Cytokine activity assay by means of proliferation measured in plane convex microtiter wells

Journal of Biochemical and Biophysical Methods

Volumn 32, Issue 2, 1996, Pages 85-96

  Vorauer, Karola ^a   Steindl, Franz ^a   Jungbauer, Alois ^a   Hahn, Rainer ^a   Katinger, Hermann ^a  

^a UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

Author keywords

3 (4,5 Dimethylthiazol 2 yl) 2,5 diphenyltetrazolium bromide; 3 (4,5 Dimethylthiazol 2 yl) 5 (3 carboxymethoxyphenyl) 2 (4 sulfophenyl) 2H tetrazolium; Cytotoxic T cell line; General cell screening system; Interleukin 2; Proliferation assay

Indexed keywords

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; CYTOKINE; INTERLEUKIN 2;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; CYTOTOXIC T LYMPHOCYTE; LYMPHOCYTE PROLIFERATION; MITOSIS RATE; MOUSE; NONHUMAN; PRIORITY JOURNAL; SPECTROPHOTOMETRY; T LYMPHOCYTE ACTIVATION;

ANIMALIA;

EID: 0029927088     PISSN: 0165022X     EISSN: None     Source Type: Journal    
DOI: 10.1016/0165-022X(96)00002-4     Document Type: Article

References (11)

1. Mosmann, T., Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods, 65 (1983) 55-63.
2. Tada, H., Shiho, O., Kuroshima, K., Koyama, M. and Tsukamoto, K., An improved colorimetric assay for Interleukin-2. J. Immunol. Methods, 93 (1986) 157-165.
3. Hewlett, G., Stünkel, K.G. and Schlumberger, H.D., A method for quantification of Interleukin-2 activity. J. Immunol. Methods, 117 (1989) 243-246.
4. Robb, R.J., Kutny, R.M., Panico, M., Morris, H.R. and Chowdhry, V., Amino acid sequence and post-translational modification of human interleukin-2. Proc. Natl. Acad. Sci. USA, 81 (1984) 6486-6490.
5. Strahel, R.A., Klinische Bedeutung von Interleukin-2 in der Tumortherapie. Prakt. Onkol., 8 (1989) 126-129.
6. Ju, G., Collins, L., Kaffka, K.L., Tsien, W., Chizzonite, R., Crowl, R., Bhatt, R. and Kilian, P.L., Structure-Function Analysis of Human Interleukin-2. J. Biol. Chem., 262 (1987) 5733-5731.
7. Buttke, T.M., McCubrey, J.A. and Owen, T.C., Use of an aqueous soluble tetrazolium/formazan assay to measure viability and proliferation of lymphokine-dependent cell lines. J. Immunol. Methods. 157 (1993) 233-240.
8. Barltrop, J.A. and Owen, T.C., 5-(3-Carboxymethoxyphenyl)-2-(4,5-dimethylthiazolyl)-3-(4-sulphophenyl) tetrazolium, Inner Salt (MTS) and related analogs of 3-(4,5-dimethylthiazolyl)-2,5-diphenyltetrazolium bromide (MTT) reducing to purple water-soluble formazans as cell-viability indicators. Bioorg. Med. Chem. Lett., 1 (1991) 611-614.
9. Steindl, F., General cell screening system-a new dimension in screening and cell culture, Fermentation and Cell Culture/Application Note, Int. Biotechnology Laboratory, 1990.
10. Gearing, A.J.H. and Bird, C.M., Production and Assay of Interleukin-2, Lymphokines and Interferons: A Practical Approach. IRL Press, Oxford 291, 1987.
11. Gillis, S., Ferm, M.M., Ou, W. and Smith, K.A., T cell growth factor: parameters of production and a quantitative microassay for activity, J. Immunol. Methods 120 (1978) 2027.