Functional domains of moloney murine leukemia virus integrase defined by mutation complementation analysis

Journal of Virology

Volumn 70, Issue 7, 1996, Pages 4585-4597

  Jonsson, Colleen B ^a,b   Donzella, George A ^a   Gaucan, Elena ^a   Smith, Christine M ^a   Roth, Monica J ^a  

^a RUTGERS ROBERT WOOD JOHNSON MEDICAL SCHOOL   (United States)

^b NEW MEXICO STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

INTEGRASE; VIRUS ENZYME; VIRUS RNA;

ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; DNA STRAND; DNA TRANSFER; ENZYME ACTIVE SITE; ENZYME LOCALIZATION; GENE MUTATION; GENETIC COMPLEMENTATION; MOLONEY LEUKEMIA ONCOVIRUS; NONHUMAN; PRIORITY JOURNAL;

ANIMALS; BASE SEQUENCE; BINDING SITES; CATALYSIS; DNA NUCLEOTIDYLTRANSFERASES; DNA PRIMERS; GENETIC COMPLEMENTATION TEST; INTEGRASES; MOLECULAR SEQUENCE DATA; MOLONEY MURINE LEUKEMIA VIRUS; MUTAGENESIS; SEQUENCE DELETION; VIRUS INTEGRATION;

EID: 0029934077     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/jvi.70.7.4585-4597.1996     Document Type: Article

References (52)

1. Bowerman, B., P. O. Brown, J. M. Bishop, and H. E. Varmus. 1989. A nucleoprotein complex mediates the integration of retroviral DNA. Genes Dev. 3:469-478.
2. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye hinding. Anal. Biochem. 72:248-254.
3. Bushman, F. D., A. Engelman, I. Palmer, P. Wingfield, and K. Craigie. 1993. Domains of the integrase protein of human immunodeficiency virus type I responsible for polynucleolidyl transfer and zinc binding. Proc. Natl. Acad. Sci. USA 90:3428-3432.
4. Bushman, F. D., and B. Wang. 1994. Rous sarcoma virus integrase protein: mapping functions for catalysis and substrate binding. J. Virol. 68:2215-2223.
5. Chow, S. A., and P. O. Brown. 1995. Juxtaposition of two viral DNA ends in a bimolecular disintegration reaction mediated by multimers of human immunodeficiency virus type 1 or murine leukemia virus inlegrase. J. Virol. 68:7869-7878.
6. Chow, S. A., K. A. Vincent, V. Ellison, and P. O. Brown. 1992. Reversal of integration and DNA splicing mediated by integrase of human immunodeficiency virus. Science 255:723-726.
7. Coffin, J. M. 1990. Retroviridae and their replication, p. 645-708. In B. N. Fields and D. M. Knipe (ed.). Fundamental virology. Raven Press, New York.
8. Colicelli, J., and S. P. Gaff. 1985. Mutants and pseudorevertants of Moloney murine leukemia virus with alterations at the integration site. Cell 42:573-580.
9. Craigie, R., T. Fujiwara, and F. Bushman. 1990. The IN protein of Moloney murine leukemia virus processes the viral DNA ends and accomplishes their integration in vitro. Cell 62:829-837.
10. Donehower, L. A. 1988. Analysis of mutant Moloney murine leukemia viruses containing linker insertion mutations in the 3′ region of pol. J. Virol. 62: 3958-3964.
11. Donzella, G. A., C. B. Jonsson, and M. J. Roth. 1993. Influence of substrate structure on disintegration by Moloney murine leukemia virus integrase. J. Virol. 67:71177-7087.
12. Dotan, I., B. P. Scottoline, T. S. Heuer, and P. O. Brown. 1995. Characterization of recombinanl murine leukemia virus integrase. J. Virol. 69:456-468.
13. Ellison, V., J. Gerton, K. A. Vincent, and P. O. Brown. 1995. An essential interaction between distinct domains of HIV-1 integrase mediates assembly of the active multimer. J. Biol. Chem. 270:3320-3326.
14. Engelman, A., F. D. Bushman, and R. Craigie. 1993. Identification of discrete functional domains of HIV-1 integrase and their organization within an active multimeric complex. BMBO J. 12:3269-3275.
15. Engelman, A., and R. Craigie. 1992. Identification of conserved amino acid residues critical for human immunodeficiency virus type 1 integrase function in vitro. J. Virol. 66:6361-6369.
16. Engelman, A., K. Mizuuchi, and R. Craigie. 1991. HIV-1 DNA integration: mechanism of viral DNA cleavage and DNA strand transfer. Cell 67:1211-1221.
17. Farnet, C. M., and W. A. Haselline. 1991. Determination of viral proteins present in the human immunodeficiency virus type 1 preintegration complex. J. Virol. 65:1910-1915.
18. Felkner, R. H., and M. J. Roth. 1992. Mutalional analysis of the N-linked glycosylation sites of the SU protein of Moloney murine leukemia virus. J.Virol. 66:4258-4264.
19. Ho, S. N., H. D. Huut, R. M. Horton, J. K. Pullen, and L. R. Pease. 1989. Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 77:51-59.
20. Johnson, M. S., M. A. McClure, D. F. Feng, J. Gray, and R. F. Doolittle. 1986. Computer analysis of retroviral pol genes: assignment of enzymatic functions to specific sequences and homologies with nonviral enzymes. Proc. Natl. Acad. Sci. USA 83:7648-7652.
21. Jonsson, C. B., G. A. Donzella, and M. J. Roth. 1993. Characterization of the forward and reverse integration reactions of the Moloney murine leukemia virus integrase protein purified from Escherichia coli. J. Biol. Chem. 268: 1462-1469.
22. Jonsson, C. B., and M. J. Roth. 1993. Role of the His-Cys finger of the Moloney murine leukemia virus integrase protein in integration and disintegration. J. Virol. 67:5562-5571.
23. Katz, R. A., and A. M. Skalka. 1994. The retroviral enzymes. Annu. Rev. Biochem. 63:133-173.
24. Katzman, M., R. A. Katz, A. M. Skalka, and J. Leis. 1989. The avian retroviral integration protein cleaves the terminal sequences of linear viral DNA at the in vivo sites of integration. J. Virol. 63:5319-5327.
25. Khan, F., J. P. G. Mack, R. A. Katz, J. Kulkosky, and A. M. Skalka. 1990. Retroviral integrase domains: DNA binding and the recognition of LTR sequences. Nucleic Acids Res. 19:851-860.
26. Kulkosky, J., K. S. Jones, R. A. Katz, J. P. G. Mack, and A. M. Skalka. 1992 Residues critical for retroviral integrative recombination in a region that is highly conserved among retroviral/retrotransposon integrases and bacterial insertion sequence transpnsases. Mol. Cell. Biol. 12:2331-2338.
27. Kunkel, T. A., J. D. Roberts, and R. A. Zakour. 1987. Rapid and efficient site-specific mutagenesis without a phenotypic selection. Methods Enzymol. 154:367.
28. Leavitt, A. D., L. Shiue, and H. E. Varmus. 1993. Site-directed mutagenesis of HIV-1 integrase demonstrates differential effects on intesrase functions in vitro. J. Biol. Chem. 268:2113-2119.
29. Lin, T. H., T. P. Quinn, D. Grandgenett, and M. T. Walsh. 1989. Secondary structural analysis of retrovirus integrase: characterization by circular dichroism and empirical prediction methods. Proteins Struct. Fund. Genet. 5:156-165.
30. Mazumder, A., A. Engelman, R. Craigie, M. Fesen, and Y. Pommier. 1994. Intermodular disintegration and intramolecular strand transfer activities of wild type and mutant HIV-1 integrase. Nucleic Acids Res. 22:1037-1043.
31. Miller, D. G., M. A. Adam, and A. D. Miller. 1990. Gene transfer by retrovirus vectors occurs only in cells that are actively replicating at the time of infection. Mol. Cell. Biol. 10:4239-4242.
32. Mumm, S. R., and D. P. Grandgenett. 1991. Defining nucleic acid-binding properties of avian retrovirus integrase by deletion analysis. J. Virol. 65: 1160-1167.
33. Murphy, J. E., T. de los Santos, and S. P. Goff. 1993. Mutational analysis of the sequences at the termini of the Moloney murine leukemia virus DNA required for integration. Virology 195:432-440.
34. Murphy, J. E., and S. P. Goff. 1992. A mutation at one end of Moloney murine leukemia virus DNA blocks cleavage of both ends by the viral integrase in vivo. J. Virol. 66:5092-5095.
35. Puras-Lutzke, R., C. Vink, and R. H. A. Plasterk. 1994. Characterization of the minimal DNA-binding domain of the HIV integrase protein. Nucleic Acids Res. 22:4125-4131.
36. Roe, T., T. C. Reynolds, G. Yu, and P.O. Brown. 1993. Integration of murine leukemia virus DNA depends on mitosis. EMBO J. 12:2099-2108.
37. Roth, M., P. Schwartzberg, N. Tanese, and S. P. Goff. 1990. Analysis of mutations in the integration function of Moloney murine leukemia virus: effects on DNA binding and cutting. J. Virol. 64:4709-4717.
38. Roth, M. J. 1991. Mutational analysis of the carboxyl terminus of the Moloney murine leukemia virus integration protein. J. Virol. 65:2141-2145.
39. Roth, M. J., P. L. Schwartzberg, and S. P. Goff. 1989. Structure of the termini of DNA intermediates in the integration of retroviral DNA: dependence on IN function and terminal DNA sequence. Cell 58:47-54.
40. Sanger, F., S. Nicklen, and A. R. Coulson. 1978. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
41. Schwartzberg, P. L., M. J. Roth, N. Tanese, and S. P. Goff. 1993. Analysis of a temperature-sensitive mutation affecting the integration protein of Moloney murine leukemia virus. Virology 192:673-678.
42. Shinnick, T. M., R. A. Lerner, and J. G. Sutcliffe. 1981. Nucleotide sequence of Moloney tnurine leukemia virus. Nature (London) 293:543-548.
43. van Den Ent, F. I., C. Vink, and R. H. A. Plasterk. 1995. DNA substrate activities of the human immunodeficiency virus type I integrase protein. J. Virol. 68:7825-7832.
44. van Gent, D. C., Y. Elgersma, M. W. J. Bolk, C. Vink, and R. H. A. Plasterk. 1991. DNA binding properties of the integrase proteins of human immunodeficiency viruses types I and 2. Nucleic Acids Res. 19:3821-3827.
45. van Gent, D. C., A. A. M. O. Groenger, and R. H. A. Plasterk. 1992. Mutational analysis of the integrase protein of human immunodeficiency virus type 2. Proc. Natl. Acad. Sci. USA 89:9598-9602.
46. van Gent, D. C., C. Vink, A. A. M. O. Groeneger, and R. H. A. Plasterk. 1993. Complementation between HIV integrase proteins mutated in different domains. EMBO J. 12:3261-3267.
47. Varmus, H., and P. Brown. 1989. Retroviruses. American Society for Microbiology, Washington, D.C.
48. Vink, C., A. A. M. O. Groenger, and R. H. A. Plasterk. 1993. Identification of the catalytic and DNA-binding region of the human immunodeficiency virus type 1 integrase protein. Nucleic Acids Res. 21:1419-1425.
49. Vink, C., E. Yeheskiely, C. A. van der Marel, J. H. van Boom, and R. H. A. Plasterk. 1993. Site specific hydrolysis and alcohulysis of human immunodeficiency virus DNA termini mediated by the viral integrase protein. Nucleic Acids Res. 19:6691-6658.
50. Vogelstein, B., and D. Gillespie. 1979. Preparative and analytical purification of DNA from agarose. Proc. Natl. Acad. Sci. USA 76:615-619.
51. Woerner, A. M., M. Klulch, J. G. Uvin, and C. J. Marcus-Sekura. 1992. Localization of DNA hinding activity of HIV-1 integrase to the C-terminal half of the protein. AIDS Res. Hum. Retroviruses 8:297-304.
52. Woerner, A. M., and C. J. Marcus-Sekura. 1993. Characterization of a DNA binding domain in the C-terminus of HIV-1 integrase by deletion mutagenesis. Nucleic Acids Res. 21:3507-3511.