virus-encoded mRNA capping enzyme causes a defect in viral telomere resolution. J. Virol. 65, 4042-4050.
14. Carpenter, M. S., and Delange, A. M. (1992). Identification of a temperature-sensitive mutant of vaccinia virus defective in late but not intermediate gene expression. Virology 188, 233-244.
15. Christen, L., Higman, M. A., and Niles, E. G. (1992). Phenotypic characterization of three temperature-sensitive mutations in the vaccinia virus early gene transcription initiation factor. J. Gen. Virol. 73, 3155-3167.
16. Condit, R. C., and Motyczka, A. (1981). Isolation and preliminary characterization of temperature-sensitive mutants of vaccinia virus. Virology 113, 224-241.
17. Condit, R. C., Motyczka, A., and Spizz, G. (1983). Isolation, characterization, and physical mapping of temperature-sensitive mutants of vaccinia virus. Virology 128, 429-443.
18. Dales, S., Milovanivitch, V., Pogo, B., Weintraub, S., Huima, T., Wilton, S., and McFadden, G. (1978). Biogenesis of vaccinia: Isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84, 403-428.
19. Deng, L., and Shuman, S. (1994). A role for the H4 subunit of vaccinia RNA polymerase in transcription initiation at a viral early promoter. J. Biol. Chem. 269, 14323-14329.
20. Oyster, L. M., and Niles, E. G. (1991). Genetic and biochemical characterization of vaccinia virus genes D2L and D3R which encode virion structural proteins. Virology 182, 455-467.
21. Ensinger, M. J. (1982). Isolation and genetic characterization of temperature-sensitive mutants of vaccinia virus WR. J. Virol. 43, 778-790.
22. r subunit of the virion-associated DNA-dependent RNA polymerase. J. Virol. 61, 1842-1850.
23. Ensinger, M. J., and Rovinsky, M. (1983). Marker rescue of temperature-sensitive mutations of vaccinia virus WR: Correlation of genetic and physical maps. J. Virol. 48, 419-428.
24. Foglesong, P. D. (1985). In vitro transcription of a cloned vaccinia virus gene by a soluble extract prepared from vaccinia virus-infected HeLa cells. J. Virol. 53, 822-826.
25. Gershon, P. D., and Moss, B. (1990). Early transcription factor subunits are encoded by vaccinia virus late genes. Proc. Natl. Acad. Sci. USA 87, 4401-4405.
26. Goebel, S. J., Johnson, G. P., Perkus, M. E., Davis, S. W., Winslow, J. P., and Paoletti, E. (1990). The complete DNA sequence of vaccinia virus. Virology 179, 247-266.
27. Hagler, J., and Shuman, S. (1992). Ternary complex formation by vaccinia virus RNA polymerase at an early viral promoter: Analysis by native gel electrophoresis. J. Virol. 66, 2982-2989.
28. Hagler, J., Luo, Y., and Shuman, S. (1994). Factor-dependent transcription termination by vaccinia RNA polymerase: Kinetic coupling and requirement for ATP hydrolysis. J. Biol. Chem. 269, 10050-10060.
29. Harris, N., Rosales, R., and Moss, B. (1993). Transcription initiation factor activity of vaccinia virus capping enzyme is independent of mRNA guanylylation. Proc. Natl. Acad. Sci. USA 90, 2860-2864.
30. Hassett, D. E., and Condit, R. C. (1994). Targeted construction of temperature sensitive mutations in vaccinia virus using clustered charge to alanine mutagenesis. Proc. Natl. Acad. Sci. USA 91, 4554-4558.
31. Higman, M. A., and Niles, E. G. (1992). The vaccinia virus (guanine-7-)methyltransferase requires both subunits of the mRNA capping enzyme for activity. J. Biol. Chem. 267, 16430-16437.
32. Higman, M. A., Christen, L. A., and Niles, E. G. (1994). The mRNA (guanine-7-)methyltransferase domain of the vaccinia virus mRNA capping enzyme: Expression in Escherichia coli and structural and kinetic comparison to the intact capping enzyme. J. Biol. Chem. 269.
33. Hooda-Dhingra, U., Patel, D., Pickup, D. J., and Condit, R. C. (1990). Fine structure mapping and phenotypic analysis of five temperature-sensitive mutations in the second largest subunit of vaccinia virus DNA-dependent RNA polymerase. Virology 174, 60-69.
34. Hooda-Dhingra, U., Thompson, C. L., and Condit, R. C. (1989). Detailed phenotypic characterization of five temperature sensitive mutants in the 22 kilodalton and the 147 kilodalton subunits of the vaccinia virus DNA-dependent RNA polymerase. J. Virol. 63, 714-729.
35. Horikami, S. M., and Moyer, S. A. (1991). Synthesis of leader RNA and editing of the P mRNA during transcription by purified measles virus. J. Virol. 65, 5342-5347.
36. Horikami, S. M., Curran, J., Kolakofsky, D., and Moyer, S. A. (1992). Complexes of Sendai virus NP-P and P-L proteins are required for defective interfering particle genome replication in vitro. J. Virol. 66, 4901-4908.
37. Kane, E. M., and Shuman, S. (1992). Temperature-sensitive mutations in the vaccinia virus H4 gene encoding a component of the virion RNA polymerase. J. Virol. 66, 5752-5762.
38. Keck, J. G., Baldick, C. J. J., and Moss, B. (1990). Role of DNA replication in vaccinia virus gene expression: A naked template is required for transcription of three late trans-activator genes. Cell 61, 801-809.
39. Kovacs, G. R., Rosales, R., Keck, J. G., and Moss, G. (1994). Modification of the cascade model for regulation of vaccinia virus gene expression: Purification of a pre-replicative, late-stage specific transcription factor. J. Virol. 68, 3443-3447.
40. Luo, Y., Hagler, J., and Shuman, S. (1991). Discrete functional stages of vaccinia virus early transcription during a single round of RNA synthesis in vitro. J. Biol. Chem. 266, 13303-13310.
41. Luo, Y., Mao, X., Deng, L., Cong, P., and Shuman, S. (1995). The D1 and D12 subunits are both essential for transcription termination factor activity of the vaccinia virus capping enzyme. J. Virol. 69, 3852-3856.
42. Mao, X., and Shuman, S. (1994). Intrinsic (guanine-7-)methyltransferase activity of the vaccinia virus capping enzyme D1 subunit is stimulated by the D12 subunit: Identification of amino acid residues in the D1 protein required for subunit association and methyl group transfer. J. Biol. Chem. 269, 24472-24479.
43. Moss, B. (1990a). Poxviridae and their replication. In "Virology," 2nd ed. (B. N. Fields, D. M. Knipe, et al., Eds.), pp. 2079-2111. Raven Press, New York.
44. Moss, B. (1990b). Regulation of vaccinia virus transcription. Annu. Rev. Biochem. 59, 661-688.
45. Moyer, S. A., Baker, S. C., and Horikami, S. M. (1990). Host cell proteins required for measles virus reproduction. J. Gen. Virol. 71, 775-783.
46. Myette, J. R., and Niles, E. G. (1996). Domain structure of the vaccinia virus mRNA capping enzyme: Expression in E. coli of a subdomain possessing the mRNA triphosphatase and guanylyltransferase activities and a kinetic comparison to the full-size enzyme. Submitted for publication.
47. Nevins, J. R., and Joklik, W. K. (1977). Isolation and properties of the vaccinia virus DNA-dependent RNA polymerase. J. Biol. Chem. 252, 6930-6938.
48. Niles, E. G., Christen, L. M., and Higman, M. A. (1994). Direct photolinkage of GTP to the vaccinia virus mRNA (guanine-7)methyltransferase GTP methyl acceptor site. Biochemistry 33, 9898-9903.
49. Niles, E. G., Lee-Chen, G. J., Shuman, S., Moss, B., and Broyles, S. S. (1989). Vaccinia virus gene D12L encodes the small subunit of the viral mRNA capping enzyme. Virology 172, 513-522.
50. Peluso, R. W., and Moyer, S. A. (1983). Initiation and replication of vesicular stomatitis virus genome RNA in a cell-free system. Proc. Natl. Acad. Sci. USA 80, 3198-3202.
51. Puckett, C., and Moss, B. (1983). Selective transcription of vaccinia virus genes in template dependent soluble extracts of infected cells. Cell 35, 441-448.
52. Rosales, R., Harris, N., Ahn, B. Y., Keck, J. G., and Moss, B. (1994). Purification and identification of a vaccinia virus-encoded intermediate stage promoter-specific transcription factor that has homology to eukaryotic transcription factor SII (TFIIS) and an additional role as a viral RNA polymerase subunit. J. Biol. Chem. 269, 14260-14267.
53. Sawadogo, M., and Roeder, R. G. (1985). Factors involved in specific transcription by human RNA polymerase II: Analysis by a rapid and quantitative in vitro assay. Proc. Natl. Acad. Sci. USA 82, 4394-4398.
54. Schwer, B., and Stunnenberg, H. G. (1988). Vaccinia virus late transcripts generated in vitro have a poly(A) head. EMBO J. 7, 1183-1190.
55. Shuman, S. (1989). Functional domains of the vaccinia virus mRNA capping enzyme. J. Biol. Chem. 264, 9690-9695.
56. Shuman, S. (1990). Catalytic activity of vaccinia mRNA capping enzyme subunits coexpressed in Escherichia coli. J. Biol. Chem. 265, 11960-11966.
57. r 95,000 subunit expressed in Escherichia coli. J. Biol. Chem. 265, 11967-11972.
58. Shuman, S., Broyles, S. S., and Moss, B. (1987). Purification and characterization of a transcription termination factor from vaccinia virions. J. Biol. Chem. 262, 12372-12380.
59. Simpson, D. A., and Condit, R. C. (1994). The vaccinia virus A18Rprotein plays a role in viral transcription during both the early and late phases of infection. J. Virol. 68, 3642-3649.
60. Thompson, C. L., Hooda-Dhingra, U., and Condit, R. C. (1989). Fine structure mapping of five temperature sensitive mutations in the 22 kilodalton and the 147 kilodalton subunits of the vaccinia virus DNA-dependent RNA polymerase. J. Virol. 63, 705-713.
61. Vos, J. C., and Stunnenberg, H. G. (1988). Derepression of a novel class of vaccinia virus genes upon DNA replication. EMBO J. 7, 3487-3492.
62. Vos, J. C., Sasker, M., and Stunnenberg, H. G. (1991). Vaccinia virus capping enzyme is a transcription initiation factor. EMBO J. 10, 2553-2558.
63. Wittek, R., Cooper, J. A., Barbosa, E., and Moss, B. (1980). Expression of the vaccinia virus genome: Analysis and mapping of mRNAs encoded within the inverted terminal repetition. Cell 21, 487-493.
64. Wright, C. F., and Coroneos, A. M. (1995). The H4 subunit of vaccinia virus RNA polymerase is not required for transcription initiation at a viral late promoter. J. Virol. 69, 2602-2604.
65. Wright, C. F., and Moss, B. (1989). Identification of factors specific for transcription of the late class of vaccinia virus genes. J. Virol. 63, 4224-4233.
66. Wright, D. F., and Coroneos, A. M. (1993). Purification of the late transcription system of vaccinia virus: Identification of a novel transcription factor. J. Virol. 67, 7264-7270.
67. Zhang, Y., Ahn, B. Y., and Moss, B. (1994). Targeting of a multicomponent transcription apparatus into assembling vaccinia virus particles requires RAP94, an RNA polymerase-associated protein. J. Virol. 68, 1360-1370.
68. Zhang, Y., Keck, J. G., and Moss, B. (1992). Transcription of viral late genes is dependent on expression of the viral intermediate gene G8R in cells infected with an inducible conditional-lethal mutant vaccinia virus. J. Virol. 66, 6470-6479.