Positional information within the MU transposase tetramer: Catalytic contributions of individual monomers

Cell

Volumn 85, Issue 3, 1996, Pages 447-455

  Yang, Jin Ying ^a   Jayaram, Makkuni ^a   Harshey, Rasika M ^a  

^a UNIVERSITY OF TEXAS AT AUSTIN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIOPHAGE DNA; TETRAMER; TRANSPOSASE;

ARTICLE; BACTERIOPHAGE MU; CATALYSIS; DNA CLEAVAGE; DNA TRANSFER; DNA TRANSPOSITION; ENZYME STRUCTURE; NONHUMAN; PRIORITY JOURNAL;

EID: 0029943157     PISSN: 00928674     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0092-8674(00)81122-8     Document Type: Article

References (40)

1. Aldaz, H., Schuster, E., and Baker, T.A. (1996). The interwoven architecture of the Mu transposase couples DNA synapsis to catalysis. Cell 85, in press.
2. Baker, T.A., and Luo, L. (1994). Identification of residues in the Mu transposase essential for catalysis. Proc. Natl. Acad. Sci. USA 91, 6654-6658.
3. Baker, T.A., and Mizuuchi, K. (1992). DNA-promoted assembly of the active tetramer of the Mu transposase. Genes Dev. 6, 2221-2232.
4. Baker, T.A., Mizuuchi, M., and Mizuuchi, K. (1991). Mu B protein allosterically activates strand transfer by the transposase of phage Mu. Cell 65, 1003-1013.
5. Beese, L.S., and Steitz, T.A. (1991). Structural basis for the 3′-5′ exonuclease activity of E. coli DNA polymerase I: a two metal ion mechanism. EMBO J. 10, 25-33.
6. Betermier, M., Alazard, R., Lefrere, V., and Chandler, M. (1989). Functional domains of bacteriophage Mu transposase: properties of C-terminal deletions. Mol. Microbiol. 3, 1159-1171.
7. Carver, T.E., Jr., Hochstrasser, R.A., and Millar, D.P. (1994). Proof-reading DNA: recognition of aberrant DNA termini by the Klenow fragment of DNA polymerase I. Proc. Natl. Acad. Sci. USA 91, 10670-10674.
8. Chen, J.-W., Lee, J., and Jayaram, M. (1992). DNA cleavage in trans by the active site tyrosine during Flp recombination: switching protein partners before exchanging strands. Cell 69, 647-658.
9. Craigie, R., and Mizuuchi, K. (1987). Transposition of Mu DNA: joining of Mu to target DNA can be uncoupled from cleavage at the ends of Mu. Cell 51, 493-501.
10. Craigie, R., Mizuuchi, M., and Mizuuchi, K. (1984). Site-specific recognition of the bacteriophage Mu ends by the Mu A protein. Cell 39, 387-394.
11. Harshey, R.M., and Cuneo, S. (1986). Carboxyl-terminal mutants of phage Mu transposase. J. Genet. 65, 159-174.
12. Jayaram, M. (1994). Phosphoryl transfer in Flp recombination: a template for strand transfer mechanisms. Trends Biochem. Sci. 19, 78-82.
13. Kim, E.E., and Wyckoff, H.W. (1991). Reaction mechanism of alkaline phosphatase based on crystal structures. J. Mol. Biol. 218, 449-464.
14. Kim, K., and Harshey, R.M. (1995). Mutational analysis of the att DNA-binding domain of phage Mu transposase. Nucl. Acids Res. 23, 3937-3943.
15. Kim, K., Namgoong, S.-Y., Jayaram, M., and Harshey, R.M. (1995). Step-arrest mutants of phage Mu transposase: implications in DNA protein assembly, Mu end cleavage, and strand transfer. J. Biol. Chem. 270, 1472-1479.
16. Kuo, C.-F., Zou, A., Jayaram, M., Getzoff, E., and Harshey, R.M. (1991). DNA-protein complexes during attachment-site synapsis in Mu DNA transposition. EMBO J. 10, 1585-1591.
17. Lavoie, B.D., and Chaconas, G. (1995). Transposition of phage Mu DNA. Curr. Topics Microbiol. Immunol. 204, 83-102.
18. Lavoie, B.D., Chan, B.S., Allison, R.G., and Chaconas, G. (1991). Structural aspects of a higher order nucleoprotein complex: induction of an altered DNA structure at the Mu-host junction of the Mu type I transpososome. EMBO J. 10, 3051-3059.
19. Lee, J., and Jayaram, M. (1993). Mechanism of site-specific recombination: logic of assembling recombinase catalytic site from fractional active sites. J. Biol. Chem. 268, 17564-17570.
20. Lee, J., Whang, I., Lee, J., and Jayaram, M. (1994). Directed protein replacement in recombination full sites reveals trans-horizontal DNA cleavage by Flp recombinase. EMBO J. 13, 5346-5354.
21. Lee, J., Whang, I., and Jayaram, M. (1996). Assembly and orientation of Flp recombinase active sites on two-, three- and four-armed DNA substrates. J. Mol. Biol., in press.
22. Leung, P.C., and Harshey, R.M. (1991). Two mutations of phage Mu transposase that affect strand transfer or interactions with B protein lie in distinct polypeptide domains. J. Mol. Biol. 279, 189-199.
23. Leung, P.C., Teplow, D.B., and Harshey, R.M. (1989). Interaction of distinct domains in Mu transposase with Mu DNA ends and an internal transpositional enhancer. Nature 338, 656-658.
24. Mizuuchi, K. (1992). Transpositional recombination: mechanistic insights from studies of Mu and other elements. Annu. Rev. Biochem. 61, 1011-1051.
25. Mizuuchi, M., and Mizuuchi, K. (1989). Efficient Mu transposition requires interaction of transposase with a DNA sequence at the Mu operator: implications for regulation. Cell 58, 399-408.
26. Mizuuchi, M., Baker, T.A., and Mizuuchi, K. (1991). DNase protection analysis of the stable synaptic complexes involved in Mu transposition. Proc. Natl. Acad. Sci. USA 88, 9031-9035.
27. Nakayama, C., Teplow, D.B., and Harshey, R.M. (1987). Structural domains in phage Mu transposase: identification of the site-specific DNA-binding domain. Proc. Natl. Acad. Sci. USA 84, 1809-1813.
28. Namgoong, S.-Y., Jayaram, M., Kim, K., and Harshey, R.M. (1994). DNA-protein cooperativity in the assembly and stabilization of Mu strand transfer complex: relevance of DNA phasing and att site cleavage. J. Mol. Biol. 238, 514-527.
29. Sadowski, P. (1993). Site-specific genetic recombination: hops, flips, and flops. FASEB J. 7, 760-767.
30. Sadowski, P. (1995). The FIp recombinase of the 2-micron plasmid of Saccharomyces cerevisiae. Prog. Nucl. Acids Res. Mol. Biol. 51, 53-91.
31. Savilahti, H., and Mizuuchi, K. (1996). Mu transpositional recombination: donor DNA cleavage and strand transfer in trans by the Mu transposase. Cell 85, in press.
32. Savilahti, H., Rice, P.A., and Mizuuchi, M. (1995). The phage Mu transpososome core: DNA requirements for assembly and function. EMBO J. 14, 4893-4903.
33. Serre, M.C., Evans, B.R., Araki, H., Oshima, Y., and Jayaram, M. (1992). Half-site recombinations mediated by yeast site-specific recombinases Flp and R. J. Mol. Biol. 225, 621-642.
34. Steitz, T.A., Smerdon, S., Jager, J., and Joyce, C.M. (1994). A unified polymerase mechanism for nonhomologous DNAand RNA polymerases. Science 266, 2022-2025.
35. Whang, I., Lee, J., and Jayaram, M. (1994). Active-site assembly and mode of DNA cleavage by FIp recombinase during full-site recombination. Mol. Cell. Biol. 14, 7492-7498.
36. Wu, Z., and Chaconas, G. (1994). Characterization of a region in phage Mu transposase that is involved in interaction with the Mu B protein. J. Biol. Chem. 269, 28829-28833.
37. Wu, Z., and Chaconas, G. (1995). A novel DNA binding and nuclease activity in domain III of Mu transposase: evidence for a catalytic region involved in donor cleavage. EMBO J. 14, 3835-3843.
38. Yang, J.-Y., Jayaram, M., and Harshey, R.M. (1995a). Enhancer independent variants of phage Mu transposase: enhancer-specific stimulation of catalytic activity by a partner transposase. Genes Dev. 9, 2545-2555.
39. Yang, J.-Y., Kim, K., Jayaram, M., and Harshey, R.M. (1995b). A domain sharing model for active site assembly within the Mu A tetramer during transposition: the enhancer may specify domain contributions. EMBO. J. 14, 2374-2384.
40. Zou, A., Leung, P.C., and Harshey, R.M. (1991). Transposase contacts with Mu DNA ends. J. Biol. Chem. 266, 20476-20482.