A quasi-monoclonal mouse

Science

Volumn 272, Issue 5268, 1996, Pages 1649-1652

  Cascalho, Marilia ^a   Ma, Audrey ^a   Lee, Steven ^a   Masat, Linda ^a   Wabl, Matthias ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

HAPTEN; IMMUNOGLOBULIN HEAVY CHAIN; IMMUNOGLOBULIN LIGHT CHAIN; IMMUNOGLOBULIN M; LYMPHOCYTE ANTIGEN RECEPTOR; MONOCLONAL ANTIBODY;

ANIMAL CELL; ANIMAL EXPERIMENT; ANTIBODY PRODUCTION; ANTIBODY SPECIFICITY; ARTICLE; B LYMPHOCYTE; CONTROLLED STUDY; GENE LOCUS; GENE MUTATION; GENE REARRANGEMENT; GENE TARGETING; HEMIZYGOSITY; IMMUNOGLOBULIN BLOOD LEVEL; IMMUNOGLOBULIN GENE; MOUSE; NONHUMAN; PRIORITY JOURNAL;

EID: 0029937038     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.272.5268.1649     Document Type: Article

References (42)

1. T. Imanishi and O. Mäkelä, J. Exp. Med. 140, 1498 (1974); K. Karjalainen, Eur. J. Immunol. 10, 132 (1980); M. E. White-Scharf and T. Imanishi-Kari, ibid. 11, 897 (1981); ibid. 12, 935 (1982).
2. T. Imanishi and O. Mäkelä, J. Exp. Med. 140, 1498 (1974); K. Karjalainen, Eur. J. Immunol. 10, 132 (1980); M. E. White-Scharf and T. Imanishi-Kari, ibid. 11, 897 (1981); ibid. 12, 935 (1982).
3. T. Imanishi and O. Mäkelä, J. Exp. Med. 140, 1498 (1974); K. Karjalainen, Eur. J. Immunol. 10, 132 (1980); M. E. White-Scharf and T. Imanishi-Kari, ibid. 11, 897 (1981); ibid. 12, 935 (1982).
4. T. Imanishi and O. Mäkelä, J. Exp. Med. 140, 1498 (1974); K. Karjalainen, Eur. J. Immunol. 10, 132 (1980); M. E. White-Scharf and T. Imanishi-Kari, ibid. 11, 897 (1981); ibid. 12, 935 (1982).
5. D. Y. Loh, A. L. M. Bothwell, M. E. White-Scharf, T. Imanishi-Kari, D. Baltimore, Cell 33, 85 (1983).
6. R. Grosschedl, D. Weaver, D. Baltimore, F. Constantini, ibid. 38, 647 (1984).
7. S. Taki, M. Meiering, K. Rajewsky, Science 262, 1268 (1993).
8. J. Chen et al., EMBO J. 12, 821 (1993).
9. J. Chen et al., Int. Immunol. 5, 647 (1993).
10. D. A. Ramsden and G. E. Wu, Proc. Natl. Acad. Sci. U.S.A. 88, 10721 (1991).
11. a and FITC-coupled anti-λ1 and anti-λ2. In a C57BL/6 control mouse, the λ1/λ2 ratio was 76/24. Because the binding site of the monoclonal anti-λ1 is not known, λ3 is detected together with either λ1 or λ2.
12. E. Appella, Proc. Natl. Acad. Sci. U.S.A. 68, 590 (1971); E. P. Schulenberg, E. S. Simms, R. G. Lynch, R. A. Bradshaw, H. N. Eisen, ibid., p. 2623; T. Azuma, L. A. Steiner, H. N. Eisen, ibid. 78, 569 (1981); B. Blomberg, A. Traunecker, H. N. Eisen, S. Tonegawa, ibid., p. 3765; J. Miller, A. Bothwell, U. Storb, ibid., p. 3829; E. Selsing, J. Miller, R. Wilson, U. Storb, ibid. 79, 4681 (1982); B. W. Elliot, H. N. Eisen, L. A. Steiner, Nature 299, 559 (1982); S. Carson and G. E. Wu, Immunogenetics 29, 173 (1989); E. B. Reilly, B. Blomberg, T. Imanishi-Kari, S. Tonegawa, H. N. Eisen, Proc. Natl. Acad. Sci. U.S.A. 81, 2484 (1984).
13. E. Appella, Proc. Natl. Acad. Sci. U.S.A. 68, 590 (1971); E. P. Schulenberg, E. S. Simms, R. G. Lynch, R. A. Bradshaw, H. N. Eisen, ibid., p. 2623; T. Azuma, L. A. Steiner, H. N. Eisen, ibid. 78, 569 (1981); B. Blomberg, A. Traunecker, H. N. Eisen, S. Tonegawa, ibid., p. 3765; J. Miller, A. Bothwell, U. Storb, ibid., p. 3829; E. Selsing, J. Miller, R. Wilson, U. Storb, ibid. 79, 4681 (1982); B. W. Elliot, H. N. Eisen, L. A. Steiner, Nature 299, 559 (1982); S. Carson and G. E. Wu, Immunogenetics 29, 173 (1989); E. B. Reilly, B. Blomberg, T. Imanishi-Kari, S. Tonegawa, H. N. Eisen, Proc. Natl. Acad. Sci. U.S.A. 81, 2484 (1984).
14. E. Appella, Proc. Natl. Acad. Sci. U.S.A. 68, 590 (1971); E. P. Schulenberg, E. S. Simms, R. G. Lynch, R. A. Bradshaw, H. N. Eisen, ibid., p. 2623; T. Azuma, L. A. Steiner, H. N. Eisen, ibid. 78, 569 (1981); B. Blomberg, A. Traunecker, H. N. Eisen, S. Tonegawa, ibid., p. 3765; J. Miller, A. Bothwell, U. Storb, ibid., p. 3829; E. Selsing, J. Miller, R. Wilson, U. Storb, ibid. 79, 4681 (1982); B. W. Elliot, H. N. Eisen, L. A. Steiner, Nature 299, 559 (1982); S. Carson and G. E. Wu, Immunogenetics 29, 173 (1989); E. B. Reilly, B. Blomberg, T. Imanishi-Kari, S. Tonegawa, H. N. Eisen, Proc. Natl. Acad. Sci. U.S.A. 81, 2484 (1984).
15. E. Appella, Proc. Natl. Acad. Sci. U.S.A. 68, 590 (1971); E. P. Schulenberg, E. S. Simms, R. G. Lynch, R. A. Bradshaw, H. N. Eisen, ibid., p. 2623; T. Azuma, L. A. Steiner, H. N. Eisen, ibid. 78, 569 (1981); B. Blomberg, A. Traunecker, H. N. Eisen, S. Tonegawa, ibid., p. 3765; J. Miller, A. Bothwell, U. Storb, ibid., p. 3829; E. Selsing, J. Miller, R. Wilson, U. Storb, ibid. 79, 4681 (1982); B. W. Elliot, H. N. Eisen, L. A. Steiner, Nature 299, 559 (1982); S. Carson and G. E. Wu, Immunogenetics 29, 173 (1989); E. B. Reilly, B. Blomberg, T. Imanishi-Kari, S. Tonegawa, H. N. Eisen, Proc. Natl. Acad. Sci. U.S.A. 81, 2484 (1984).
16. E. Appella, Proc. Natl. Acad. Sci. U.S.A. 68, 590 (1971); E. P. Schulenberg, E. S. Simms, R. G. Lynch, R. A. Bradshaw, H. N. Eisen, ibid., p. 2623; T. Azuma, L. A. Steiner, H. N. Eisen, ibid. 78, 569 (1981); B. Blomberg, A. Traunecker, H. N. Eisen, S. Tonegawa, ibid., p. 3765; J. Miller, A. Bothwell, U. Storb, ibid., p. 3829; E. Selsing, J. Miller, R. Wilson, U. Storb, ibid. 79, 4681 (1982); B. W. Elliot, H. N. Eisen, L. A. Steiner, Nature 299, 559 (1982); S. Carson and G. E. Wu, Immunogenetics 29, 173 (1989); E. B. Reilly, B. Blomberg, T. Imanishi-Kari, S. Tonegawa, H. N. Eisen, Proc. Natl. Acad. Sci. U.S.A. 81, 2484 (1984).
17. E. Appella, Proc. Natl. Acad. Sci. U.S.A. 68, 590 (1971); E. P. Schulenberg, E. S. Simms, R. G. Lynch, R. A. Bradshaw, H. N. Eisen, ibid., p. 2623; T. Azuma, L. A. Steiner, H. N. Eisen, ibid. 78, 569 (1981); B. Blomberg, A. Traunecker, H. N. Eisen, S. Tonegawa, ibid., p. 3765; J. Miller, A. Bothwell, U. Storb, ibid., p. 3829; E. Selsing, J. Miller, R. Wilson, U. Storb, ibid. 79, 4681 (1982); B. W. Elliot, H. N. Eisen, L. A. Steiner, Nature 299, 559 (1982); S. Carson and G. E. Wu, Immunogenetics 29, 173 (1989); E. B. Reilly, B. Blomberg, T. Imanishi-Kari, S. Tonegawa, H. N. Eisen, Proc. Natl. Acad. Sci. U.S.A. 81, 2484 (1984).
18. E. Appella, Proc. Natl. Acad. Sci. U.S.A. 68, 590 (1971); E. P. Schulenberg, E. S. Simms, R. G. Lynch, R. A. Bradshaw, H. N. Eisen, ibid., p. 2623; T. Azuma, L. A. Steiner, H. N. Eisen, ibid. 78, 569 (1981); B. Blomberg, A. Traunecker, H. N. Eisen, S. Tonegawa, ibid., p. 3765; J. Miller, A. Bothwell, U. Storb, ibid., p. 3829; E. Selsing, J. Miller, R. Wilson, U. Storb, ibid. 79, 4681 (1982); B. W. Elliot, H. N. Eisen, L. A. Steiner, Nature 299, 559 (1982); S. Carson and G. E. Wu, Immunogenetics 29, 173 (1989); E. B. Reilly, B. Blomberg, T. Imanishi-Kari, S. Tonegawa, H. N. Eisen, Proc. Natl. Acad. Sci. U.S.A. 81, 2484 (1984).
19. E. Appella, Proc. Natl. Acad. Sci. U.S.A. 68, 590 (1971); E. P. Schulenberg, E. S. Simms, R. G. Lynch, R. A. Bradshaw, H. N. Eisen, ibid., p. 2623; T. Azuma, L. A. Steiner, H. N. Eisen, ibid. 78, 569 (1981); B. Blomberg, A. Traunecker, H. N. Eisen, S. Tonegawa, ibid., p. 3765; J. Miller, A. Bothwell, U. Storb, ibid., p. 3829; E. Selsing, J. Miller, R. Wilson, U. Storb, ibid. 79, 4681 (1982); B. W. Elliot, H. N. Eisen, L. A. Steiner, Nature 299, 559 (1982); S. Carson and G. E. Wu, Immunogenetics 29, 173 (1989); E. B. Reilly, B. Blomberg, T. Imanishi-Kari, S. Tonegawa, H. N. Eisen, Proc. Natl. Acad. Sci. U.S.A. 81, 2484 (1984).
20. E. Appella, Proc. Natl. Acad. Sci. U.S.A. 68, 590 (1971); E. P. Schulenberg, E. S. Simms, R. G. Lynch, R. A. Bradshaw, H. N. Eisen, ibid., p. 2623; T. Azuma, L. A. Steiner, H. N. Eisen, ibid. 78, 569 (1981); B. Blomberg, A. Traunecker, H. N. Eisen, S. Tonegawa, ibid., p. 3765; J. Miller, A. Bothwell, U. Storb, ibid., p. 3829; E. Selsing, J. Miller, R. Wilson, U. Storb, ibid. 79, 4681 (1982); B. W. Elliot, H. N. Eisen, L. A. Steiner, Nature 299, 559 (1982); S. Carson and G. E. Wu, Immunogenetics 29, 173 (1989); E. B. Reilly, B. Blomberg, T. Imanishi-Kari, S. Tonegawa, H. N. Eisen, Proc. Natl. Acad. Sci. U.S.A. 81, 2484 (1984).
21. S. Weiss, J. Meyer, M. R. Wabl, Eur. J. Immunol. 15, 765 (1985).
22. R. Dildrop, A. Gause, W. Muller, K. Rajewsky, ibid. 17, 731 (1987); P. Sanchez and P. A. Cazenave, J. Exp. Med. 166, 265 (1987).
23. R. Dildrop, A. Gause, W. Muller, K. Rajewsky, ibid. 17, 731 (1987); P. Sanchez and P. A. Cazenave, J. Exp. Med. 166, 265 (1987).
24. Material precipitated by sequential incubation of cells with NP conjugated to BSA and antibodies to BSA contained the same polypeptide chains - μ, λ1, and λ2 - as material precipitated with an antiserum to μ and λ. No such material was precipitated with NP-BSA from cells of a C57BL/6 mouse nor with BSA lacking the hapten NP from cells of QM or C57BL/6 mice. These results confirm our flow cytometric data showing that most B cell receptors in the QM mouse bind antigen.
25. H 17.2.25.
26. R. Kleinfield et al., Nature 322, 843 (1986); M. Reth, P. Gehrmann, E. Petrac, P. Wiese, ibid., p. 840; G. B. Beck-Engeser, H.-M. Jäck, M. R. Wabl, Proc. Natl. Acad. Sci. U.S.A. 84, 1060 (1987); R. W. Kleinfield and M. G. Weigert, J. Immunol. 142, 4475 (1989); L. R. Covey, P. Ferrier, F. W. Alt, Int. Immunol. 2, 579 (1990); S. Usuda et al., EMBO J. 11, 611 (1992); T. Shirasawa et al., J. Exp. Med. 176, 1209 (1992); T. Komori, Y. Minami, N. Sakato, H. Sugiyama, Eur. J. Immunol. 23, 517 (1993); E. J. Steenbergen et al., Blood 82, 581 (1993).
27. R. Kleinfield et al., Nature 322, 843 (1986); M. Reth, P. Gehrmann, E. Petrac, P. Wiese, ibid., p. 840; G. B. Beck-Engeser, H.-M. Jäck, M. R. Wabl, Proc. Natl. Acad. Sci. U.S.A. 84, 1060 (1987); R. W. Kleinfield and M. G. Weigert, J. Immunol. 142, 4475 (1989); L. R. Covey, P. Ferrier, F. W. Alt, Int. Immunol. 2, 579 (1990); S. Usuda et al., EMBO J. 11, 611 (1992); T. Shirasawa et al., J. Exp. Med. 176, 1209 (1992); T. Komori, Y. Minami, N. Sakato, H. Sugiyama, Eur. J. Immunol. 23, 517 (1993); E. J. Steenbergen et al., Blood 82, 581 (1993).
28. R. Kleinfield et al., Nature 322, 843 (1986); M. Reth, P. Gehrmann, E. Petrac, P. Wiese, ibid., p. 840; G. B. Beck-Engeser, H.-M. Jäck, M. R. Wabl, Proc. Natl. Acad. Sci. U.S.A. 84, 1060 (1987); R. W. Kleinfield and M. G. Weigert, J. Immunol. 142, 4475 (1989); L. R. Covey, P. Ferrier, F. W. Alt, Int. Immunol. 2, 579 (1990); S. Usuda et al., EMBO J. 11, 611 (1992); T. Shirasawa et al., J. Exp. Med. 176, 1209 (1992); T. Komori, Y. Minami, N. Sakato, H. Sugiyama, Eur. J. Immunol. 23, 517 (1993); E. J. Steenbergen et al., Blood 82, 581 (1993).
29. R. Kleinfield et al., Nature 322, 843 (1986); M. Reth, P. Gehrmann, E. Petrac, P. Wiese, ibid., p. 840; G. B. Beck-Engeser, H.-M. Jäck, M. R. Wabl, Proc. Natl. Acad. Sci. U.S.A. 84, 1060 (1987); R. W. Kleinfield and M. G. Weigert, J. Immunol. 142, 4475 (1989); L. R. Covey, P. Ferrier, F. W. Alt, Int. Immunol. 2, 579 (1990); S. Usuda et al., EMBO J. 11, 611 (1992); T. Shirasawa et al., J. Exp. Med. 176, 1209 (1992); T. Komori, Y. Minami, N. Sakato, H. Sugiyama, Eur. J. Immunol. 23, 517 (1993); E. J. Steenbergen et al., Blood 82, 581 (1993).
30. R. Kleinfield et al., Nature 322, 843 (1986); M. Reth, P. Gehrmann, E. Petrac, P. Wiese, ibid., p. 840; G. B. Beck-Engeser, H.-M. Jäck, M. R. Wabl, Proc. Natl. Acad. Sci. U.S.A. 84, 1060 (1987); R. W. Kleinfield and M. G. Weigert, J. Immunol. 142, 4475 (1989); L. R. Covey, P. Ferrier, F. W. Alt, Int. Immunol. 2, 579 (1990); S. Usuda et al., EMBO J. 11, 611 (1992); T. Shirasawa et al., J. Exp. Med. 176, 1209 (1992); T. Komori, Y. Minami, N. Sakato, H. Sugiyama, Eur. J. Immunol. 23, 517 (1993); E. J. Steenbergen et al., Blood 82, 581 (1993).
31. R. Kleinfield et al., Nature 322, 843 (1986); M. Reth, P. Gehrmann, E. Petrac, P. Wiese, ibid., p. 840; G. B. Beck-Engeser, H.-M. Jäck, M. R. Wabl, Proc. Natl. Acad. Sci. U.S.A. 84, 1060 (1987); R. W. Kleinfield and M. G. Weigert, J. Immunol. 142, 4475 (1989); L. R. Covey, P. Ferrier, F. W. Alt, Int. Immunol. 2, 579 (1990); S. Usuda et al., EMBO J. 11, 611 (1992); T. Shirasawa et al., J. Exp. Med. 176, 1209 (1992); T. Komori, Y. Minami, N. Sakato, H. Sugiyama, Eur. J. Immunol. 23, 517 (1993); E. J. Steenbergen et al., Blood 82, 581 (1993).
32. R. Kleinfield et al., Nature 322, 843 (1986); M. Reth, P. Gehrmann, E. Petrac, P. Wiese, ibid., p. 840; G. B. Beck-Engeser, H.-M. Jäck, M. R. Wabl, Proc. Natl. Acad. Sci. U.S.A. 84, 1060 (1987); R. W. Kleinfield and M. G. Weigert, J. Immunol. 142, 4475 (1989); L. R. Covey, P. Ferrier, F. W. Alt, Int. Immunol. 2, 579 (1990); S. Usuda et al., EMBO J. 11, 611 (1992); T. Shirasawa et al., J. Exp. Med. 176, 1209 (1992); T. Komori, Y. Minami, N. Sakato, H. Sugiyama, Eur. J. Immunol. 23, 517 (1993); E. J. Steenbergen et al., Blood 82, 581 (1993).
33. R. Kleinfield et al., Nature 322, 843 (1986); M. Reth, P. Gehrmann, E. Petrac, P. Wiese, ibid., p. 840; G. B. Beck-Engeser, H.-M. Jäck, M. R. Wabl, Proc. Natl. Acad. Sci. U.S.A. 84, 1060 (1987); R. W. Kleinfield and M. G. Weigert, J. Immunol. 142, 4475 (1989); L. R. Covey, P. Ferrier, F. W. Alt, Int. Immunol. 2, 579 (1990); S. Usuda et al., EMBO J. 11, 611 (1992); T. Shirasawa et al., J. Exp. Med. 176, 1209 (1992); T. Komori, Y. Minami, N. Sakato, H. Sugiyama, Eur. J. Immunol. 23, 517 (1993); E. J. Steenbergen et al., Blood 82, 581 (1993).
34. R. Kleinfield et al., Nature 322, 843 (1986); M. Reth, P. Gehrmann, E. Petrac, P. Wiese, ibid., p. 840; G. B. Beck-Engeser, H.-M. Jäck, M. R. Wabl, Proc. Natl. Acad. Sci. U.S.A. 84, 1060 (1987); R. W. Kleinfield and M. G. Weigert, J. Immunol. 142, 4475 (1989); L. R. Covey, P. Ferrier, F. W. Alt, Int. Immunol. 2, 579 (1990); S. Usuda et al., EMBO J. 11, 611 (1992); T. Shirasawa et al., J. Exp. Med. 176, 1209 (1992); T. Komori, Y. Minami, N. Sakato, H. Sugiyama, Eur. J. Immunol. 23, 517 (1993); E. J. Steenbergen et al., Blood 82, 581 (1993).
35. C. Chen, Z. Nagy, E. L. Prak, M. Weigert, Immunity 3, 747 (1995).
36. S. L. Tiegs, D. M. Russell, D. Nemazee, J. Exp. Med. 177, 1009 (1993); D. Gay, T. Saunders, S. Camper, M. Weigert, ibid., p. 999.
37. S. L. Tiegs, D. M. Russell, D. Nemazee, J. Exp. Med. 177, 1009 (1993); D. Gay, T. Saunders, S. Camper, M. Weigert, ibid., p. 999.
38. N. K. Jerne, Annal. D. Immunol. 125C, 373 (1974); A. Coutinho, Scand. J. Immunol. 42, 3 (1995); F. Varela et al., Proc. Natl. Acad. Sci. U.S.A. 88, 5917 (1991).
39. N. K. Jerne, Annal. D. Immunol. 125C, 373 (1974); A. Coutinho, Scand. J. Immunol. 42, 3 (1995); F. Varela et al., Proc. Natl. Acad. Sci. U.S.A. 88, 5917 (1991).
40. N. K. Jerne, Annal. D. Immunol. 125C, 373 (1974); A. Coutinho, Scand. J. Immunol. 42, 3 (1995); F. Varela et al., Proc. Natl. Acad. Sci. U.S.A. 88, 5917 (1991).
41. μ. The shorter, 5′ sequence contains DQ52, the most proximal D segment, and flanking sequences. The cloning strategy was as follows: The 5′ homologous region, a 1.6-kb Hind III-Bam HI DNA fragment containing DQ52, was cloned into the same sites of Pmc1Neo (Stratagene). Subsequently, the Xho I site 5′ of the DQ52 segment was destroyed by nucleotide incorporation with the Klenow fragment and blunt-end ligation. The Xho I-Sal I herpes simplex virus-thymidine kinase (HSVtk) gene was then cloned into the remaining Xho I site. The Hind III site 5′ of DQ52 was also eliminated to allow cloning of the heavy chain gene [the Sal I-Xho I fragment from plasmid pμ (3)] into the Hind III site 5′ of the HSVtk gene.
42. H17.2.25 and NP-BSA; D. Huszar and N. Lonberg of GenPharm for providing the heavy chain and κ double knockout mice; R. Murray and P. Vieira for advice on generating replacement mice; and C. Steinberg for editorial assistance. Supported by NIH grants 1R01 GM37699 and P60 AR20684, the Engalitcheff Award of the Arthritis Foundation, the Lucille P. Markey Charitable Trust, the Junta Nacional de Investigação Científica e Tecnológica-Praxis XXI-BD 3763/94 (M.C.), and the American-Italian Foundation for Cancer Research (L.M.).