A recombination-efficient baculovirus vector for simultaneous expression of multiple genes

Gene

Volumn 171, Issue 2, 1996, Pages 209-213

  Chatterji, Udayan ^a   Ahmad, Raies ^a   Venkaiah, B ^a   Hasnain, Seyed E ^a  

^a NATIONAL INSTITUTE OF IMMUNOLOGY   (India)

Author keywords

Firefly luciferase; hsp70 promoter; Human chorionic gonadotropin hormone; lacZ; Multiple expression vector; Polyhedrin promoter; Stoichiometric levels; galactosidase

Indexed keywords

BETA GALACTOSIDASE; CHORIONIC GONADOTROPIN; HEAT SHOCK PROTEIN; LUCIFERASE; VIRUS DNA;

ARTICLE; AUTOGRAPHA CALIFORNICA; BACULOVIRUS; DNA TRANSFECTION; DROSOPHILA MELANOGASTER; EXPRESSION VECTOR; GENE EXPRESSION; MOLECULAR CLONING; NONHUMAN; POLYHEDROSIS VIRUS; PRIORITY JOURNAL; PROMOTER REGION; RECOMBINANT GENE; REPORTER GENE; RESTRICTION SITE; TRANSCRIPTION REGULATION; VIRUS RECOMBINANT;

EID: 0029932933     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-1119(96)00188-6     Document Type: Article

References (23)

1. Belayev, A.S. and Roy, P.: Development of baculovirus triple and quadruple expression vectors: co-expression of three or four BTV proteins and the synthesis of bluetongue virus-like particles in insect cells. Nucleic Acids Res. 21 (1993) 1219-1223.
2. Belayev, A.S., Hails, R.S. and Roy, P.: High-level expression of five foreign genes by a single recombinant baculovirus. Gene 156 (1995) 229-233.
3. Gilmour, D.S. and Lis, J.T.: In vivo interactions of RNA polymerase II with genes of Drosophila melanogaster. Mol. Cell. Biol. 5 (1985) 2009-2018.
4. Hasnain, S.E. and Nakhai, B.: Expression of the gene encoding firefly luciferase in insect cells using a baculovirus vector. Gene 91 (1990) 135-138.
5. Hasnain, S.E., Nakhai, B., Ehtesham, N.Z., Sridhar, P., Ranjan, A., Talwar, G.P. and Jha, P.K.: β-Subunit of human chorionic gonadotropin hormone and firefly luciferase simultaneously synthesized in insect cells using a recombinant baculovirus are differentially expressed and transported. DNA Cell Biol. 13 (1994) 275-282.
6. Kalderon, D., Roberts, B.L., Richardson, W.D. and Smith, A.E.: A short amino acid sequence able to specify nuclear location. Cell 39 (1984) 499-509.
7. Kitts, P.A., Ayres, M.D. and Possee, R.D.: Linearization of baculovirus DNA enhances the recovery of recombinant virus expression vectors. Nucleic Acids Res. 18 (1990) 5667-5672.
8. Kitts, P.A. and Possee, R.D.: A method for producing recombinant baculovirus expression vectors at high frequency. BioTechniques 14 (1993) 810-817.
9. Leusch, M.S., Lee, S.C. and Olins, P.O.: A novel host-vector system for direct selection of recombinant baculoviruses (bacmids) in Escherichia coli. Gene 160 (1995) 191-194.
10. Luckow, V.A., Lee, S.C., Barry, G.F. and Olins, P.O.: Efficient generation of infectious recombinant baculo viruses by site-specific transposon-mediated insertion of foreign genes into a baculovirus genome propagated in Escherichia coli. J. Virol. 67 (1993) 4566-4579.
11. Miller, J.H.: A Short Course In Bacterial Genetics. A Laboratory Manual and Handbook for Escherichia coli and Related Bacteria. CSHL Press, Cold Spring Harbor, NY, 1992.
12. Nakhai, B., Sridhar, P., Pal, R., Talwar, G.P. and Hasnain, S.E.: Overexpression and characterisation of recombinant beta subunit of human chorionic gonadotropin hormone synthesized in insect cells infected with a genetically engineered baculovirus. Ind. J. Biochem. Biophys. 29 (1992) 315-321.
13. O'Reilly, D.R., Miller, L.K. and Luckow, V.A.: Baculovirus Expression Vectors. A Laboratory Manual. W.H. Freeman, New York, NY, 1992.
14. Patel, G., Nasmyth, K. and Jones, N.: A new method for the isolation of recombinant baculovirus. Nucleic Acids Res. 20 (1992) 97-104.
15. Peakman, T.C., Harris, R. and Gewart, D.R.: Highly efficient generation of recombinant baculoviruses by enzymatically mediated site-specific in vitro recombination. Nucleic Acids Res. 20 (1992) 495-500.
16. Pennock, G.D., Shoemaker, C. and Miller, L.K.: Strong and regulated expression of Escherichia coli β-galactosidase in insect cells with a baculovirus vector. Mol. Cell. Biol. 4 (1984) 399-406.
17. Sambrook, J., Fritsch, E.F. and Maniatis, T.: Molecular Cloning. A Laboratory Manual, 2nd ed. CSHL Press, Cold Spring Harbor, NY, 1989.
18. Sridhar, P. and Hasnain, S.E.: Differential secretion and glycosylation of recombinant human chorionic gonadotropin (βhCG) synthesized using different promoters in the baculovirus expression vector system. Gene 131 (1993) 261-264.
19. Velazquez, J.M., Sonoda, S., Bugaisky, G. and Lindquist, S.: Is the major Drosophila heat shock protein present in cells that have not been heat shocked? J. Cell Biol. 96 (1983) 286-290.
20. Wang, X., Ooi, B.G. and Miller, L.K.: Baculovirus vectors for multiple expression and for occluded virus production. Gene 100 (1991) 131-137.
21. deWet, J.R., Wood, K.V., Helinski, D.R. and DeLuca, M.: Cloning of firefly luciferase cDNA and expression of active luciferase in E. coli. Proc. Natl. Acad. Sci. USA 82 (1985) 7870-7873.
22. Weyer, U., Knight, S. and Possee, R.D.: Analysis of very late gene expression by Autographa californica nuclear polyhedrosis virus and the further development of multiple expression vectors. J. Gen. Virol. 71 (1990) 1525-1534.
23. Zuidema, D., Schouten, A., Usmany, M., Maule, A.J., Belsham, G.J., Roosien, J., Klinge-Roode, E.C., Van Lent, J.W.M. and Vlak, J.M.: Expression of cauliflower mosaic virus gene I in insect cells using a novel polyhedrin-based baculovirus expression vector. J. Gen. Virol. 71 (1990) 2201-2209.