Stable isotope incorporation evidence for the De Novo biosynthesis of terpenoic acid glycerides by dorid nudibranchs

Tetrahedron

Volumn 52, Issue 20, 1996, Pages 6869-6878

  Graziani, Edmund I ^a   Andersen, Raymond J ^a   Krug, Patrick J ^b   Faulkner, D John ^b  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACYLGLYCEROL; CARBON 13; TANYOLIDE B; TERPENOID; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ARTICLE; BIOSYNTHESIS; CONTROLLED STUDY; INVERTEBRATE; ISOTOPE LABELING; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; PRIORITY JOURNAL;

EID: 0029944543     PISSN: 00404020     EISSN: None     Source Type: Journal    
DOI: 10.1016/0040-4020(96)00327-4     Document Type: Article

References (20)

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18. For a precedent see: Needham, J.; Hu, T.; McLachlan, J.; Walter, J.; Wright, J. J. Chem. Soc., Chem. Commun. 1995, 1623.
19. Numbers listed for specific incorporations = % enrichments above natural abundance = 1.1% X (combined integrated peak area of enriched satellites minus the combined theoretical peak area for these same satellites resulting from natural abundance coupling)/(peak height of the natural abundance singlet plus the combined theoretical peak area for all satellites resulting from natural abundance coupling). The probability of observing natural abundance coupling between a pair of adjacent carbons is 0.011 × 0.011 = 0.000121. The intensity of this signal would be split between the two doublet components resulting in a predicted abundance of 0.0000605 for each component. Thus the doublet components should each be (0.0000605/(0.011 - n × 0.000121)) × 100 = 0.55% of the intensity of the unenriched central singlet for each coupling interaction. (n is the number of next neighbor carbons and it must have values between 1 and 4. Therefore, 0.011 - n × 0.000121 is always ≈ 0.011.).
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