Molecular and functional characterization of a mutant allele of the mitogen-activated protein-kinase gene SLT2(MPK1) rescued from yeast autolytic mutants

Current Genetics

Volumn 29, Issue 6, 1996, Pages 516-522

  Martin H ^a   Castellanos, M C ^a   Cenamor, R ^a   Sanchez M ^a   Molina, M ^a   Nombela, C ^a  

^a UNIVERSIDAD COMPLUTENSE DE MADRID   (Spain)

Author keywords

Caffeine; MAP kinase; SLT2; Yeast

Indexed keywords

SACCHAROMYCES CEREVISIAE; FUNGI;

CAFFEINE; FUNGAL PROTEIN; MITOGEN ACTIVATED PROTEIN KINASE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; AUTOLYSIS; CONTROLLED STUDY; ENZYME STRUCTURE; FUNGAL GENETICS; FUNGUS MUTANT; GENE ISOLATION; GENETIC COMPLEMENTATION; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

ALLELES; AMINO ACID SEQUENCE; BASE SEQUENCE; BINDING SITES; CA(2+)-CALMODULIN DEPENDENT PROTEIN KINASE; CAFFEINE; DNA PRIMERS; DNA, FUNGAL; FUNGAL PROTEINS; GENES, FUNGAL; MITOGEN-ACTIVATED PROTEIN KINASES; MOLECULAR SEQUENCE DATA; MUTATION; POINT MUTATION; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

EID: 0029933836     PISSN: 01728083     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02426955     Document Type: Article

References (41)

1. Bossemeyer D (1994) The glycine-rich sequence of protein kinases: a multifunctional element. Trends Biochem Sci 19:201-205
2. 2 + -ATPase-like proteins and their relationships to the agonist-sensitive calcium store in adrenal chromafin cells. Nature 342:72-74
3. Cid VJ, Durán A, del Rey F, Snyder MP, Nombela C, Sánchez M (1995) Molecular basis of cell integrity and morphogenesis in Saccharomyces cerevisiae. Microbiol Rev 59: 345-386
4. Costigan C, Gehrung S, Snyder M (1992) A synthetic lethal screen identifies SLK1, a novel protein kinase homolog implicated in yeast morphogenesis and cell growth. Mol Cell Biol 12: 1162-1178
5. Costigan C, Kolodrubetz D, Snyder M (1994) NHP6A and NHP6B, which encode HMG1-like proteins, function downstream in the yeast SLT2 MAPK pathway. Mol Cell Biol 14:2391-2403
6. De Bondt HL, Rosenblatt J, Jancarik J, Jones HD, Morgan DO, Kim SH (1993) Crystal structure of cyclin-dependent kinase 2. Nature 363:595-602
7. Dever TE, Glynias MJ, Merrick WC (1987) GTP-binding domain: three consensus sequence elements with distinct spacing. Proc Natl Acad Sci USA 84:1814-1818
8. Downes CS, Musk SRR, Watson JV, Johnson RT (1990) Caffeine overcomes a restriction point associated with DNA replication, but does not accelerate mitosis. J Cell Biol 110:1855-1859
9. Fuente JM de la, Alvarcz A, Nombela C, Sanchez M (1992) Flow cytometric analysis of Saccharomyces cerevisiae autolytic mutants and protoplasts. Yeast 8:39-45
10. Gietz RD, Sugino A (1988) New yeast-Escherichia coli shuttle vectors constructed with in vitro-mutagenized yeast genes lacking six-base pair restriction sites. Gene 74:527-534
11. Hanahan D (1985) Techniques for transformation of E. coli. In: Glover DM (ed) DNA Cloning. IRL Press, Oxford, England, pp 120-121
12. Hanks SK, Quinn AM, Hunter T (1988) The protein kinase family: conserved features and deduced phylogeny of the catalytic domains. Science 241:42-52
13. Irie K, Takase M, Lee KS, Levin DE, Araki H, Matsumoto K, Oshima Y (1993) MKK1 and MKK2, which encode Saccharomyces cerevisiae mitogen-activated protein kinase-kinase homologs, function in the pathway mediated by protein kinase C. Mol Cell Biol 13:3076-3083
14. Ito H, Fukuda Y, Murata K, Kimura A (1983) Transformation of intact yeast cells treated with alkali cations. J Bacteriol 153:163-168
15. Jäntti J, Kuismanen E (1993) Effect of caffeine and reduced temperature (20 °C) on the organization of the pre-Golgi and the Golgi stack membranes. J Cell Biol 120:1321-1335
16. Kamada Y, Jung US, Piotrowski J, Levin DE (1995) The protein kinase C-activated MAP kinase pathway of Saccharomyces cerevisiae mediates a novel aspect of the heat-shock response. Genes Dev 9:1559-1571
17. Knighton DR, Zheng JH, Ten Eyck LF, Ashford VA, Xuong NH, Taylor SS, Sowadski JM (1991) Crystal structure of the catalytic subunit of cyclic adenosine monophosphate-dependent protein kinase. Science 253:407-414
18. Lee KS, Levin DE (1992) Dominant mutations in a gene encoding a putative protein kinase (BCK1) bypass the requirement for a Saccharomyces cerevisiae protein kinase C homolog. Mol Cell Biol 12:172-182
19. Lee KS, Hines LK, Levin DE (1993 a) A pair of functionally redundant yeast genes(PPZ1 and PPZ2) encoding type 1-related protein phosphatase function within the PKC1-mediated pathway. Mol Cell Biol 13:5843-5853
20. Lee KS, Irie K, Gotoh Y, Watanabe Y, Araki H, Nishida E, Matsumoto K, Levin DE (1993 b) A yeast MAP kinase homolog (MPK1) mediates signaling by protein kinase C. Mol Cell Biol 13:3067-3075
21. Levin DE, Fields FO, Kunisawa R, Bishop JM, Thorner J (1990) A candidate protein-kinase C gene, PKC1, is required for the S. cerevisiae cell cycle. Cell 62:213-224
22. Martin H, Arroyo J, Sanchez M, Molina M, Nombela C (1993) Activity of the yeast MAP-kinase homologue Slt2 is critically required for cell integrity at 37 °C. Mol Gen Genet 241:177-184
23. Mazzoni C, Zarov P, Rambourg A, Mann C (1993) The SLT2 (MPK1) MAP kinase homolog is involved in polarized cell growth in Saccharomyces cerevisiae. J Cell Biol 123:1821-1833
24. Mellor J, Dobson MJ, Roberts NA, Tuite MF, Emtage JS, White S, Lowe PA, Patel T, Kingsman AJ, Kingsman SM (1983) Efficient synthesis of enzimatically active calf chymosin in Saccharomyces cerevisiae. Gene 24:1-14
25. Odawara M, Kadowaki T, Yamamoto R, Shibasaki Y, Tobe K (1989) Human diabetes associated with a mutation in the tyrosine-kinase domain of the insulin receptor. Science 245:66-68
26. Paravicini G, Cooper M, Friedli L, Smith DJ, Carpentier JL, Klig LS, Payton MA (1992) The osmotic integrity of the yeast cell requires a functional PKC1 gene product. Mol Cell Biol 12:4896-4905
27. Parsons WJ, Ramkumar V, Stiles GL (1988) Isobutyl-methilxanthine stimulates adenylate cyclase by blocking the inhibitory regulatory protein, Gi. Mol Pharmacol 34:37-41
28. Pelley RJ, Maihle NJ, Boerkoel C, Shu HK, Carter TH (1989) Disease tropism of v-erb-B: effects of carboxyl-terminal tyrosine and internal mutations on tissue-specific transformation. Proc Natl Acad Sci USA 86:7164-7168
29. Posas F, Casamayor A, Ariño J (1993) The PPZ protein phosphatases are involved in the manteinance of osmotic stability of yeast cells. FEBS Lett 318:282-286.
30. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
31. Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463-5467
32. Sherman F, Fink GR, Hicks JB (1983) Methods in yeast genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
33. 1 for progression into S phase. Mol Cell Biol 11:2133-2148
34. Taylor SS, Knighton DR, Zheng J, Ten Eyck LF, Sowadski JM (1992) Structural framework for the protein-kinase family. Annu Rev Cell Biol 8:429-462
35. Torres L, Martin H, Garcia-Saez MI, Arroyo J, Molina M, Sanchez M, Nombela C (1991) A protein-kinase gene complements the lytic phenotype of Saccharomyces cerevisiae lyt2 mutants. Mol Microbiol 5:2845-2854
36. Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of protein from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350-4354
37. Uesono Y, Fujita A,Toh-e A, Kikuchi Y (1994) The MCS1/SSD1/ SRK1/SSL1 gene is involved in stable maintenance of the chromosome in yeast. Gene 143:135-138
38. Walker JE, Saraste M, Runswick MJ, Gay NJ (1982) Distantly related sequences in the a- and b-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide-binding fold. EMBO J 1:945-951
39. Wilson RB, Brenner AA, White TB, Engler MJ, Gaughran JP, Tatchell K (1991) The Saccharomyces cerevisiae SRK1 gene, a suppressor of bcy1 and ins1, may be involved in protein-phosphatase function. Mol Cell Biol 11:3369-3373
40. Winston F, Chumley F, Fink GR (1983) Eviction and transplacement of mutant genes in yeast. Methods Enzymol 101:211-228
41. Zhang F, Strand A, Robbins D, Cobb MH, Goldsmith EJ (1994) Atomic structure of the MAP-kinase ERK2 at 2.3 Å resolution. Nature 367:704-711