Detection and localization of syntrophic propionate-oxidizing bacteria in granular sludge by in situ hybridization using 16S rRNA-based oligonucleotide probes

Applied and Environmental Microbiology

Volumn 62, Issue 5, 1996, Pages 1656-1663

  Harmsen, Hermie J M ^a   Kengen, Harry M P ^a,b   Akkermans, Antoon D L ^a   Stams, Alfons J M ^a   De Vos, Willem M ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

^b RADBOUD UNIVERSITY NIJMEGEN   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

PROPIONIC ACID;

ARTICLE; BACTERIUM COLONY; BACTERIUM DETECTION; ECOSYSTEM; FLUORESCENCE; HYBRIDIZATION; IN SITU HYBRIDIZATION; NONHUMAN; OLIGONUCLEOTIDE PROBE; SLUDGE;

ARCHAEA; BACTERIA (MICROORGANISMS); METHANOSAETA;

EID: 0029932879     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.62.5.1656-1663.1996     Document Type: Article

References (33)

1. Amann, R. I., B. J. Binder, R. J. Olson, S. W. Chisholm, R. Devereux, and D. A. Stahl. 1990. Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. Appl. Environ. Microbiol. 56:1919-1925.
2. Amann, R. I., W. Ludwig, and K. H. Schleifer. 1995. Phylogenetic identification and in situ detection of individual microbial colls without cultivation. Microbiol. Rev. 59:143-169.
3. Boone, D. R., and M. P. Bryant. 1980. Propionate-degrading bacterium, Syntrophobacter wolinii sp. nov. gen. nov., from methanogenic ecosystems. Appl. Environ. Microbiol. 40:626-632.
4. 2 and formate formation during syntrophic butyrate and propionate degradation. Anaerobe 1:35-39.
5. Dörner, C. 1992. Biochemie und Energetik der Wasserstoff-Freisetzung in der syntrophen Vergärung von Fettsäuren und Benzoat. Doctoral thesis. Eberhard-Karls-University, Tübingen, Germany.
6. Grotenhuis, J. T. C., M. Smit, C. M. Plugge, X. Yuansheng, A. A. M. van Lammeren, A. J. M. Stams, and A. J. M. Zehnder. 1991. Bacteriological composition and structure of granular sludge adapted to different substrates. Appl. Environ. Microbiol. 57:1942-1949.
7. Guiot, S. R., A. Pauss, and J. W. Costerton. 1992. A structured model of the anaerobic granule consortium. Water Sci. Technol. 25:1-10.
8. Harmsen, H. J. M., H. M. P. Kengen, A. D. L. Akkermans, and A. J. M. Stams. 1995. Phylogenetic analysis of two syntrophic propionate-oxidizing bacteria in enrichments cultures. Syst. Appl. Microbiol. 18:67-73.
9. Harmsen, H. J. M., B. Wullings, A. D. L. Akkermans, W. Ludwig, and A. J. M. Stams. 1993. Phylogenetic analysis of Syntrophobacter wolinii reveals a relationship with sulfate-reducing bacteria. Arch. Microbiol. 160:238-240.
10. Heppner, B., G. Zellner, and H. Diekmann. 1992. Start-up and operation of a propionate-degrading fluidized-bed reactor. Appl. Microbiol. Biotechnol. 36:810-816.
11. Jetten, M. S. M., A. J. M. Stams, and A. J. B. Zehnder. 1992. Methanogenesis from acetate: a comparison of the acetate metabolism in Methanothrix soehngenii and Methanosarcina spp. FEMS Microbiol. Rev. 88:181-198.
12. Lens, P. N. L., D. de Beer, C. C. H. Cronenberg, F. P. Houwen, S. P. P. Ottengraf, and W. H. Verstraete. 1993. Hetrogeneous distribution of microbial activity in methanogenic aggregates: pH and glucose microprofiles. Appl. Environ. Microbiol. 59:3803-3815.
13. Lettinga, G. 1995. Anaerobic digestion and wastewater treatment systems. Antonie Leeuwenhoek 67:3-28.
14. Macario, A. J. L., F. A. Visser, J. B. van Lier, and E. Conway de Macario. 1991. Topography of methanogenic subpopulations in a microbial consortium adapting to thermophilic conditions. J. Gen. Microbiol. 137:2179-2189.
15. MacLeod, F. A., S. R. Guiot, and J. W. Costerton. 1990. Layered structure of bacterial aggregates produced in an upflow anaerobic sludge bed and filter reactor. Appl. Environ. Microbiol. 56:1598-1607.
16. Maidak, B. L., N. Larsen, M. J. McCaughey, R. Overbeek, G. J. Olsen, K. Fogel, J. Blandy, and C. R. Woese. 1994. The ribosomal database project. Nucleic Acids Res. 22:3485-3487.
17. McInerney, M. J. 1991. The genus Syntrophomonas and other syntrophic anaerobes, p. 2048-2057. In A. Balows, H. G. Trüper, M. Dworkin, W. Harder, and K. H. Schleifer (ed.). The prokaryotes. Springer-Verlag, Berlin.
18. Oude Elferink, S. J. W. H. Personal communication.
19. Oude Elferink, S. J. W. H., R. N. Maas, H. J. M. Harmsen, and A. J. M. Stams. Desulforhabdus amnigenus gen. nov. sp. nov., a sulfate reducer isolated from anaerobic granular sludge. Arch. Microbiol. 164:119-124.
20. Prensier, G., H. C. Dubourguier, I. Thomas, G. Albagnac, and M. N. Buison. 1988. Specific Immunological probes for studying the bacterial associations in granules and biofilms, p. 55-61. In G. Lettinga, A. J. B. Zehnder, J. T. C. Grotenhuis, and L. W. Hulshoff Pol (ed.). Granular anaerobic sludge: microbiology and technology. Pudoc, Wageningen, The Netherlands.
21. Raskin, L., L. K. Poulsen, D. R. Noguera, B. E. Rittmann, and D. A. Stahl. 1994. Quantification of methanogenic groups in anaerobic biological reactors by oligonucleotide probe hybridization. Appl. Environ. Microbiol. 60:1241-1248.
22. Raskin, L., J. M. Stromley, B. E. Rittman, and D. A. Stahl. 1994. Group-specific 16S rRNA hybridization probes to describe natural communities of methanogens. Appl. Environ. Microbiol. 60:1232-1240.
23. Roller, C., M. Wagner, R. Amann, W. Ludwig, and K. H. Schleifer. 1994. In situ probing of Gram-positive bacteria with high DNA G + C content using 23S rRNA-targeted oligonucleotides. Microbiology 140:2849-2858.
24. Rutten, T. L. M., and J. Derksen. 1992. Microtubules in pollen tubes subprotoplasts: organization during protoplast formation and protoplast out-growth. Protoplasma 167:231-237.
25. Stahl, D. A., and R. Amann. 1991. Development and application of nucleic acid probes, p. 205-248. In E. Stackebrandt and E. Goodfellow (ed.), Nucleic acid techniques in bacterial systematics. John Wiley & Sons, Chichester, United Kingdom.
26. Stahl, D. A., B. Flesher, H. W. Mansfield, and L. Montgomery. 1988. Use of phylogenetically based hybridization probes for studies of ruminal microbial ecology. Appl. Environ. Microbiol. 54:1079-1084.
27. Stams, A. J. M. 1994. Metabolic interactions between anaerobic bacteria in methanogenic environments. Antonic Leeuwenhock 66:271-294.
28. Stams, A. J. M., J. B. Van Dijk, C. Dijkema, and C. Plugge. 1993. Growth of syntrophic propionate-oxidizing bacteria with fumarate in the absence of methanogenic bacteria. Appl. Environ. Microbiol. 59:1114-1119.
29. Visser, A., I. Beeksma, F. van der Zee, A. J. M. Stams, and G. Lettinga. 1993. Anaerobic degradation of volatile fatty acids at different sulphate concentrations. Appl. Microbiol. Biotechnol. 40:549-556.
30. Visser, F. A., J. B. van Lier, A. J. L. Macario, and E. Conway de Macario. 1991. Diversity and population dynamics of methanogenic bacteria in a granular consortium. Appl. Environ. Microbiol. 57:1728-1734.
31. Wagner, M., R. Amann, P. Kämpfer, B. Assmus, A. Hartman, P. Hutzier, N. Springer, and K. H. Schleifer. 1994. Identification and in situ detection of Gram-negative filamentous bacteria in activated sludge. Syst. Appl. Microbiol. 17:405-417.
32. Wu, W. M., R. F. Hickey, and J. G. Zeikus. 1991. Characterization of metabolic performance of methanogenic granules treating brewery wastewater: role of sulfate-reducing bacteria. Appl. Environ. Microbiol. 57:3438-3449.
33. Wu, W. M., K. J. Mahendra, E. Conway de Macario, J. H. Thiele, and J. G. Zeikus. 1992. Microbial composition and characterization of prevalent methanogens and acetogens isolated from syntrophic methanogenic granules. Appl. Microbiol. Biotechnol. 38:282-290.