Phosphorylation of triton X-100 soluble and insoluble protein substrates in a demembranated/reactivated human sperm model

Molecular Reproduction and Development

Volumn 44, Issue 2, 1996, Pages 200-211

  Leclerc, Pierre ^a   Gagnon, Claude ^a  

^a ROYAL VICTORIA HOSPITAL   (Canada)

Author keywords

Hydrogen peroxide; Protein kinases; Rotenone; Sperm motility

Indexed keywords

ADENOSINE TRIPHOSPHATE; CYCLIC AMP DEPENDENT PROTEIN KINASE; HYDROGEN PEROXIDE; PHOSPHOPROTEIN; TRITON X 100; XANTHINE; XANTHINE OXIDASE;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; HUMAN; HUMAN CELL; MOLECULAR DYNAMICS; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; SPERMATOZOON MOTILITY; SPERMATOZOON TAIL;

CYCLIC AMP-DEPENDENT PROTEIN KINASES; DETERGENTS; HUMANS; MALE; OCTOXYNOL; PHOSPHORYLATION; PROTEINS; SPERMATOZOA; SUBSTRATE SPECIFICITY;

EID: 0029939478     PISSN: 1040452X     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1098-2795(199606)44:2<200::AID-MRD9>3.0.CO;2-R     Document Type: Article

References (52)

1. Acott TS, Carr DW (1984): Inhibition of bovine spermatozoa by caudal epididymal fluid: II. Interaction of pH and a quiescience factor. Biol Reprod 30:926-935.
2. Ashizawa K, Katayama S, Tsuzaki Y (1992): Regulation of flagellar motility by temperature-dependent phosphorylation of a 43 kDa axonemal protein in fowl spermatozoa. Biochem Biophys Res Commun 185:740-745.
3. Babcock DF, Rufo GA Jr, Lardy HA (1983): Potassium-dependent increases in cytosolic pH stimulate metabolism and motility of mammalian sperm. Proc Natl Acad Sci USA 80:1327-1331.
4. Bedford JM (1975): Maturation, transport and fate of spermatozoa in the epididymis. In Hamilton DW, Greep RO (eds): "Handbook of Physiology. Sect. 7, vol. 5." Bethesda: American Physiological Society, pp. 303-317.
5. Brandt H, Hoskins DD (1980): A cAMP-dependent phosphorylated motility protein in bovine epididymal sperm. J Biol Chem 255:982-987.
6. Carr DW, Acott TS (1989): Intracellular pH regulates bovine sperm motility and protein phosphorylation. Biol Reprod 41:907-920.
7. Carrera A, Gerton GL, Moss SB (1994): The major fibrous sheath polypeptide of mouse sperm: Structural and functional similarities to the A-kinase anchoring proteins. Dev Biol 165:272-284.
8. De Jonge CJ, Han HL, Mack SR, Zaneveld LJD (1991): Effect of phorbol diesters, synthetic diacylglycerols, and a protein kinase C inhibitor on the human sperm acrosome reaction. J Androl 12:62-70.
9. de Lamirande E, Gagnon C (1991): Quantitative assessment of the serum induced stimulation of human sperm motility. Int J Androl 13:379-386.
10. de Lamirande E, Gagnon C (1992b): Reactive oxygen species and human spermatozoa 1. Effects on the motility of intact spermatozoa and on sperm axonemes. J Androl 13:368-378.
11. Edelman AM, Blumenthal DK, Krebs EG (1987): Protein serine/threonine kinases. Ann Rev Biochem 56:567-613.
12. Garbers DL, First NL, Lardy HA (1973): Properties of adenosine 3′ 5′-monophosphate-dependent protein kinases isolated from bovine epididymal spermatozoa. J Biol Chem 248:875-879.
13. Garbers DL, Kopf GS (1980): The regulation of spermatozoa by calcium and cyclic nucleotides. Adv Cyclic Nucleotide Res 13:251-307.
14. Garbers DL, Lust WD, First NL, Lardy HA (1971): Effects of phosphodiesterase inhibitors and cyclic nucleotides on sperm respiration and motility. Biochemistry 10:1825-1831.
15. Garty NB, Salomon Y (1987): Stimulation of partially purified adenylate cyclase from bull sperm by bicarbonate. FEBS Lett 218:148-152.
16. Hamasaki T, Barkalow K, Richmond J, Satir P (1991): cAMP-stimulated phosphorylation of an axonemal polypeptide and copurifies with the 22S dynein arm regulates microtubule translocation velocity and swimming speed in Paramecium. Proc Natl Acad Sci USA 88:7918-7922.
17. Hayashi H, Yamamoto K, Yonekawa H, Morisawa M (1987): Involvement of tyrosine protein kinase in the initiation of flagellar movement in rainbow trout spermatozoa. J Biol Chem 262:16692-16698.
18. Horowitz JA, Toeg H, Orr GA (1984): Characterization and localization of cAMP-dependent protein kinases in rat caudal epididymal sperm. J Biol Chem 259:832-838.
19. Horowitz JA, Wasco W, Leiser M, Orr GA (1988): Interaction of the regulatory subunit of a type II cAMP-dependent protein kinase with mammalian sperm flagellum. J Biol Chem 263:2098-2104.
20. Hoskins DD, Casillas ER, Stephens DT (1972): Cyclic AMP-dependent protein kinases of bovine epididymal spermatozoa. Biochem Biophys Res Commun 48:1331-1338.
21. Hoskins DD, Stephens DT, Hall ML (1974): Cyclic adenosine 3′,5′-monophosphate and protein kinase levels in developing bovine spermatozoa. J Reprod Fert 37:131-133.
22. 33Pi into human spermatozoa membrane components. Biol Reprod 17:89-96.
23. Khatoon S, Haley BE, Atherton RW (1985): A comparative analysis of cAMP-dependent protein kinase regulatory subunits in sea urchin and rat spermatozoa. J Androl 6:251-260.
24. Laemmli UK (1970): Cleavage of structural proteins during the assembly of the head of T4. Nature 227:680-685.
25. Lieberman SJ, Wasco W, MacLeod J, Satir P, Orr GA (1988): Immunogold localization of the regulatory subunit of a type II cAMP-dependent protein kinase tightly associated with mammalian sperm flagella. J Cell Biol 107:1809-1816.
26. MacLeod J, Mei X, Erlichman J, Orr GA (1994): Association of the regulatory subunit of a type II cAMP-dependent protein kinase and its binding proteins with the fibrous sheath of rat sperm flagellum. Eur J Biochem 225:107-114.
27. Mohri H, Yanagimachi R (1980): Characteristics of a motor apparatus in testicular, epididymal, and ejaculated spermatozoa. A study using demembranated sperm models. Exp Cell Res 127:191-196.
28. Ndubuka C, Li Y, Rubin CS (1993): Expression of A Kinase anchor protein 75 depletes type II cAMP-dependent protein kinases from the cytoplasm and sequesters the kinases in a particulate pool. J Biol Chem 268:7621-7624.
29. Noland TD, Abumrad NA, Beth AH, Garbers DL (1987): Protein phosphorylation in intact bovine epididymal spermatozoa: Identification of the type II regulatory subunit of cyclic adenosine 3′,5′-monophosphate-dependent protein kinase as an endogenous phosphoprotein. Biol Reprod 37:171-180.
30. Okamura N, Tajima Y, Soejima A, Masuda H, Sugit Y (1985): Sodium bicarbonate in seminal plasma stimulates the motility of mammalian spermatozoa through direct activation of adenylate cyclase. J Biol Chem 260:9699-9705.
31. Pariset C, Feinberg J, Dacheux JL, Øyen O, Jahnsen T, Weinman S (1989): Differential expression and subcellular localization for subunits of cAMP-dependent protein kinase during ram spermatogenesis. J Cell Biol 109:1195-1205.
32. Pariset C, Weinman J, Escaig F, Guyot M, Iftode F, Weinman S, Demaille J (1984): Analytical subcellular distribution of cAMP-dependent protein kinase activity in bull sperm. Gamete Res 10:433-444.
33. Pariset C, Weinman S (1994): Differential localization of two isoforms of the regulatory subunit RIIα of cAMP-dependent protein kinase in human sperm: Biochemical and cytochemical study. Mol Reprod Dev 39:415-422.
34. Paupard MC, MacLeod J, Wasco W, Orr GA (1988): Major 56,000-dalton, soluble phosphoprotein present in bovine sperm is the regulatory subunit of a type II cAMP-dependent protein kinase. J Cell Biochem 37:161-175.
35. Roldan ERS, Harrison RAP (1988): Absence of active protein kinase C in ram spermatozoa. Biochem Biophys Res Commun 155:901-906.
36. 2+-independent induction of acrosome reaction by protein kinase C in human sperm. Endocrinology 131:2235-2243.
37. Rotem R, Paz GF, Homonnai ZT, Kalina M, Naor Z (1990a): Protein kinase C is present in human sperm: possible role in flagellar motility. Proc Natl Acad Sci USA 87:7305-7308.
38. Rotem R, Paz GF, Homonnai ZT, Kalina M, Naor Z (1990b): Further studies on the involvement of protein kinase C in human sperm flagellar motility. Endocrinology 127:2571-2577.
39. San Agustin JT, Witman GB (1994): Role of cAMP in the reactivation of demembranated ram spermatozoa. Cell Motil Cytoskeleton 27:206-218.
40. Schoff PK, Forrester IT, Haley BE, Atherton RW (1982): A study of cAMP binding proteins on intact and disrupted sperm cells using 8-Azidoadenosine 3′,5′-cyclic monophosphate. J Cell Biochem 19:1-15.
41. Smith GD, Vijayaraghavan S, Trautman KC, da Cruz e Silva EF, Wolf DP (1995): Primate sperm contain protein phosphatase 1 (PP1) a biochemical mediator of motility. J Androl (Jan/Feb suppl.) Abst 62.
42. Tash JS, Hidaka H, Means AR (1986): Axokinin phosphorylation by cAMP-dependent protein kinase is sufficient for activation of sperm flagellar motility. J Cell Biol 103:649-655.
43. Tash JS, Kakar SS, Means AR (1984): Flagellar motility requires the cAMP-dependent phosphorylation of a heat-stable NP-40-soluble 56 kd protein, Axokinin. Cell 38:551-559.
44. Tash JS, Means AR (1982): Regulation of protein phosphorylation and motility of sperm by cyclic adenosine monophosphate and calcium. Biol Reprod 26:745-763.
45. Tash JS, Means AR (1983): Cyclic adenosine 3′, 5′ monophosphate, calcium and protein phosphorylation in flagellar motility. Biol Reprod 28:75-104.
46. Tash JS (1990): Role of cAMP, calcium, and protein phosphorylation in sperm motility. In C Gagnon (ed): "Controls of Sperm Motility: Biological and Clinical Aspects." Boca Raton: CRC Press Inc, pp. 229-240.
47. Taylor SS, Buechler JA, Yonemoto W (1990): cAMP-dependent protein kinase: framework for a diverse family of regulatory enzymes. Annu Rev Biochem 59:971-1005.
48. Towbin H, Staehlin T, Gordon J (1979): Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc Natl Acad Sci USA 76:4350-4354.
49. Vijayaraghavan S, Trautman KC, da Cruz e Silva EF (1994): A protein phosphatase (PP1) is involved in the initiation and regulation of sperm motility. Biol Reprod 50 (suppl. 1):150 abst.
50. Visconti PE, Muschietti JP, Flawia MM, Tezon JG (1990): Bicarbonate dependence of cAMP accumulation induced by phorbol esters in hamster spermatozoa. Biochim Biophys Acta 1044:231-236.
51. Visconti PE, Tezon JG (1989): Phorbol esters stimulate cyclic adenosine 3′,5′-mono-phosphate accumulation in hamster spermatozoa during in vitro capacitation. Biol Reprod 40:223-231.
52. Yokota E, Mabuchi I (1990): The cAMP-dependent protein kinase in sea urchin sperm tails: association of the enzyme with the flagellar axonemes. J Biochem 108:1-3.