CD-tagging: A new approach to gene and protein discovery and analysis

BioTechniques

Volumn 20, Issue 5, 1996, Pages 896-904

  Jarvik, J W ^a,b   Adler, S A ^a   Telmer, C A ^a   Subramaniam, V ^a   Lopez, A J ^a  

^a CARNEGIE MELLON UNIVERSITY   (United States)

^b Vyrex Corporation   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CHLAMYDOMONAS; CONTROLLED STUDY; DNA SEQUENCE; DROSOPHILA; GENETIC ANALYSIS; GENETIC ENGINEERING; MOLECULAR CLONING; NONHUMAN; PROTEIN ANALYSIS; RNA SPLICING; SEQUENCE TAGGED SITE;

ALGAE; CHLAMYDOMONAS; CHLAMYDOMONAS REINHARDTII; DROSOPHILA MELANOGASTER; MELANOGASTER; METAZOA;

EID: 0029939608     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96205rr03     Document Type: Article

References (31)

1. Bomze, H.M. and A.J. López. 1994. Evolutionary conservation of the structure and expression of alternatively spliced Ultrabithorax isoforms from Drosophila. Genetics 136:965-977.
2. Brand, A. and N. Perrimon. 1993. Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development 118:401-415.
3. Buckler, A.J., D.D. Chang, S.L. Graw, J.D. Brook, D.A. Haber, P.A. Sharp and D.E. Houseman. 1991. Exon amplification: a strategy to isolate mammalian genes based on RNA splicing. Proc. Nat. Acad. Sci. USA 88:4005-4009.
4. Burns, N., B. Grimwade, P.B. Ross-Macdonald, E-Y. Choi, K. Finberg, G.S. Roeder and M. Snyder. 1994. Large-scale analysis of gene expression, protein localization and gene disruption in Saccharomyces cerevisiae. Genes Dev. 8:1087-1105.
5. Crick, F.H.C. 1958. On protein synthesis. Symp. See. Exp. Biol. 12:138-167.
6. Diener, D.R., L.H. Ang and J.L. Rosenbaum. 1993. Assembly of radial spoke proteins in Chlamydomonas: identification of the axoneme binding domain of radial spoke protein 3. J. Cell Biol. 123:183-190.
7. Di Nocera, P.P., and I.B. Dawid. 1983. Transient expression of genes introduced into cultured cells of Drosophila. Proc. Natl. Acad. Sci. USA 80:7095-7098.
8. Dunahay, T.G. 1993. Transformation of Chlamydomonas reinhardtii with silicon carbide whiskers. BioTechniques 15:452-460.
9. Duyk, G.M., S. Kim, R.M. Myers and D.R. Cox. 1990. Exon trapping: a genetic screen to identify candidate transcribed sequences in cloned mammalian genomic DNA. Proc. Nat. Acad. Sci. USA 87:8995-8999.
10. Field, J., J-I. Nikawa, D. Broek, B. MacDonald, L. Rodgers, I.A. Wilson, R.A. Lerner and M. Wigler. 1988. Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method. Mol. Cell. Biol. 8:2159-2165.
11. Friedrich, G. and P. Soriano. 1991. Promoter traps in embryonic stem cells: a genetic screen to identify and mutate developmental genes in mice. Genes Dev. 5:1513-1523.
12. Gavis, E.R. and D.S. Hogness. 1991. Phosphorylation, expression and function of the Ultrabithorax protein family in Drosophila melanogaster. Development 172:1077-1093.
13. Gloor, S., O. Pongs and G. Schmalzing. 1995. A vector for the synthesis of cRNAs encoding Myc epitope-tagged proteins in Xenopus laevis oocytes. Gene 160.213-217.
14. Gossler, A., A.L. Joyner, J. Rossant and W.C. Skarnes. 1989. Mouse embryonic stem cells and reporter constructs to detect developmentally regulated genes. Science 244:463-465.
15. Harris, E.H. 1989. The Chlamydomonas Sourcebook. Academic Press, San Diego, CA.
16. Kolodziej, P. and R.A. Young. 1991. Epitope tagging and protein surveillance. Methods Enzymol. 194:508-594.
17. Kornfeld, K., R.B. Saint, P.A. Beachy, P.J. Harte, D.A. Peattie and D. Hogness. 1989. Structure and expression of a family of Ultrabithorax mRNAs generated by alternative splicing and polyadenylation in Drosophila. Genes Dev. 3:243-258.
18. Krasnow, M.A, E.E. Saffman, K. Kornfeld and D.S. Hogness. 1989. Transcriptional activation and repression by Ultrabithorax protein in cultured Drosophila cells. Cell 60:597-610.
19. López, A.J. and D.S. Hogness. 1991. Immunochemical dissection of the Ultrabithorax homeoprotein family in Drosophila melanogaster. Proc. Natl. Acad. Sci. USA. 88:9924-9928.
20. O'Connor, M.B., R. Binari, L.A. Perkin and W. Bender. 1988. Alternative products from the Ultrabithorax domain of the bithorax complex. EMBO J. 7:435-445.
21. + channel. (GIRK1). J. Biol. Chem. 270:14604-14610.
22. Rabouille, C., N. Hui, F. Hunte, R. Kieckbusch, E.G. Berger, G. Warren and T. Nilsson. 1995. Mapping the distribution of Golgi enzymes involved in the construction of complex oligosaccharides. J. Cell Sci. 108:1617-1627.
23. Rubin, G.M. and A.C. Spradling. 1982. Genetic transformation of Drosophila with transposable element vectors. Science 218:348-353.
24. Senapatby, P., M.B. Shapiro and N.L. Harris. 1990. Computer analysis of protein and nucleic acid sequences. Methods Enzymol. 183:252-278.
25. Sharp, P.A. 1981. Speculations on RNA splicing. Cell 23:643-646.
26. Silflow, C.D. and J. Youngblom. 1986. Chlamydomonas reinhardtii tubulin gene structure. Ann. N.Y. Acad. Sci. 466:18-30.
27. Subramaniam, V. 1995. Ph.D. Thesis. Carnegie Mellon University.
28. Taillon, B.E., S.A. Adler, J.P. Suhan and J.W. Jarvik. 1992. Mutational analysis of centrin: an EF-hand protein associated with three distinct contractile fibers in the basal body apparatus of Chlamydomonas. J. Cell Biol. 119:1613-1624.
29. Williams, B.D., M.A. Velleca, A.M. Curry and J.L. Rosenbaum. 1989. Molecular cloning and sequence analysis of the Chlamydomonas gene coding for radial spoke protein 3: mutation pf-14 is an ochre allele. J. Cell Biol. 109:235-245.
30. Witman, G.B. 1986. Isolation of Chlamydomonas flagella and flagellar axonemes. Methods Enzymol. 134:280-290.
31. Wright, R.L., J.G. Spanier, S.A. Adler and J.W. Jarvik. 1989. The nucleus-basal body connector in Chlamydomonas: evidence for a role in basal body segregation and against essential roles in mitosis or in determining cell polarity. Cell Motil. Cytoskeleton 14:516-526.