Suppressor mutations in α-subunit of RNA polymerase for a mutant of the positive regulator, OmpR, in Escherichia coli

FEMS Microbiology Letters

Volumn 139, Issue 2-3, 1996, Pages 175-180

  Kato, Naoki ^a   Aiba, Hirofumi ^a   Mizuno, Takeshi ^a  

^a NAGOYA UNIVERSITY   (Japan)

Author keywords

gene activation; OmpR of Escherichia coli; positive control mutant; RNA polymerase

Indexed keywords

CYCLIC AMP; REGULATOR PROTEIN; RNA POLYMERASE;

ARTICLE; BACTERIUM MUTANT; CONTROLLED STUDY; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN STRUCTURE; TRANSCRIPTION REGULATION;

EID: 0029938842     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-1097(96)00138-3     Document Type: Article

References (17)

1. Mizuno, T. and Mizushima, S. (1990) Signal transduction and gene regulation through the phosphorylation of two regulatory components: the molecular basis for the osmotic regulation of the porin genes. Mol. Microbiol. 4, 1077-1082.
2. Igo, M.M., Slauch, J.M. and Silhavy, T.J. (1990) Signal transduction in bacteria: kinases that control gene expression. New Biol. 2, 5-9.
3. Mizuno, T., Kato, M., Jo, Y.-L. and Mizushima, S. (1988) Interaction of OmpR, a positive regulator, with the osmoregulated ompC and ompF genes of Escherichia coli. J. Biol. Chem. 263, 1008-1012.
4. Aiba, H., Kato, N., Tsuzuki, M. and Mizuno, T. (1994) Mechanism of gene activation by the Escherichia coli positive regulator, OmpR: a mutant defective in transcriptional activation. FEBS Lett. 351, 303-307.
5. Tsuzuki, M., Aiba, H. and Mizuno T (1994) Gene activation of the Escherichia coli positive regulator, OmpR: Phosphorylation-independent mechanism of activation by an OmpR mutant. J. Mol. Biol. 242, 607-613.
6. Kato, N., Tsuzuki, M., Aiba, H. and Mizuno, T. (1995) Gene activation by the Escherichia coli positive regulator OmpR: a mutational study of the DNA-binding domain of OmpR. Mol. Gen. Genet. 248, 399-406.
7. Pratt, L.A. and Silhavy, T.J. (1994) OmpR mutants specifically defective for transcriptional activation. J. Mol. Biol. 243, 579-594.
8. Kato, M., Aiba, H., Tate, S., Nishimura, Y. and Mizuno, T. (1989) Location of phosphorylation site and DNA-binding site of a positive regulator, OmpR, involved in activation of the osmoregulatory genes of Escherichia coli. FEBS Lett. 249, 168-172.
9. Igarashi, K., Hanamura, A., Makino, K., Aiba, H., Aiba, H., Mizuno, T., Nakata, A. and Ishihama, A. (1991) Functional map of the a subunit of Escherichia coli RNA polymerase: two modes of transcription activation by positive factors. Proc. Natl. Acad. Sci. USA 88, 8958-8962.
10. Slauch, J.M., Russo, F.D. and Silhavy, T.J. (1991) Suppressor mutations in rpoA suggest that OmpR controls transcription by direct interaction with the a subunit of RNA polymerase. J. Bacteriol. 173, 7501-7510.
11. Sharif, T.R. and Igo, M.M. (1993) Mutations in the alpha subunit of RNA polymerase that affect the regulation of porin gene transcription in Escherichia coli K-12. J. Bacteriol. 175, 5460-5468.
12. Zou, C., Fujita, N., Igarashi, K. and Ishihama, A. (1992) Mapping the cAMP receptor protein contact site on the α subunit of Escherichia coli RNA polymerase. Mol. Microbiol. 6, 2599-2605.
13. Miller, J., H. (1972) Experiments in Molecular Genetics, pp. 201-205. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
14. Mizuno, T. and Mizushima, S. (1987) Isolation and characterization of deletion mutants of the ompR and envZ, regulatory genes for expression of the outer membrane proteins, OmpC and OmpF, in Escherichia coli. J. Biochem. (Tokyo) 101, 387-396.
15. Jeon, Y.-H., Negishi, T., Shirakawa, M., Yamazaki, T., Fujita, N., Ishihama, A. and Kyogoku, Y. (1995) Solution structure of the activator contact domain of the RNA polymerase α subunit. Science 270, 1495-1497.
16. Tang, H., Severinov, K., Goldfarb, A., Fenyo, D., Chait, B. and Ebright, R.H. (1994) Location, structure, and function of the target of a transcriptional activator protein. Genes Dev. 8, 3058-3067.
17. Tao, K., Zou, C., Fujita, N. and Ishihama, A. (1995) Mapping of the OxyR protein contact site in the C-terminal region of RNA polymerase α subunit. J. Bacteriol. 177, 6740-6744.