Joining of DNA fragments by repeated cycles of denaturation, annealing and extension

BioTechniques

Volumn 20, Issue 6, 1996, Pages 954-955

  Kim, Jaehong ^a   Puder, Mark ^a   Soberman, Roy J ^a  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT;

ARTICLE; DENATURATION; DNA LIBRARY; ENZYME INACTIVATION; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION;

EID: 0029929087     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96206bm01     Document Type: Article

References (6)

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2. Chirgwin, J.J., A.E. Pryzbyla, RJ. MacDonald and W.J. Rutter. 1979. Isolation of biologically active ribonucleic acid from sources rich in ribonuclease. Biochemistry 18:5294-5299.
3. Kikiuta, Y., E. Kusunose, K. Endo, S. Yamamoto, K. Sogawa, Y. Fuji-Kuriyama and M. Kusonose. 1993. A novel form of cytochrome P-450 family in human polymorphonuclear leukocytes. J. Biol. Chem. 268:9376-9380.
4. Powell, W.S. 1984. Properties of leukotriene B4 20-hydroxylase from polymorphonuclear leukocytes. J. Biol. Chem. 259:3082-3089.
5. 4 omega hydroxylase in human polymorphonuclear leukocytes. Partial purification and identification as a cytochrome P-450. J. Clin. Invest. 76:1218-1228.
6. 4 20-hydroxylase in human polymorphonuclear leukocytes. Proc. Natl. Acad. Sci. USA 82:2292-2295.