Low molecular weight rat fibroblast tropomyosin 5 (TM-5): cDNA cloning, actin-binding, localization, and coiled-coil interactions

Cell Motility and the Cytoskeleton

Volumn 33, Issue 3, 1996, Pages 223-240

  Temm Grove, Constance J ^a   Guo, Wei ^a   Helfman, David M ^a  

^a Howard Hughes Medical Institute Cold Spring Harbor Laboratory Cold Spring Harbor   (United States)

Author keywords

coiled coil; rat fibroblasts; TM 5; tropomyosin

Indexed keywords

ACTIN; COMPLEMENTARY DNA; TROPOMYOSIN;

ANIMAL CELL; ARTICLE; CELLULAR DISTRIBUTION; DNA LIBRARY; DNA SEQUENCE; FIBROBLAST; MACROPHAGE; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; RAT;

ACTINS; AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; BLOTTING, NORTHERN; BLOTTING, WESTERN; CELL LINE; CLONING, MOLECULAR; DNA, COMPLEMENTARY; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; FIBROBLASTS; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; RATS; RNA, MESSENGER; SEQUENCE HOMOLOGY, AMINO ACID; SEQUENCE HOMOLOGY, NUCLEIC ACID; TISSUE DISTRIBUTION; TROPOMYOSIN;

ANIMALIA; BACTERIA (MICROORGANISMS);

EID: 0029932698     PISSN: 08861544     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0169(1996)33:3<223::AID-CM6>3.0.CO;2-B     Document Type: Article

References (52)

1. Beisel, K.W., and Kennedy, J.E. (1994): Identification of novel alternatively spliced isoforms of the tropomyosin-encodmg gene, TMnm, in the rat cochlea. Gene 143:251-256.
2. Broschat, K O., and Burgess, D R. (1986): Low Mr tropomyosin isoforms from chicken brain and intestinal epithelium have distinct actin-binding properties. J. Biol. Chem. 261:13350-13359.
3. Brown, H.R., and Schachat, F.H. (1985): Renaturation of skeletal muscle tropomyosin: Implications for in vivo assembly. Proc. Natl. Acad. Sci. U.S.A. 82:2359-2363.
4. Chirgwin, J.M., Przybyla, A.E., MacDonald, R.J., and Rutter, W.J. (1979): Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18:5294-5299.
5. Clayton, L., Reinach, F.C., Chumbley, G.M., and MacLeod, A.R. (1988): Organization of the hTMnm gene: Implications for the evolution of muscle and nonmuscle tropomyosins. J. Biol. Chem, 264:2935-2944.
6. Forry-Schaudies, S., Maihle, N.J., and Hughes, S.H. (1990): Generation of skeletal, smooth and low molecular weight non-muscle tropomyosin isoforms from the chicken tropomyosin I gene. J. Mol. Biol. 211:321-330.
7. Gimona, M . Watakabe, A., and Helfman, D.M. (1995): Specificity of dimer formation in tropomyosins: Influence of alternatively spliced exons on homodimer and heterodimer assembly. Proc. Natl. Acad. Sci. U.S.A. 92:9776-9780.
8. Goodwin, L.O., Lees-Miller, J.P., Leonard, M.A., Cheley, S.B., and Helfman, D.M. (1991): Four fibroblast tropomyosin isoforms are expressed from the rat átropomyosin gene through the use of alternative promoters and alternative RNA processing. Mol. Cell. Biol. 10:1729-1742.
9. Heifman, D.M., Cheley, S., Kuismanen, E., Finn, L.A., and Yamawaki-Kataoka, Y. (1986): Nonmuscle and muscle tropomyosin isoforms are expressed from a single gene by alternative RNA splicing and polyadenylation. Mol. Cell. Biol. 6:3582-3595.
10. Ishikawa, R., Yamashiro, S., and Matsumura, F. (1989b): Annealing of gelsolin-severed actin filaments by tropomyosin in the presence of calcium. Potentiation of the annealing process by caldesmon. J. Biol. Chem. 264:16746-16770.
11. Ishikawa, R., Yamashiro, S., and Matsumura, F. (1989a): Differential modulation of actin-severing activity of gelsolin by multiple isoforms of cultured rat cell tropomyosin J. Biol. Chem. 264: 7490-7497
12. Jancso, A., and Graceffa, P. (1991): Smooth muscle tropomyosin coiled-coil dimers: Subunit composition, assembly, and end-to-end interaction. J. Biol Chem. 265:5891-5897.
13. Kornarska, M.M., Padgett, R.A., and Sharp. P.A. (1984): Recognition of cap structure in splicing in vitro mRNA precursors. Cell 38:731-736.
14. Laemmli, U.K. (1970): Cleavage of structural proteins during the assembly of the head of bacteriophage Tj. Nature 227.680-685.
15. Lees-Miller, J.P., and Helfman, D M. (1991): The molecular basis for tropomyosin isoform diversity. Bioessays 13:429-437.
16. Lees-Miller, J.P., Goodwin, L.O., and Helfman, D.M. (1990a): Three novel brain tropomyosin isoforms arc expressed from the rat α-tropomyosin gene through the use of alternative promoters and alternative RNA processing. Mol. Cell. Biol. 10:1729-1742.
17. Lees-Miller, J.P , Yan. A., and Helfman, D.M (1990b): Structure and complete nucleotide sequence of the gene encoding rat fibroblast tropomyosin 4. J. Mol. Biol. 213:399-405.
18. Lehrer, S.S., and Qian, Y. (1990). Unfolding/refolding studies of smooth muscle muscle tropomyosin. Evidence for a chain exchange mechanism in the preferential assembly of the native heterodimer. J. Biol. Chem. 265:1134-1138.
19. Lehrer, S.S., Qian, Y., and Hvidt, S. (1989): Assembly of the native heterodimer of Rana esculenta tropomyosin by chain exchange. Science 246:926-928.
20. Lin, J.J.-C., Helfman, D.M., Hughes, S.H., and Chou, C.-S (1985): Tropomyosin isoforms in chicken embryo fibroblasts: Purification, characterization, and changes in Rous sarcoma virus transformed cells. J. Cell Biol. 100:692-703.
21. Lin, J.J.-C., Hegmann, T.E., and Lin, J.L.-C. (1988): Differential localization of tropomyosin isoforms in cultured nonmuscle cells. J. Cell Biol. 107:563-572.
22. Lowry, O H., Rosenbrough, N.J., Farr, A.L., and Randall, R.J. (1951): Protein measurement with folm phenol reagent. J. Biol. Chem. 193:265-275.
23. nm, a human cytoskeletal tropomyosin. Nucleic Acids Res. 14:8413-8426.
24. Matsumura, F., and Yamashiro-Matsumura, S. (1985): Purification and characterization of multiple isoforms of tropomyosin from rat cultured cells. J. Biol. Chem. 260:13851-13859.
25. Matsumura, F., Lin, J.J.-C., Yamashiro-Matsumura, S., Thomas, G.P., and Topp, W.C. (1983): Differential expression of tropomyosin forms in the microfilaments isolated from normal and transformed rat cultured cells. J, Biol. Chem. 258:13954-13964.
26. Nishida, E., Muneyuki, E., Maekawa, S., Ohta, Y., and Sakai H. (1985): An actin-depolymerizing protein (destrin) from porcine kidney. Its action on F-actin containing or lacking tropomyosin. Biochemistry 24:6624-6630.
27. Novy, R.E., Liu, L.-F., Lin, C.S., Helfman, D.M., and Lin, J.J.-C. (1993a): Expression of smooth muscle and nonmuscie tropomyosins in E. coli and characterization of bactenally produced tropomyosins. Biochim. Biophys. Acta, 1162:255-265.
28. Novy, R.E., Sellers, J.R., Liu, L.-F., and Lin. J.J.-C. (1993b): In vitro functional characterization of bacterially expressed human fibroblast tropomyosin isoforms and their chimeric mutants. Cell Motil. Cytoskeleton 26:248-261.
29. O'Farrell, P.H. (1975); High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem. 250:4007-4021.
30. Pardee, J.D., and Spudich, J.A. (1982): Purification of muscle actin. Methods Enzymol. 85:164-181.
31. Pelham, H.R., and Jackson, R.J. (1976): An efficient mRNA-dependent translation system from reticulocyte lysates. Eur. J. Biochem. 67:247-256.
32. Pittenger, M.F., and Helfman, D.M. (1992): In vitro and in vivo characterization of four fibroblast tropomyosins produced in bacteria: TM-2, TM-3, TM-5a and TM-5b are colocalized in interphase fibroblasts. J. Cell Biol. 118:841-858.
33. Pittenger, M.F., Kazzaz, J.A., and Helfman, D.M. (1994): Functional properties of nonmuscle tropomyosin isoforms. Curr. Opin. Cell Biol. 6:96-104.
34. Pittenger, M.F., Kistler, A., and Helfman, D.M. (1995): Alternatively spliced exons of the β tropomyosin gene exhibit different affinities for F-actin and effects with nonmuscle caldesmon. J. Cell Sci. 108:3253-3265.
35. Prasad, G.L., Fuldner, R. A., and Cooper, H.L. (1993): Expression of transduced tropomyosin 1 cDNA suppresses neoplastic growth of cells transformed by the ras oncogene. Proc. Natl. Acad. Sci. U.S.A. 90:7039-7043.
36. Ruiz-Opazo, N., and Nadal-Ginard, B. (1987): α-Tropomyosin gene organization. J. Biol. Chem. 262:4755-4765.
37. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989): "Molecular Cloning: A Laboratory Manual." Cold Spring Harbor, NY: Cold Spring Harbor Press.
38. Sanger, F., Nicklen, S., and Coulson, A.R. (1977): DNA sequencing with chain termination inhibitors. Proc. Natl. Acad. Sci. U.S.A. 74:5463-5467.
39. Smillie, L. B. (1979): Structure and functions of tropomyosins from muscle and nonmtiscle sources. Trends Biochem. Sci. 4:151-54.
40. Soldati, T., and Perriard, J.-C. (1991): Intracompartmental sorting of essential myosin light chains: Molecular dissection and in vivo monitoring by epitope tagging. Cell 66:277-289.
41. Stamm, S., Casper, D., Lees-Miller, J.P., and Helfman, D.M. (1993): Brain-specific tropomyosins TMBr-1 and TMBr-3 have distinct patterns of expression during development and in adult brain. Proc. Natl. Acad. Sci. U.S.A. 90:9857-9861.
42. Studier, F.W., Rosenberg, A.M., Dunn, J.J., and Dubendorff, J.W. (1990): Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol. 185:60-89.
43. Sundell, C.C., and Singer, R.H. (1991): Requirement of microfilaments in sorting of actin mRNAs.b Science 253:1275-1277.
44. Takenaga, K., Nakamura, Y., Kageyama, H., and Sakiyama, S. (1990): Nucleotide sequence of cDNA for nonmuscle tropomyosin 5 of mouse fibroblast. Biochim. Biophys. Acta 1087:101-103.
45. Tanaka, M., and Herr, W. (1990): Differential transcriptional activation by oct-1 and oct-2: Interdependent activation domains induce oct-2 phosphorylation. Cell 63860:375-386.
46. Thierfelder, L.H. Watkins, MacRae, C., Lamas, R., McKenna, W., Vosberg, H.-P., Seidman, J.G., and Seidman, C.E. (1994): α-Tropomyosin and cardiac troponin T mutations cause familial hypertrophie cardiomyopathy: A disease of the sarcomere. Cell 77.701-712.
47. Wang, Y.-C., and Rubenstein, P.A. (1992): Splicing of two alternative exon pairs in β-tropomyosin pre-mRNA is independently controlled during myogenesis. J Biol. Chem. 267:12004-12010.
48. Weinberger, R.P., Henke, R.C., Tolhurst, O., Jeffrey, P.L., and Gunning, P. (1993): Induction of neuron-specific tropomyosin mRNAs by nerve growth factor is dependent on morphological differentiation. J. Cell Biol. 120:205-215.
49. Wieczorek, D.F., Smith, C.W.J., and Nadal-Ginard. B. (1988): The rat α-tropomyosin gene generates a minimum of six different mRNAs coding for striated, smooth, and nonmuscle isoforms by alternative splicing. Mol. Cell. Biol. 8:679-694.
50. Yamawaki-Kataoka, Y., and Helfman, D.M. (1985): Rat embryo fibroblast tropomyosin 1. J. Biol. Chem. 260:14440-14445.
51. Yamawaki-Kataoka, Y., and Helfman, D.M. (1987): Isolation and characterization of cDNA clones encoding a low molecular weight nonmuscle tropomyosin isoform. J. Biol. Chem. 262: 10791-10800.
52. Zinn, K., DiMaio, D., and Maniatis, T. (1983): Identification of two distinct regulatory regions adjacent to the human beta-interferon gene. Cell 34:865-879.