Resting-cell dehydrogenase assay measuring a novel water-soluble formazan detects catabolic differences among cells

Journal of Microbiological Methods

Volumn 25, Issue 1, 1996, Pages 49-55

  Leverone, M R ^a   Owen, T C ^a   Tieder, F S ^a   Stewart, G J ^b   Lim, D V ^a  

^a UNIVERSITY OF SOUTH FLORIDA   (United States)

^b UNIVERSITY OF WEST GEORGIA   (United States)

Author keywords

carbohydrate metabolism; dehydrogenase; tetrazolium

Indexed keywords

CARBOHYDRATE; FORMAZAN; OXIDOREDUCTASE; TETRAZOLIUM;

ARTICLE; BACILLUS SUBTILIS; CARBOHYDRATE METABOLISM; CATABOLISM; CONTROLLED STUDY; ENZYME ACTIVITY; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PSEUDOMONAS;

EID: 0029936249     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/0167-7012(95)00084-4     Document Type: Article

References (26)

1. Rodriquez, G.G., Phipps, D., Ishiguro, K. and Ridgeway, H.F. (1992) Use of a fluorescent redox probe for direct visualization of actively respiring bacteria. Appl. Environ. Microbiol. 58, 1801-1808.
2. Schaule, G., Flemming, H.-C. and Ridgeway, H.F. (1993) Use of 5-cyano-2,3-ditolyl tetrazolium chloride for quantifying planktonic and sessile respiring bacteria in drinking water. Appl. Environ. Microbiol. 59, 3850-3857.
3. Roslev, P. and King, G.M. (1993) Application of a tetrazolium salt with a water-soluble formazan as an indicator of viability in respiring bacteria. Appl. Environ. Microbiol. 59, 2891-2896.
4. Comley, J.C.W., and Turner, C.H. (1990) Potential of a soluble tetrazolium/formazan assay for the evaluation of filarial viability. Int. J. Parasitol. 20, 251-255.
5. Scudiero, D.A., Shoemaker, R.H., Paull, K.D., Monks, A., Tierny, S., Nofziger, T.H., Currens, M.J., Senif, D. and Boyd, M.R. (1988) Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines. Cancer Res. 48, 4827-4833.
6. Tellier, R., Krajden, M., Grigoriew, G.A. and Campbell, I. (1992) Innovative endpoint determination system for antifungal susceptibility testing of yeasts. Antimicrob. Agents Chemother. 36, 1619-1625.
7. Cesari, I.M., Rieber, M. and Imaeda, T. (1969) Localization and properties of enzymes involved with electron transport activity in mycobacteria. J. Bacteriol. 98, 767-773.
8. Trevors, J.T. 1984. Electron transport system activity in soil, sediment, and pure cultures. CRC Crit. Rev. Microbiol. 11, 83-100.
9. Matson, E.A. and Buck, J.D. (1987) Community electron transport of procaryotes in euryoxic estuarine sediment. Mar. Ecol. Prog. Ser. 41, 71-78.
10. Blenkinsopp, S.A., and Lock, M.A. (1990) The measurement of electron transport system activity in river biofilms. Water Res. 24, 441-445.
11. Gammelgaard, A., Freeman, C. and Lock, M.A. (1992) Measurement of electron transport system activity in sphagnum-derived peat. Soil Biol. Biochem. 25, 505-507.
12. Paull, K.D., Shoemaker, R.H., Boyd, M.R., Parsons, J.L., Risbood, P.A., Barbera, W.A., Sharma, M.N., Baker, D.C., Hand, E., Scudiero, D.A., Monks, A., Alley, M.C. and Grote, M. (1988) The synthesis of XTT: A new tetrazolium reagent that is bioreducible to a water-soluble formazan. J. Heterocycl. Chem. 25, 911-914.
13. Barltrop, J.A. and Owen, T.C. (1991) 5-(3-carboxymethoxyphenyl)-2-(4,5,-dimethylthiazolyl)-3-(4-sulfophenyl) tetrazolium, inner salt (MTS) and related analogs of 3-(4,5-dimehtylthiazolyl)-2,5-diphenyltetrazolium bromide (MTT) reducing to purple water-soluble formazans as cell-viability indicators. Bioorg. Med. Chem. Lett. 1, 611-614.
14. Buttke, T.M., McCubrey, J.A. and Owen, T.C. (1993) Use of an aqueous soluble tetrazolium/formazan assay to measure viability and proliferation of lymphokine-dependent cell lines. J. Immunol. Methods. 157, 233-240.
15. Cory, A.H., Owen, T.C., Barltrop, J.A. and Cory, J.G. (1991) Use of an aqueous soluble tetrazolium/formazan assay for cell growth assays in culture. Cancer Commun. 3, 207-212.
16. Carlson, C.A., Pierson, L.S., Rosen, J.J. and Ingraham, J.L. (1983) Pseudomonas stutzeri and related species undergo natural transformation. J. Bacteriol. 15, 93-99.
17. Klein, K., Steinberg, R., Fiethen, B. and Overath, P. (1971) Fatty acid degradation in Escherichia coli. An inducible system for the uptake of fatty acids and further characterization of old mutants. Eur. J. Biochem. 19, 442-450.
18. Maloy, S.R., Ginsburgh, C.L., Simons, R.W. and Nunn, W.D. (1981) Transport of long and medium chain fatty acids by Escherichia coli. J. Biol. Chem. 256, 3735-3742.
19. Whitaker, J.R. and Granum, P.E. (1980) An absolute method for protein determination based on difference in absorbance at 235 and 280 nm. Anal. Biochem. 109, 156-159.
20. Adhya, S., and Echols, H. (1966) Glucose effect and the galactose enzymes of Escherichia coli: correlation between glucose inhibition of induction and inducer transport. J. Bacteriol. 92, 601-608.
21. Faith, Jr., W.T., Giorgio, Jr., N.A. and Mallette, M.F. (1964) Mechanism of glucose inhibition of β-galactosidase biosynthesis in resting cultures of Escherichia coli. Arch. Biochem. Biophys. 108, 430-439.
22. Weeks, G., Shapiro, M., Burns, R.O. and Wakil, S.J. (1969) Control of fatty acid metabolism. J. Bacteriol. 97, 827-836.
23. Nunn, W.D. (1986) A molecular view of fatty acid catabolism in Escherichia coli. J. Bacteriol. 50, 179-192.
24. Ohne, M. (1975) Regulation of the dicarboxylic acid part of the citric acid cycle in Bacillus subtilis. J. Bacteriol. 122, 224-234.
25. Lessie, T.G. and Phibbs, Jr., P.V. (1984) Alternative pathways of carbohydrate utilization in pseudomonads. Annu. Rev. Microbiol. 38, 359-387.
26. Wolff, J.A., MacGregor, C.H., Eisenberg, R.C. and Phibbs, Jr., P.V. (1991) Isolation and characterization of catabolite repression control mutants of Pseudomonas aeruginosa PAO. J. Bacteriol. 173, 4700-4706.