Laboratory implementation of a rapid three-stain technique for detection of microorganisms from lower respiratory specimens

Journal of Clinical Laboratory Analysis

Volumn 10, Issue 2, 1996, Pages 104-109

  Maymind, Michael ^a   Thomas, John G ^a   Abrons, Henry L ^b   Riley, Roger S ^a  

^a WEST VIRGINIA UNIVERSITY   (United States)

^b WEST VIRGINIA UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

bronchoalveolar lavage; calcofluor white; immunocompromised patient; Pneumocystis carinii

Indexed keywords

ARTICLE; BACTERIUM DETECTION; CONTROLLED STUDY; COST EFFECTIVENESS ANALYSIS; DIAGNOSTIC ACCURACY; DIAGNOSTIC PROCEDURE; DIAGNOSTIC VALUE; FEMALE; HUMAN; HUMAN CELL; LOWER RESPIRATORY TRACT INFECTION; LUNG LAVAGE; MAJOR CLINICAL STUDY; MALE; PRACTICE GUIDELINE; PREDICTION; STAINING;

ADULT; AGED; ASPERGILLOSIS; ASPERGILLUS FUMIGATUS; BRONCHOALVEOLAR LAVAGE; CANDIDIASIS; CELL SIZE; CHILD, PRESCHOOL; FEMALE; GRAM-POSITIVE BACTERIA; HUMANS; MALE; MIDDLE AGED; PNEUMONIA, PNEUMOCYSTIS; STAINING AND LABELING;

BACTERIA (MICROORGANISMS); FUNGI; PNEUMOCYSTIS CARINII; POSIBACTERIA;

EID: 0029932119     PISSN: 08878013     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1098-2825(1996)10:2<104::AID-JCLA8>3.0.CO;2-C     Document Type: Article

References (19)

1. Baughman RP, Dohn MN: Immunocompromised Patients. In Bronchoalveolar Lavage, RP Baughman, ed. Mosley Year Book, St. Louis, 1992, p 41-63.
2. Bedrossian CWM, Mason MR, Gupta PK: Rapid cytologic diagnosis of pneumocystis: A comparison of effective techniques. Semin Diag Pathol 6:245-261, 1989.
3. Delvenne P, Arrese JE, Thiry A, Borlee-Hermans G, Pierard GE, Boniver J: Detection of cytomegalovirus, Pneumocystis carinii, and Aspergillus species in bronchoalveolar lavage fluid: A comparison of techniques. Am J Clin Pathol 100:414-418, 1993.
4. Raab SS, Cheville JC, Bottles K, Cohen MB: Utility of Gomori methenamine silver strains in bronchoalveolar lavage specimens. Mod Pathol 7:599-604, 1994.
5. Schumann GB, Swensen JJ: Comparison of Papanicolaou's stain with the gomon methenamine silver (GMS) stain for the cytodiagnosis of Pneumocystis carinii in bronchoalveolar lavage (BAL) fluid. Am J Clin Pathol 95:583-586, 1991.
6. Tegnago R, Xavier RG, Pereira RP, Prolla JC: The diagnosis of Pneumocystis carinii pneumonia by cytologic evaluation of Papanicolaou and Leishman-stamed bronchoalveolar specimens in patients with the acquired immunodeficiency syndrome Cytopathology 4:77-84, 1993.
7. Ng VL, Virani NA, Chaisson RE, Yajko DM, Sphar HT, Cabrian K, Rollins N, Charache P, Krieger M, Hadley WK, Hopewell PC: Rapid detection of Pneumocystic carinii using a direct fluorescent monoclonal antibody stain. J Clin Microbiol 28:2228-2233, 1990.
8. Tollerud DJ, Wesseler TA, Kim CK, and Baughman RP: Use of rapid differential stain for identifying Pneumocystis carinii in bronchoalveolar lavage fluid. Chest 95:494-497, 1989.
9. Schumann GB, Linker G: Cytopreparatory techniques for bronchoalveolar lavage specimens. Lab Med 23:115-119, 1992.
10. Baselski VS, Robinson MK, Pifer LW, Woods DR: Rapid detection of Pneumocystis carimi in bronchoalveolar lavage samples by using Cellufluor staining. J Clin Microbiol 28:393-394, 1990.
11. Stratton N, Hryniewiski J, Aarnaes SL, Tan G, de la Maza LM, Peterson EM: Comparison of monoclonal antibody and Calcofluor white stains for the detection of Pneumocystis carinii from respiratory specimens. J Clin Microbiol 29:645-647, 1991.
12. Monheit JE, Cowan DF, Moore DG: Rapid detection of fungi in tissues using calcofluor white and fluorescence microscopy. Arch Pathol Lab Med 108:616-618, 1984.
13. Monheit JG, Brown G, Kott MM, et al.: Calcofluor white detection of fungi in cytopathology. Am J Clin Pathol 85:222-225, 1986.
14. Marines HM, Osato MS, Font RL: The value of calcofluor white in the diagnosis of mycotic and Acanthamoeba infections of the eye and ocular adnexa. Ophthalmology 94:23-26, 1987.
15. Wachsmuth ED: A comparison of the highly selective fluorescence staining of fungi in tissue sections with Uvitex 2B and Calcofluor White M2R. Histochem J 20:215-221, 1988.
16. Greenberg SR: The comparative histologic application of Phorwite Fabric brighteners. J Histotechnol 12:43-45, 1989.
17. Kim YK, Parulekar S, Yu PK, Pisani RJ, Smith TF and Anhalt JP: Evaluation of Calcofluor white stain for detection of Pneumocystis carinii. Diagn Microbiol Infect Dis 13:307-310, 1990.
18. Stager CE, Fraire AE, Kim HS, Estrada R, Davis JR, Greenberg D, Guy ES, Guntypalli KK, McBride RA: Modification of the fungi-fluor and the genetic systems fluorescent antibody methods for detection of Pneumocystis carinii in bronchoalveolar lavage specimens. Arch Pathol Lab Med 119:142-147, 1995.
19. Harrington BJ, Hageage CJ: Calcofluor white: Tips for improving its use. Clin Microbiol Newsletter 13:3-5, 1991.