Topographical imaging of macroporous microcarriers using laser scanning confocal microscopy

Journal of Fermentation and Bioengineering

Volumn 81, Issue 5, 1996, Pages 437-444

  Bancel, Stephane ^a   Hu, Wei Shou ^a  

^a University of Minnesota   (United States)

Author keywords

bioreactor; cell culture; confocal microscopy; macroporous microcarriers

Indexed keywords

FLUORESCEIN ISOTHIOCYANATE;

ANCHORAGE INDEPENDENT GROWTH; ARTICLE; BIOREACTOR; CELL ADHESION; CELL CULTURE; CELL DAMAGE; CELL GROWTH; CONFOCAL LASER MICROSCOPY; IMAGE PROCESSING;

MAMMALIA;

EID: 0029941405     PISSN: 0922338X     EISSN: None     Source Type: Journal    
DOI: 10.1016/0922-338X(96)85145-2     Document Type: Article

References (16)

1. Papoutsakis, E.: Fluid mechanical damage of animal cells in bioreactors. Trends Biotechnol., 9, 427-437 (1991).
2. Nilsson, K., Buzsaky, F., and Mosbach, K.: Growth of anchorage-dependent cells on macroporous microcarriers. Bio/ Technology, 4, 989-990 (1986).
3. Looby, D. and Griffiths, B.: Immobilization of animal cells in porous carrier culture. Trends Biotechnol., 8, 204-208 (1990).
4. Reiter, M., Hohenwarter, O., Gaida, T., Zach, N., Schmatz, C., Cluml, G., Weigan, F., Nilsson, K., and Katinger, H.: The use of macroporous gelatin carriers for the cultivation of mammalian cells in fluidized bed reactors. Cytotechnology, 3, 271-277 (1990).
5. Lim, H. S., Choi, J. H., and Kim, J.-H.: Development of macroporous gelatin microcarrier and its characterization for anchorage dependent animal cell culture. Proc. APBioChEC, Kyunglu, Korea, April 22-26, 161-164 (1990).
6. Dean, R. C., Jr., Karkare, S. B., Phillips, P. G., Ray, N. G., and Runstadler, P. W., Jr.: Continuous cell culture with fluidized sponge beds, p. 145-167. In Lyderson, B. K. (ed.), Large scale mammalian cell culture technology. Hanser Publications, New York, NY (1987).
7. Shiragami, N., Ohira, Y., and Unno, H.: Anchorage-dependent animal cell growth in porous microcarrier culture, p. 121-126. In Sasaki, R. and Ikura, K. (ed.), Animal cell culture and production of biologicals. Proceedings of the Third Annual Meeting of the Japanese Association for Animal Cell Technology, Kyoto, Japan, 11-13 December 1990. Kluwer Acad. Pub., Dordrecht, Netherlands (1991).
8. Lee, D. W., Piret, J. M., Gregory, D., Haddow, D. J., and Kilburn, D. G.: Polystyrene macroporous bead support for mammalian cell culture, p. 137-145. In Dibiasio, D. and Mutharason, R. (ed.), Biochemical engineering VII, vol. 665. Ann New York Acad Sci., New York (1992).
9. Reiter, M., Blüml, G., Caida, T., Zach, N., Unicluggauer, F., Doblhoff-Dier, O., Noe, M., Plail, R., Huss, S., and Katinger, H.: Modular integrated fluidized bed bioreactor technology. Bio/Technology, 9, 1110-1102 (1991).
10. Nikolai, T. J. and Hu, W.-S.: Cultivation of mammalian cells on macroporous microcarriers. Enz. Microbial Technol., 14, 203-208 (1992).
11. Lim, H., Han, B., Kim, J., Peshwa, M., and Ha, W.-S.: Spatial distribution of mammalian cells grown on macroporous microcarriers with improved attachment kinetics. Biotechnol. Prog., 8, 486-193 (1992).
12. Himes, V. B. and Hu, W.-S.: Attachment and growth of mammalian cells on microcarriers with different ion exchange capacities. Biotechnol. Bioeng., 29, 1155-1163 (1987)
13. Himes, V. B. and Hu, W.-S.: Attachment and growth of mammalian cells on microcarriers with different ion exchange capacities. Biotechnol. Bioeng., 29, 1155-1163 (1987).
14. Tao, T.-Y., Ji, G.-Y., and Hu, W.-S.: Human fibroblastic cells attach to controlled-charge and gelatin-coated microcarriers at different rates. J. Biotechnol., 6, 9-12 (1987).
15. Levine, D. W., Wang, D. I. C., and Thilly, W. G.: Optimization of growth surface parameters in microcarriers cell culture. Biotechnol. Bioeng., 21, 821-845 (1979).
16. Runstadler, P. W., Ozturk, S. S., and Ray, N. G.: An evaluation of hybridoma cell-specific productivity: perfused immobilized, continuous suspension, and batch suspension culture, p. 57-63. In Murakami, H., Shirahata, S., and Tachibana, H. (ed.), Animal cell technology: basic and applied aspects. Kluwer Acad. Pub., Dordrecht, The Netherlands (1992).