Rate limitations in posttranslational processing by the mammary gland of transgenic animals

Annals of the New York Academy of Sciences

Volumn 782, Issue , 1996, Pages 87-96

  Subramanian, Anuradha ^a   Paleyanda, Rekha K ^c   Lubon, Henryk ^c   Williams, Barry L ^b   Gwazdauskas, Francis C ^b   Knight, James W ^b   Drohan, William N ^c   Velander, William H ^a  

^a State University   (United States)

^b VIRGINIA POLYTECHNIC INSTITUTE AND STATE UNIVERSITY   (United States)

^c HOLLAND LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM BINDING PROTEIN; GLUTAMIC ACID; PLASMA PROTEIN; PROTEIN C; VITAMIN K GROUP;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ANIMAL EXPERIMENT; BIOREACTOR; CARBOXYLATION; CONFERENCE PAPER; CONTROLLED STUDY; MAMMARY GLAND; MOUSE; NONHUMAN; PARTIAL THROMBOPLASTIN TIME; PROMOTER REGION; PROTEIN CONFORMATION; PROTEIN EXPRESSION; PROTEIN PROCESSING; PROTEIN PURIFICATION; PROTEIN STRUCTURE; SWINE; TRANSGENIC ANIMAL;

ANIMALIA; MURINAE; MUS MUSCULUS; SUS SCROFA;

EID: 0029932985     PISSN: 00778923     EISSN: None     Source Type: Book Series    
DOI: 10.1111/j.1749-6632.1996.tb40550.x     Document Type: Conference Paper

References (20)

1. YAN, S. C. B., P. RAZZANO, Y. B. CHAO, J. D. WALLS, D. T. BERG, D. B. MCCLURE & B. W. GRINNELL. 1990. Characterization and novel purification of recombinant human protein C from three mammalian cell lines. Bio/Technology 8: 655-661.
2. CLARK, A. J., P. SIMONS, I. WILMUT & R. LATHE. 1987. Pharmaceuticals from transgenic livestock. Trends Biotechnol. 5: 20-24.
3. CLARK, A. J., H. BESSOS, J. O. BISHOP, P. BROWN, S. HARRIS, R. LATHE, M. MCCLENAGHAN, C. PROWSE, J. P. SIMONS, C. B. A. WHITELAW & I. WILMUT. 1989. Expression of human anti-hemophilic factor IX in the milk of transgenic sheep. Bio/Technology 7: 492.
4. DENMAN, J., M. HAYES, C. O'DAY, T. EDMUNDS, C. BARTLETT, S. HIRANI, K. M. EBERT, K. GORDON & J. M. MCPHERSON. 1991. Transgenic expression of a variant of human tissue-type plasminogen activator in goat milk: Purification and characterization of the recombinant enzyme. Bio/Technology 9: 839-843.
5. KAUFMAN, R. J., L. C. WASLEY, B. C. FURIE, B. FURIE & C. B. SHOEMAKER. 1986. Expression, purification and characterization of recombinant γ-carboxylated factor IX synthesized in Chinese hamster ovary cells. J. Biol. Chem. 261: 9622-9628.
6. 2-terminal region of protein C. J. Biol. Chem. 264: 18781.
7. VELANDER, W. H., R. L. PAGE, T. MORCOL, C. G. RUSSELL, R. CANSECO, J. M. YOUNG, W. N. DROHAN, F. C. GWAZDAUSKAS, T. D. WILKINS & J. L. JOHNSON. 1992. Production of biologically active human protein C in the milk of transgenic mice. Ann. N.Y. Acad. Sci. 665: 391-103.
8. WALLS, J. D., D. T. BERG, S. B. YAN & B. W. GRINNELL. 1989. Gene 81: 139.
9. WALL, R. J., V. G. PURSEL, A. SHAMAY, R. A. MCKNIGHT, C. W. PITTIUS & L. HENNINGHAUSEN. 1991. High-level synthesis of a heterologous milk protein in the mammary glands of transgenic swine. Proc. Natl. Acad. Sci. USA 88: 1696-1700.
10. BERG, G. T., D. B. MCCLURE, J. D. WALLS, S. B. YAN & B. W. GRINNELL. 1991. Exp. Cell Res. 192: 32.
11. MORCOL, T., R. M. AKERS, J. L. JOHNSON, B. L. WILLIAMS, F. C. GWAZDAUSKAS, J. W. KNIGHT, H. LUBON, R. K. PALEYANDA, W. N. DROHAN & W. H. VELANDER. 1994. The porcine mammary gland as a bioreactor for complex proteins. Ann. N.Y. Acad. Sci. 721: 218.
12. DUBE S., J. W. FISHER & J. S. POWELL. 1988. Glycosylation at specific sites of erythropoietin is essential for biosynthesis, secretion, and biological activity. J. Biol. Chem. 263: 17516-17521.
13. ESMON, C. T. 1987. The regulation of natural anticoagulant pathways. Science 235: 1348-1352.
14. WU, S. M., D. P. MORRIS & D. W. STAFFORD. Identification and purification to near homogeneity of the vitamin K-dependent carboxylase. 1991. Proc. Natl. Acad. Sci. USA 88: 2236-2240.
15. YAN, S. B., B. W. GRINNELL & F. WOLD. 1989. Post-translational modifications of proteins. Trends Biotechnol. 14: 264.
16. MILETICH, J. P. & G. J. BROZE, JR. 1990. β-Protein C is not glycosylated at asparagine 329. J. Biol. Chem. 265: 11397-11404.
17. DROHAN, W. N., D. ZHANG, R. K. PALEYANDA, R. CHANG, M. WROBLE, W. H. VELANDER & H. LUBON. 1994. Inefficient processing of human protein C in the mouse mammary gland. Transgenic Res. 3: 355-364.
18. VELANDER, W. H., J. L. JOHNSON, R. L. PAGE, C. G. RUSSELL, A. SUBRAMANIAN, T. D. WILKINS, F. C. GWAZDAUSKAS, C. PITTIUS & W. N. DROHAN. 1992. High-level expression of a heterologous protein in the milk of transgenic swine using the cDNA encoding human protein C. Proc. Natl. Acad. Sci. USA 89: 12003-12007.
19. GRINELL, B. W., D. T. BERG, J. WALLS & S. B. YAN. 1987. Trans-activated expression of fully γ-carboxylated recombinant human protein C, an antithrombitic factor. Bio/ Technology 5: 1189-1192.
20. FOSTER, D. C., S. YOSHITAKE & E. W. DAVIE. 1985. The nucleotide sequence of the gene for human protein C. Proc. Natl. Acad. Sci. USA 82: 4673-4677.