A method for staining of cell nuclei in Xenopus laevis embryos with cyanine dyes for whole-mount confocal laser scanning microscopy

Journal of Histochemistry and Cytochemistry

Volumn 44, Issue 4, 1996, Pages 399-402

  De Mazière, Ann M ^a   Hage, Willem J ^a   Ubbels, Geertje A ^a  

^a HUBRECHT INSTITUTE   (Netherlands)

Author keywords

BOBO 3; Confocal laser scanning microscopy; Cyanine dyes; DNA stain; Egg cleavage; Mitosis; Multicolor fluorescence microscopy; TO PRO 3; Xenopus laevis

Indexed keywords

CYANINE DYE;

ANIMAL TISSUE; ARTICLE; BLASTULA; CELL DIVISION; CELL NUCLEUS; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; EMBRYO; FLUORESCENCE MICROSCOPY; NONHUMAN; PRIORITY JOURNAL; XENOPUS LAEVIS;

EID: 0029940849     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1177/44.4.8601700     Document Type: Article

References (13)

1. Haugland RP. Handbook of fluorescent probes and research chemicals 5th ed. Eugene, OR: Molecular Probes, 1992
2. Rye HS, Yue S, Wemmer DE, Quesada MA, Haugland RP, Mathies RA, Glazer AN. Stable fluorescent complexes of double-stranded DNA with bis-intercalating asymmetric cyanine dyes: properties and applications. Nucleic Acids Res 1992;20:2803
3. Becker B, Clapper J, Harkins KR, Olson JA. In situ screening assay for cell viability using a dimeric cyanine nucleic acid stain. Anal Biochem 1994;221:78
4. Hirons GT, Fawcett JJ, Crissman HA. TOTO and YOYO: new very bright fluorochromes for DNA content analyses by flow cytometry Cytometry 1994;15:129
5. Van Hooijdonk CAEM, Glade CP, Van Erp PEJ. TO-PRO-3 iodide: a novel HeNe laser-excitable DNA stain as an alternative for propidium iodide in multiparameter flow cytometry. Cytometry 1994;17:185
6. Auzanneau I, Barreau C, Salomé L. Imaging by fluorescence video-microscopy of individual single stranded DNA molecules in solution. C R Acad Sci III-Vie 1993;316.459
7. Lee LG, Chen C-H, Chiu LA. Thiazole orange: a new dye for reticulocyte analysis. Cytometry 1986;7:508
8. Kirschner M, Gerhart JC, Hara K, Ubbels GA. Initiation of the cell cycle and establishment of bilateral symmetry in Xenopus eggs In Subtelny S, Wessels NK, eds. The cell surface: mediator of developmental processes New York: Academic Press, 1980:187
9. Harland RM. In situ hybridization, an improved whole-mount method for Xenopus embryos. In Kay BK, Peng HB, eds. Xenopus laevis: practical uses in cell and molecular biology. Methods Cell Biol 1991;36:685
10. Romeis B. Mikroskopische technik. 16th ed. Munchen, Wien R Oldenbourg Verlag, 1968:59
11. Klymkowsky MW, Hanken J Whole-mount staining of Xenopus and other vertebrates. In Kay BK, Peng HB, eds. Xenopus laevis. practical uses in cell and molecular biology. Methods Cell Biol 1991,36 419
12. Czolowska R. Observations on the origin of the "germinal cytoplasm" in Xenopus laevis J Embryol Exp Morphol 1969;22:229
13. Jurand A, Dixon KE. The fine structure of germ plasm in eight-cell embryos developing from normal and inverted eggs of Xenopus laevis. J Exp Zool 1986;237:73