A quantitative method of determining initial amounts of DNA by polymerase chain reaction cycle titration using digital imaging and a novel DNA stain

Analytical Biochemistry

Volumn 237, Issue 2, 1996, Pages 204-207

  Becker, Andreas ^a   Reith, Annette ^a   Napiwotzki, Jörg ^a   Kadenbach, Bernhard ^a  

^a PHILIPPS UNIVERSITY MARBURG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; IMAGE RECORDING; VIDEO CAMERAS;

DIGITAL IMAGING; FLUORESCENT DYES; GRAPHICAL METHODS; MITOCHONDRIAL DNA; NONRADIOACTIVE; QUANTITATIVE DETERMINATIONS; QUANTITATIVE METHOD; SERIAL DILUTIONS;

POLYMERASE CHAIN REACTION;

MITOCHONDRIAL DNA;

ARTICLE; COMPUTER PROGRAM; DIAGNOSTIC ACCURACY; DIAGNOSTIC IMAGING; DIGITAL COMPUTER; DNA DETERMINATION; FLUORESCENCE; HUMAN; HUMAN TISSUE; IMAGE ANALYSIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; VIDEORECORDING;

EID: 0029931435     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.1996.0230     Document Type: Article

References (16)

1. Coral-Debrinski, M., Stepien, G., Shoffner, J. M., Lott, M. T., Kanter, K., and Wallace, D. C. (1991) J. Am. Med. Assoc. 266, 1812-1816.
2. Ozawa, T., Tanaka, M., Ikebe, S., Ohno, K., Kondo, T., and Mizuno, Y. (1990) Biochem. Biophys. Res. Commun. 172, 483-489.
3. Sugiama, S., Hattori, K., Hayakawa, M., and Ozawa, T. (1991) Biochem. Biophys. Res. Commun. 180, 894-899.
4. Hayakawa, M., Hattori, K., Sugiyama, S., and Ozawa, T. (1992) Biochem. Biophys. Res. Commun. 189, 979-985.
5. Simonetti, S., Chen, X., DiMauro, S., and Schon, E. A. (1992) Biochim. Biophys. Acta 1180, 113-122.
6. Chen, X., Prosser, R., Simonetti, S., Sadlock, J., Jagiello, G., and Schon, E. A. (1995) Am. J. Hum. Genet. 57, 239-247.
7. Mann, V. M., Cooper, J. M., and Schapiro, A. H. V. (1992) FEBS Lett. 299, 218-222.
8. Celi, F. S., Zenilman, E., and Shuldiner, A. R. (1993) Nucleic Acids Res. 21, 1047.
9. Hamblet, N. S., and Castora, F. J. (1995) Biochem. Biophys. Res. Commun. 207, 839-847.
10. Becker, A., Napiwotzki, J., Damian, M., and Kadenbach, B. (1995) Med. Genet. 7(2), B-19.
11. Wallace, D. C., Zheng, X., Lott, M. T., Shoffner, J. M., Hodge, J. A., Kelley, R. I., Epstein, C. M., and Hopkins, L. C. (1988) Cell 55, 601-610.
12. Anderson, S., Bakier, A. T., Barrell, B. G., deBrujin, M. H. L., Coulson, A. R., Drouin, J., Eperon, I. C., Nierlich, D. P., Roe, B. A., Sanger, F., Schreier, P. H., Smith, A. J. H., Stade, R., and Young, I. G. (1981) Nature 290, 457-465.
13. Cortopassi, G. A., and Arnheim, N. (1990) Nucleic Acids Res. 18, 6927-6933.
14. Münscher, C., Müller-Höcker, J., and Kadenbach, B. (1993) Biol. Chem. Hoppe-Seyler 374, 1099-1104.
15. Wallace, D. C., Bohr, V. A., Cortopassi, G., Kadenbach, B., Linn, S., Linnane, A. W., Richter, C., and Shay, J. W. (1995) in Molecular Aspects of Aging (Esser, K., and Martin, G. M., Eds.), pp. 199-225, Dahlem Workshop Report LS 56, Wiley, Chichester.
16. Kadenbach, B., Münscher, C., Frank, V., Müller-Höcker, J., and Napiwotzki, J. (1995) Mutat. Res. 338, 161-172.