Computerized assessment of production of multiple human cytokines at the single-cell level using image analysis

Journal of Leukocyte Biology

Volumn 59, Issue 2, 1996, Pages 287-295

  Björk, Lars ^a,d   Fehniger, Thomas E ^a,b   Andersson, Ulf ^a,c   Andersson, Jan ^a,b  

^a STOCKHOLM UNIVERSITY   (Sweden)

^b KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

^c ST GÖRAN S HOSPITAL   (Sweden)

^d STOCKHOLM UNIVERSITY   (Sweden)

Author keywords

Image processing; Interferon ; Interleukin 2; Interleukin 1; Intracellulr staining; Measurement of cytokines

Indexed keywords

CYTOKINE; GAMMA INTERFERON; INTERLEUKIN 1; INTERLEUKIN 2;

ARTICLE; ASSAY; COMPUTER ANALYSIS; CONTROLLED STUDY; HUMAN; HUMAN CELL; IMAGE ANALYSIS; IMMUNOCYTOCHEMISTRY; MONONUCLEAR CELL; NORMAL HUMAN; PRIORITY JOURNAL;

EID: 0029932157     PISSN: 07415400     EISSN: None     Source Type: Journal    
DOI: 10.1002/jlb.59.2.287     Document Type: Article

References (19)

1. Andersson, U., Halldén, G., Persson, U., Hed, J., Möller, C., DeLey, M. (1988) Enumeration of IFN-gamma-producing cells by flow cytometry: comparison with fluorescence microscopy. J. Immunol. Meth. 112, 139-142.
2. Jung, T., Schauer, U., Hausser, C., Neumann, C., Rieger, C. (1993) Detection of intracellular cytokines by flow cytometry. J. Immunol. Meth. 159, 197-207.
3. Assenmacher, M., Schmitz, J., Radbruch, A. (1994) Flow cytometric determination of cytokines in activated marine T helper lymphocytes: expression of interleukin-10 and interferon-γ and interleukin-4 expressing cells. Eur. J. Immunol. 24, 1097-1101.
4. Sander, B., Andersson, J., Andersson, U. (1991) Assessment of cytokines by immunofluorescence and the paraformaldehyde-saponin procedure. Immunol. Rev. 119, 65-93.
5. Henter, J-I., Söder, O., Andersson, U. (1988) Identification of individual tumor necrosis factor/cachectin-producing cells after lipopolysaccharide induction. Eur. J. Immunol. 18, 983-988.
6. Doulhain, R.J.E.M., Andersson, U., ter Haar, N.T., Brinkman, B.M.N., Verweij, C.L., Daha, M.R., Breedveld, F.C., Miltenburg, A.M.M. (1993) Detection of intracellular interferon-γ by light microscopy using an immunoperoxidase technique: correlation with the corresponding mRNA and protein product. J. Leukoc. Biol. 54, 545-551.
7. Andersson, J., Björk, L., Dinarello, C., Andersson, U. (1992) Lipopolysaccharide induces human interleukin-1 receptor antagonist, and interleukin-1 production in the same cell. Eur. J. Immunol. 22, 2617-2623.
8. Björk, L., Andersson, U., Chauvet, J-M., Skansen-Saphir, U., Andersson, J. (1994) Quantification of superantigen induced IFN-γ production by computerised image analysis-inhibition of cytokine production and blast transformation by pooled human IgG. J. Immunol. Meth. 175, 201-213.
9. Björk, L., Andersson, J., Ceska, M., Andersson, U. (1992) Endotoxin and Staphylococcus aureus enterotoxin A induce different patterns of cytokines. Cytokine 4, 513-519.
10. Andersson, U., Andersson, J., Lindfors, A., Wagner, K., Möller, G., Heusser, C. (1990) Simultaneous production of interleukin 2, interleukin 4 and interferon gamma by activated human blood lymphocytes. Eur. J. Immunol. 20, 1591-1596.
11. Issekutz, A.C. (1983) Removal of gram-negative endotoxin from solutions by affinity chromatography. J. Immunol. Meth. 61, 275-281.
12. Schneider, H., Vogt, A., Bross, K. (1984) Identification of proliferating subpopulations in microcultures by surface marker and autoradiography. Immunol. Commun. 13, 553-561.
13. Rubartelli, A., Cozzolino, F., Talio, M., Sitia, R. (1990) A novel pathway for interleukin 1β, a protein lacking a signal sequence. EMBO J. 9, 1503-1510.
14. Inoue, S. (1987) Video Microscopy. Plenum Press, New York.
15. Land, E.H., McCann, J.J. (1971) Lightness and retinex theory. J. Opt. Soc. Am. 61, 1-11.
16. Ferrick, D.A., Schrenzel, M.D., Mulvania, T., Hsieh, B., Ferlin, W.G., Lepper, H. (1995) Differential production of interferon-γ and interleukin-4 in response to Th1- and Th2-stimulating pathogens by γδ T cells in vivo. Nature 373, 255-257.
17. Andersson, J., Nagy, S., Björk, L., Abrams, J., Holm, S., Andersson, U. (1992) Bacterial toxin induced cytokine production studied at the single cell level. Immunol. Rev. 127, 69-96.
18. Navikas, V., Haglund, M., Link, J., He, B., Lindqvist, L., Link, H. (1995) Cytokine mRNA profiles in mononuclear cells in acute aseptic meningoencephalitis. Infect. Immunol. 63, 1581-1586.
19. Fernandez, V., Andersson, J., Andersson, U., Troye-Blomberg, M. (1994) Cytokine synthesis analyzed at the single-cell level before and after revaccination with tetanus toxoid. Eur. J. Immunol. 24, 1808-1815.