Expression and characterization of functionally active recombinant perforin produced in insect cells

Journal of Immunology

Volumn 156, Issue 9, 1996, Pages 3292-3300

  Liu, Chau Ching ^a   Persechini, Pedro M ^b   Young, John Ding E ^a  

^a ROCKEFELLER UNIVERSITY   (United States)

^b FEDERAL UNIVERSITY OF RIO DE JANEIRO   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM; CHROMIUM 51; DNA; PERFORIN; RECOMBINANT PROTEIN;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; CALCIUM TRANSPORT; CELL LINE; CONTROLLED STUDY; CYTOLYSIS; ERYTHROLEUKEMIA CELL; HEMOLYSIS; HUMAN; HUMAN CELL; INSECT; LYMPHOKINE ACTIVATED KILLER CELL; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS;

ANIMALS; BACULOVIRIDAE; CYTOTOXICITY, IMMUNOLOGIC; GENETIC VECTORS; HUMANS; LEUKEMIA, ERYTHROBLASTIC, ACUTE; LYMPHOMA; MAST-CELL SARCOMA; MEMBRANE GLYCOPROTEINS; MICE; PORE FORMING CYTOTOXIC PROTEINS; RECOMBINANT PROTEINS; SPODOPTERA; TUMOR CELLS, CULTURED;

EID: 0029946040     PISSN: 00221767     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (54)

1. Henkart, P. A. 1985. Mechanism of lymphocyte-mediated cytotoxicity. Annu. Rev. Immunol. 3:31.
2. Liu, C.-C., C. M. Walsh, and J. D.-E. Young. 1995. Perforin: structure and function. Immunol. Today 16:194.
3. Podack, E. R. 1995. Execution and suicide: cytotoxic lymphocytes enforce Draconian laws through separate molecular pathways. Curr. Opin. Immunol. 7:11.
4. Podack, E. R., J. D.-E. Young, and Z. A. Conn. 1985. Isolation and biochemical and functional characterization of perforin 1 from cytolytic T-cell granules. Proc. Natl. Acad. Sci. USA 82:8629.
5. Masson, D., and J. Tschopp. 1985. Isolation of a lytic, pore-forming protein (perforin) from cytolytic T-lymphocytes. J. Biol. Chem. 260:9069.
6. Young, J. D.-E., H. Hengartner, E. R. Podack, and Z. A. Cohn. 1986. Purification and characterization of a cytolytic pore-forming protein from granules of cloned lymphocytes with natural killer activity. Cell 44:849.
7. Young, J. D.-E., C.-C. Liu, P. M. Persechini, and Z. A. Cohn. 1988. Perforin-dependent and -independent pathways of cytotoxicity mediated by lymphocytes. Immunol. Rev. 103:161.
8. Tschopp, J., and M. Nabholz. 1990. Perforin-mediated target cell lysis by cytolytic T lymphocytes. Annu. Rev. Immunol. 8:279.
9. Podack, E. R., H. Hegartner, and M. G. Lichtenheld. 1991. A central role of perforin in cytolysis? Annu. Rev. Immunol. 9:129.
10. Young, J. D.-E., Z. A. Cohn, and E. R. Podack. 1986. The ninth component of complement and the pore-forming protein (perforin 1) from cytotoxic T cells: structural, immunological, and functional similarities. Science 233:184.
11. Shinkai, Y., K. Takio, and K. Okumura. 1988. Homology of perforin to the ninth component of complement (C9). Nature 334:525.
12. Tschopp, J., D. Masson, and K. K. Stanley. 1986. Structural/functional similarity between proteins involved in complement- and cytotoxic T-lymphocyte-mediated cytolysis. Nature 322:831.
13. Young, J. D.-E., C.-C. Liu, L. G. Leong, and Z. A. Cohn. 1986. The pore-forming protein (perforin) of cytolytic T lymphocytes is immunologically related to the components of membrane attack complex of complement through cysteine-rich domains. J. Exp. Med. 164:2077.
14. Muller-Eberhard, H. J. 1986. The membrane attack complex of complement. Annu. Rev. Immunol. 4:503.
15. Lichtenheld, M. G., K. Olsen, P. Lu, D. M. Lowrey, A. Hameed, H. Hengartner, and E. R. Podack. 1988. Structure and function of human perforin. Nature 335: 448.
16. Lowrey, D. M., T. Aebischer, K. Olsen, M. Lichtenheld, F. Rupp, H. Hengartner, and E. R. Podack. 1989. Cloning, analysis, and expression of murine perforin 1 cDNA, a component of cytolytic T-cell granules with homology to complement component C9. Proc. Natl. Acad. Sci. USA 86:247.
17. Kwon, B. S., M. Wakulchik, C.-C. Liu, P. M. Persechini, J. Trapani, A. K. Haq, Y. Kim, and J. D.-E. Young. 1989. The structure of the lymphocyte pore-forming protein perforin. Biochem. Biophys. Res. Commun. 158:1.
18. Ojcius, D. M., P. M. Persechini, L. M. Zheng, P. C. Notaroberto, S. C. Adeodato, and J. D.-E. Young. 1991. Cytolytic and ion channel-forming properties of the N terminus of lymphocyte perforin. Proc. Natl. Acad. Sci. USA 88:4621.
19. Persechini, P. M., D. M. Ojcius, S. C. Adeodato, P. C. Notaroberto, C. B. Daniel, and J. D.-E. Young. 1992. Channel-forming activity of the perforin N-terminus and a putative alpha-helical region homologous with complement C9. Biochemistry 31:5017.
20. Hink, W. F. 1970. Established insect cell line from the cabbage looper, Trichoplusia ni. Nature 226:466.
21. Walsh, C. M., M. Matloubian, C.-C. Liu, R. Ueda, C. G. Kurahara, J. L. Christensen, M. T. F. Huang, J. D.-E. Young, R. Ahmed, and W. R. Clark. 1994. Immune function in mice lacking the perform gene. Proc. Natl. Acad. Sci. USA 91:10854.
22. Liu, C.-C., C. M. Walsh, N. Eto, W. R. Clark, and J. D.-E. Young. 1995. Morphological and functional characterization of perforin-deficient lymphokine-activated killer cells. J. Immunol. 155:602
23. Summers, M. D., and G. E. Smith. 1987. A Manual of Methods for Baculovirus Vectors and Insect Cell Culture Procedures. Texas Agricultural Experiment Station Bulletin No. 1555.
24. Parr, E. L., L. H. Y. Young, M. B. Parr, and J. D.-E. Young. 1990. Granulated metrial gland cells of pregnant mouse uterus are NK-like cells that contain perform and serine esterases. J. Immunol. 145:2365.
25. Liu, C.-C., P. M. Persechini, and J. D.-E. Young. 1994. Characterization of recombinant mouse perforin expressed in insect cells using the baculovirus system. Biochem. Biophys. Res. Commun. 201:318.
26. Zheng, L. M., D. M. Ojcius, C.-C. Liu, M. D. Kramer, M. M. Simon, E. L. Parr, and J. D.-E. Young. 1991. Immunogold labelling of perforin and serine esterases in granulated metrial gland cells. FASEB J. 5:79.
27. Koizumi, H., C.-C. Liu, L. M. Zheng, S. V. Joag, N. K. Bayne, J. Holoshitz, and J. D.-E. Young. 1991. Expression of perforin and serine esterases by human (gamma/delta) T-cells. J. Exp. Med. 173:499.
28. Podack, E. R., and P. J. Konigsberg. 1984. Cytolytic T cell granules: isolation, structural, biochemical, and functional characterization. J. Exp. Med. 160:695.
29. Persechini, P. M., and J. D.-E. Young. 1988. The primary structure of the lymphocyte pore-forming protein perforin: partial amino acid sequencing and determination of isoelectric point. Biochem. Biophys. Res. Commun. 156:740.
30. Duke, R. C., P. M. Persechini, S. Chang, C.-C. Liu, J. Cohen, and J. D.-E. Young. 1989. Purified perforin induces target cell lysis but not DNA fragmentation. J. Exp. Med. 170:1451.
31. Ojcius, D. M., L. M. Zheng, E. C. Sphicas, A. Zychlinsky, and J. D.-E. Young. 1991. Subcellular localization of perforin and serine esterase in lymphokine-activated killer cells and cytotoxic T cells by immunogold labeling. J. Immunol. 146:4427.
32. Young, J. D.-E., A. Damiano, M. A. DiNome, L. G. Leong, and Z. A. Cohn. 1987. Dissociation of membrane binding and lytic activities of the lymphocyte pore-forming protein (perforin). J. Exp. Med. 165:1371.
33. Persechini, P. M., J. D.-E. Young, and W. Almers. 1990. Membrane channel formation by the lymphocyte pore-forming protein: comparision between susceptible and resistant target cells. J. Cell Biol. 110:2109.
34. Zheng, L. M., A. Zychlinsky, C.-C. Liu, D. M. Ojcius, and J. D.-E. Young. 1991. Extracellular ATP as a trigger for apoptosis or programmed cell death. J. Cell Biol. 112:279.
35. Shi, L., R. P. Kraut, R. Aebersold, and A. H. Greenberg. 1992. A natural killer cell granule protein that induces DNA fragmentation and apoptosis. J. Exp. Med. 175:553.
36. Shi, L., C.-M. Kam, J. C. Powers, R. Aebersold, and A. H. Greenberg. 1992. Purification of three cytotoxic lymphocyte granule serine proteases that induce apoptosis through distinct substrate and target cell interaction. J. Exp. Med. 176: 1521.
37. Luckow, V. A., and M. D. Summers. 1988. Trends in the development of baculovirus expression vectors. Biotechnology 6:47.
38. Possee, R. D. 1986. Cell-surface expression of influenza virus hemagglutinin in insect cells using a baculovirus vector. Virus Res. 5:43.
39. Wojchowski, D. M., S. H. Orkin, and A. J. Sytkowski. 1987. Active human erythropoietin expressed in insect cells using a baculovirus vector: a role for N-linked oligosaccharide. Biochem. Biophys. Acta 910:224.
40. Greenfield, C., G. Patel, S. Clark, N. Jones, and M. D. Waterfleld. 1988. Expression of the human EGF receptor with ligand-stimulatable kinase activity in insect cells using a baculovirus vector. EMBO J. 7:139.
41. Jarvis, D. L., and M. D. Summers. 1989. Glycosylation and secretion of human tissue plasminogen activator in recombinant baculovirus-infected insect cells. Mol. Cell. Biol. 9:214.
42. Kontermann, R., and E. W. Rauterberg. 1989. N-deglycosylation of human complement component C9 reduces its hemolytic activity. Mol. Immunol. 26:1125.
43. Parekh, R. B. 1991. Mammalian cell gene expression: protein glycosylation. Curr. Opin. Biotechnol. 2:730.
44. Jarvis, D. L., C. Oker-Blom, and M. D. Summers. 1990. Role of glycosylation in the transport of recombinant glycoproteins through the secretory pathway of lepidopteran insect cells. J. Cell. Biochem. 42:181.
45. Miyajima, A., J. Schreurs, K. Otsu, A. Kondo, K. Arai, and S. Maeda. 1987. Use of the silkworm, Bombyx mori, and an insect baculovirus vector for high-level expression and secretion of biologically active mouse interleukin-3. Gene 58:278.
46. Knops, J., K. Johnson-Wood, D. B. Schenk, S. Sinha, I. Lieberburg, and L. McConlogue. 1991. Isolation of baculovirus-derived secreted and full-length beta-amyloid precursor protein. J. Biol. Chem. 266:7285.
47. Rodewald, H. R., J. Langhorne, K. Eichmann, and J. Kupsch. 1904. Production of murine interleukin-4 and interleukin-5 by recombinant baculovirus. J. Immunol. Methods 132:221.
48. Tomlinson, S., E. Ueda, J. E. Maruniak, A. Garcia-Canedo, E. S. Bjes, and A. F. Esser. 1993. The expression of hemolytically active human complement protein C9 in mammalian, insect, and yeast cells. Protein Expr. Purif. 4:141.
49. Webb, E., J. Tkalcevic, S. Edwards, D. Hocking, and I. Nisbet. 1993. Expression of biologically active human factor VIII using a baculovirus vector. Biochem. Biophys. Res. Commun. 190:536.
50. Grabenhorst, E., B. Hofer, M. Nimtz, V. Jager, and H. S. Conradt. 1993. Biosynthesis and secretion of human interleukin 2 glycoprotein variants from baculovirus-infected Sf21 cells: characterization of polypeptides and post-translational modification. Eur. J. Biochem. 215:189.
51. Peakman, T. C., I. G. Charles, M. A. Sydenham, D. R. Gewert, M. J. Page, and J. Makoff. 1992. Enhanced expression of recombinant proteins in insect cells using a baculovirus vector containing a bacterial leader sequence. Nucleic Acids Res. 20:6111.
52. Daugherty, B. L., S. M. Zavodny, A. B. Lenny, M. A. Jacobson, R. W. Ellis, S. W. Law, and G. E. Mark. 1990. The uses of computer-aided signal peptide selection and polymerase chain reaction in gene construction and expression of secreted proteins. DNA Cell Biol. 9:453.
53. Peitsch, M. C., P. Amiguet, R. Guy, J. Brunner, J. V. Maizel, Jr., and J. Tschopp. 1990. Localization and molecular modelling of the membrane-inserted domain of the ninth component of human complement and perforin. Mol. Immunol. 27:589.
54. Lowin, B., O. Krahenbuhl, C. Muller, M. Dupuis, and J. Tschopp. 1992. Perforin and its role in T lymphocyte-mediated cytolysis. Experientia 48:911.