A new approach using multiplex long accurate PCR and yeast artificial chromosomes for bacterial chromosome mapping and sequencing

Genome Research

Volumn 6, Issue 5, 1996, Pages 448-453

  Sorokin, Alexei ^a   Lapidus, Alla ^a   Capuano, Veronique ^a   Galleron, Nathalie ^a   Pujic, Petar ^a   Ehrlich, S Dusko ^a  

^a CEMAGREF   (France)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL GENETICS; GENE MAPPING; GENE MUTATION; GENE SEQUENCE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; YEAST ARTIFICIAL CHROMOSOME;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS);

EID: 0029935873     PISSN: 10549803     EISSN: None     Source Type: Journal    
DOI: 10.1101/gr.6.5.448     Document Type: Article

References (17)

1. Anagnostopoulos, C., P.J. Piggot, and J.A. Hoch. 1993. The genetic map of Bacillus subtilis 168. In Bacillus subtilis and other gram-positive bacteria (ed. A.L. Sonenshein, J.A. Hoch, and R. Losick), pp. 425-461. American Society for Microbiology, Washington, D.C.
2. Anand, R., A. Villasante, and C. Tyler-Smith. 1989. Construction of yeast artificial chromosome libraries with large inserts using fractionation by pulsed-field electrophoresis. Nucleic Acids Res. 17: 3425-3433.
3. Azevedo, V., E. Alvarez, E. Zumstein, G. Damiani, V. Sgaramella, S.D. Ehrlich, and P. Serror. 1993. An ordered collection of Bacillus subtilis DNA segments cloned in yeast artificial chromosomes. Proc. Natl. Acad. Sci. 90: 6047-6051.
4. Barnes, W.M. 1994. PCR amplification of up to 35-kb DNA with high fidelity and high yield from λ bacteriophage templates. Proc. Natl. Acad. Sci. 91: 2216-2220.
5. Burke, D.T., G.F. Carle, and M.W. Olson. 1987. Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors. Science 236: 806-812.
6. Cheng, S., C. Fockler, W.M. Barnes, and R. Higuchi. 1994. Effective amplification of long targets from cloned inserts and human genomic DNA. Proc. Natl. Acad. Sci. 91: 5695-5699.
7. Cohen, D., I. Chumakov, and J. Weissenbach. 1993. A first-generation physical map of the human genome. Nature 366: 698-701.
8. Dear, S. and R. Staden. 1991. A sequence assembly and editing for efficient management of large projects. Nucleic Acids Res. 19:3907-3911.
9. Dower, W.J., J.F. Miller, and C.W. Ragsdale. 1988. High-efficiency transformation of E. coli by high-voltage electroporation. Nucleic Acids Res. 16: 6127-6145.
10. Errington, J. 1986. A general method for fusion of the Escherichia coli lacZ gene to chromosomal genes in Bacillus subtilis. J. Gen. Microbiol. 132: 2953-2961.
11. Kunst, F., A. Vassaroti, and A. Danchin. 1995. Organization of the European Bacillus subtilis genome sequencing project. Microbiology 141: 249-255.
12. Marshall, E. 1995. A strategy for the sequencing of the genome 5 years early. Science 267: 783-784.
13. Ogasawara, N., Y. Fujita, Y. Kobayashi, Y. Sadaie, T. Tanaka, H. Takahashi, K. Yamane, and H. Yoshikawa. 1995. Systematic sequencing of the Bacillus subtilis genome: Progress report of the Japanese group. Microbiology 141: 257-259.
14. Sambrook, J., E.F. Fritsch, and T. Maniatis. 1989. Molecular cloning: A laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
15. Sorokin, A.V., E. Zumstein, V. Azevedo, S.D. Ehrlich, and P. Serror. 1993. The organisation of the Bacillus subtilis 168 chromosome region between the spoVA and serA genetic loci, based on sequence data. Mol. Microbiol. 10(2): 397-405.
16. Sorokin, A.V., P. Serror, P. Pujic, V. Azevedo, and S.D. Ehrlich. 1995. The Bacillus subtilis chromosome region encoding homologues of the Eschericliia coli mssA and rpsA gene products. Microbiology 141: 311-319.
17. Sorokin, A.V., V. Azevedo, E. Zumstein, N. Galleron, S.D. Ehrlich, and P. Serror. 1996. Sequence analysis of the Bacillus subtilis chromosome region between the serA and kdg loci cloned in a yeast artificial chromosome. Microbiology (in press).