Characterization of a Penicillium chrysogenum gene encoding a PacC transcription factor and its binding sites in the divergent pcbAB-pcbC promoter of the penicillin biosynthetic cluster

Molecular Microbiology

Volumn 20, Issue 3, 1996, Pages 529-540

  Suárez, Teresa ^a   Peñalva, Miguel Angel ^a  

^a CENTRO DE INVESTIGACIONES BIOLÓGICAS   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

TRANSCRIPTION FACTOR;

ARTICLE; ASPERGILLUS; BIOSYNTHESIS; CONTROLLED STUDY; DNA BINDING; DNA SEQUENCE; GENE CLUSTER; GENE EXPRESSION REGULATION; NONHUMAN; PENICILLIUM CHRYSOGENUM; PH; PRIORITY JOURNAL; PROMOTER REGION; TRANSCRIPTION REGULATION;

EID: 0029947259     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1996.5421065.x     Document Type: Article

References (25)

1. Arst, Jr, H.N., Bignell, E., and Tilburn, J. (1994) Two new genes involved in signalling ambient pH in Aspergillus nidulans. Mol Gen Genet 245: 787-790.
2. Backus, M.P., and Stauffer, J.F. (1955) The production and selection of a familiy of strains in Penicillium chrysogenum. Mycologia 47: 275-428.
3. Caddick, M.X., Brownlee, A.G., and Arst, Jr, H.N. (1986) Regulation of gene expression by pH of the growth medium in Aspergillus nidulans. Mol Gen Genet 203: 346-353.
4. Cove, D.J. (1966) The induction and repression of nitrate reductase in the fungus Aspergillus nidulans. Biochim Biophys Acta 113: 51-56.
5. Chu, Y., Renno, D., and Saunders, G. (1995) Detection of a protein which binds specifically to the upstream region of the pcbAB gene in Penicillium chrysogenum. Curr Genet 27: 184-189.
6. Denison, S.H., Orejas, M., and Arst, Jr, H.N. (1995) Signaling of ambient pH in Aspergillus involves a cysteine protease. J Biol Chem 270: 28519-28522.
7. Espeso, E.A. (1994) Control por genes reguladores de amplio dominio de la biosíntesis de penicilina en Aspergillus nidulans. PhD Thesis, Universidad Complutense, Madrid.
8. Espeso, E.A., and Peñalva, M.A. (1992) Carbon catabolite repression can account for the temporal pattern of expression of a penicillin biosynthetic gene in Aspergillus nidulans. Mol Microbiol 6: 1457-1465.
9. Espeso, E.A., and Peñalva, M.A. (1994) In vitro binding of the two-finger repressor CreA to several consensus and non-consensus sites at the ipnA upstream region is context dependent. FEBS Lett 342: 43-48.
10. Espeso, E.A., Tilburn, J., Arst, Jr, H.N., and Peñalva, M.A. (1993) pH regulation is a major determinant in expression of a fungal penicillin biosynthetic gene. EMBO J 12: 3947-3956.
11. Feinberg, B.P., and Vogelstein, B. (1984) A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 137: 266-267.
12. Feng, B., Friedlin, E., and Marzluf, G. (1995) Nuclear DNA-binding proteins which recognize the intergenic control region of penicillin biosynthetic genes. Curr Genet 27: 351-358.
13. Gómez-Pardo, E., and Peñalva, M.A. (1990) The upstream region of the IPNS gene determines expression during secondary metabolism in Aspergillus nidulans. Gene 89: 109-115.
14. Lockington, R.A., Sealy-Lewis, H.M., Scazzocchio, C., and Davis, R.W. (1985) Cloning and characterisation of the ethanol utilisation regulon in Aspergillus nidulans. Gene 33: 137-149.
15. MacCabe, A.P., van den Hombergh, J.P.T.W., Tilburn, J., Arst, Jr, H.N., and Visser, J. (1996) Identification, cloning and analysis of the Aspergillus niger pacC wide domain pH regulatory gene. Mol Gen Genet 250: 367-374.
16. Orejas, M., Espeso, E.A., Tilburn, J., Sarkar, S., Arst, Jr, H.N., and Peñalva, M.A. (1995) Activation of the Aspergillus PacC transcription factor in response to alkaline ambient pH requires proteolysis of the carboxy-terminal moiety. Genes Dev 9: 1622-1632.
17. Peñalva, M.A., Moya, A., Dopazo, J., and Ramón, D. (1990) Sequences of isopenicillin N synthetase genes suggest horizontal gene transfer from prokaryotes to eukaryotes. Proc Roy Soc Lond B, 241: 164-169.
18. Pérez-Esteban, B., Orejas, M., Gómez Pardo, E., and Peñalva, M.A. (1993) Molecular characterization of a fungal secondary metabolism promoter: transcription of the Aspergillus nidulans isopenicillin N synthetase gene is modulated by upstream negative elements. Mol Microbiol 9: 881-895.
19. Raper, K.B., and Alexander, D.F. (1945) Penicillin. V. Microbiological aspects of penicillin production. J Elisha Mitch Sci Soc 61: 74-113.
20. Revilla, G., López-Nieto, M.J., Luengo, J.M., and Martín, J.F. (1984) Carbon catabolite repression of penicillin biosynthesis by Penicillium chrysogenum. J Antibiot 37: 781-789.
21. Revilla, G., Ramos, F.R., López-Nieto, M.J., Alvarez, E., and Martín, J.F. (1986) Glucose represses formation of δ-(L-α-aminoadipyl)-L-cysteinyl-D-valine and isopenicillin N synthase but not penicillin acyltransferase in Penicillium chrysogenum. J Bacteriol 168: 947-952.
22. Shah, A.J., Tilburn, J., Adlard, M.W., and Arst, Jr, H.N. (1991) pH regulation of penicillin production in Aspergillus nidulans. FEMS Microbiol Lett 77: 209-212.
23. Smith, D.J., Earl, A.J., and Turner, G. (1990) The multifunctional peptide synthetase performing the first step of penicillin biosynthesis in Penicillium chrysogenum is a 421073 dalton protein similar to Bacillus brevis peptide antibiotic biosynthesis. EMBO J 9: 2743-2750.
24. Tilburn, J., Scazzocchio, C., Taylor, G.G., Zabicky-Zissman, J.H., Lockington, R.A., and Davies, R.W. (1983) Transformation by integration in Aspergillus nidulans. Gene 26: 205-211.
25. Tilburn, J., Sarkar, S., Widdick, D.A., Espeso, E.A., Orejas, M., Mungroo, J., Peñalva, M.A., and Arst, Jr, H.N. (1995) The Aspergillus PacC zinc finger transcription factor mediates regulation of both acid- and alkaline-expressed genes by ambient pH. EMBO J 14: 779-790.