Squamous carcinoma cell lines fail to respond to 1,25-dihydroxyvitamin D despite normal levels of the vitamin D receptor

Journal of Investigative Dermatology

Volumn 106, Issue 3, 1996, Pages 522-525

  Ratnam, Anita V ^a   Bikle, Daniel D ^a,b   Su, Mei Jhy ^a   Pillai, Sreekumar ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b VETERANS AFFAIRS MEDICAL CENTER   (United States)

Author keywords

Differentiation; Involucrin; Keratinocytes; Transglutaminase

Indexed keywords

CALCITRIOL; INVOLUCRIN; PROTEIN GLUTAMINE GAMMA GLUTAMYLTRANSFERASE; VITAMIN D RECEPTOR;

ARTICLE; CELL DIFFERENTIATION; CONTROLLED STUDY; HUMAN; HUMAN CELL; KERATINOCYTE; PRIORITY JOURNAL; SQUAMOUS CELL CARCINOMA;

EID: 0029942588     PISSN: 0022202X     EISSN: None     Source Type: Journal    
DOI: 10.1111/1523-1747.ep12343898     Document Type: Article

References (13)

1. Boyce ST, Ham RG: Calcium-regulated differentiation of normal human epidermal keratinocytes in chemically defined clonal culture and serum-free serial culture. J Invest Dermatol 81:33s-40s, 1983
2. Bernard BA, Reano A, Darmon YM, Thivolet J: Precocious appearance of involucrin and epidermal transglutaminase during differentiation of psoriatic skin. Br J Dermatol 114:279-283, 1986
3. Bikle DD, Pillai S, Gee E: Squamons carcinoma cell lines produce 1,25 dihydroxyvitamin D, but fail to respond to its prodifferentiating effect. J Invest Dermatol 97:435-441, 1991
4. Bikle DD, Pillai S: Vitamin D, calcium, and epidermal differentiation. Endocr Rev 13:765-784, 1992
5. Chomczynski P, Sacchi N: Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroforrn extraction. Anal Biochem 162:156-159, 1987
6. Hennings H, Michael D, Cheng C, Steinert P, Holbrook K, Yuspa SH: Calcium regulation of growth and differentiation of mouse epidermal cells in culture. Cell 19:245-254, 1980
7. Haussler MR: Vitamin D receptors: nature and function. Annu Rev Nutr 6:527-562, 1986
8. 3, in the differentiation and development of skin. J Invest Dermatol 84:459-460, 1985
9. Milstone LM: Calcium modulates the growth of human keratinocytes. Epithelia 1:129-140, 1987
10. Pillai S, Bikle DD, Elias PM: 1,25-dihydroxyvitamin D production and receptor binding in human keratinocytes varies with differentiation. J Biol Chem 263:5390-5395, 1988a
11. Pillai S, Bikle DD, Hincenbergs M, Elias PM: Biochemical and morphological characterization of growth and differentiation of normal human neonatal keratinocytes in a serum-free medium. J Cell Physiol 134:229-237, 1988b
12. Pillai S, Bikle DD: The role of intracellular free calcium in the cornified envelope formation of keratinocytes: differences in the mode of action of extracellular calcium and 1,25-dihydroxyvitamin D. J Cell Physiol 143:294-302, 1991
13. Su M-J, Bikle DD, Mancianti ML, Pillai S: 1,25 dihydroxyvitamin D potentiates the keratinocyte response to calcium. J Biol Chem 269:14723-14729, 1994