Method to identify biases in PCR amplification of T-cell receptor variable region genes

BioTechniques

Volumn 20, Issue 4, 1996, Pages 600-602

  Daniel, Enoch Satyaselan ^a   Haegert, David George ^a,b  

^a MEMORIAL UNIVERSITY OF NEWFOUNDLAND   (Canada)

^b MEMORIAL UNIVERSITY OF NEWFOUNDLAND   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; DIAGNOSTIC ACCURACY; GENE FREQUENCY; GENE SEQUENCE; GENETIC VARIABILITY; POLYMERASE CHAIN REACTION; T LYMPHOCYTE RECEPTOR GENE;

DNA PRIMERS; GENE AMPLIFICATION; IMMUNOGLOBULIN VARIABLE REGION; PHYTOHEMAGGLUTININS; POLYMERASE CHAIN REACTION; RECEPTORS, ANTIGEN, T-CELL; T-LYMPHOCYTES;

EID: 0029939984     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (10)

1. Choi, Y., B. Kotzin, L. Herron, J. Callahan, P. Marrack and J. Kappler. 1989. Interaction of Staphylococcus aureus toxin "super-antigens" with human T cells. Proc. Natl. Acad. Sci. USA 86:8941-8945.
2. Chomczynski, P. and N. Sacchi. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:156-159.
3. Davey, M.P., M.M. Meyer and A.C. Bakke. 1994. T cell receptor Vβ gene expression in monozygotic twins: discordance in CD8 subset and in disease states. J. Immunol. 152:315-321.
4. Genevée, C., A. Diu, J. Nicrat, A. Caignard, P.-Y. Dietrich, L. Ferradini, S. Roman-Roman, F. Triebel and T. Hercend. 1992. An experimentally validated panel of subfamily-specific oligonucleotide primers (Vα1-w29/ Vβ1-w24) for the study of human T cell receptor variable V gene segment usage by polymerase chain reaction. Eur. J. Immunol. 22:1261-1269.
5. Gulwani-Akolkar, B., B. Shi, P.N. Akolikar, K. Ito, W.B. Bias and J. Silver. 1995 Do HLA genes play a prominent role in determining T cell receptor Vα segment usage in humans? J. Immunol. 154:3843-3851.
6. Lovebridge, J.A., W.M.C. Rosenberg, T.B.L. Kirkwood and J.I. Bell. 1991. The genetic contribution to human T-cell receptor repertoire. Immunology 74:246-250.
7. Munson, J.L., E. van Twuyver, R.J.D. Mooijart, E. Roux, I.J.M. ten Berge and L.P. de Waal. 1995. Missing T-cell receptor Vβ families following blood transfusion. The role of HLA in development of immunization and tolerance. Hum. Immunol. 42:43-53.
8. Panzara, M.A., J.R. Oksenberg and L. Steinman. 1992. The polymerase chain reaction for detection of T-cell antigen receptor expression. Curr. Opm. Immunol. 4:205-210.
9. Wang, A.M. and D.F. Mark. 1990. Quantitative PCR, p. 70-75. In M.A. Innis, D.H. Gelfand, J J. Sninsky and T.J. White (Eds.), PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego.
10. Wucherpfennig, K.W., K. Ota, N. Endo, J.G. Seidman, A. Rosenzweig, H.L. Weiner and D.A. Hafler. 1990. Shared human T cell receptor Vβ usage to immunodominant regions of myelin basic protein. Science 248:1016-1019.