Determining the site and nature of DNA mutations with the cloned MutY mismatch repair enzyme

Carcinogenesis

Volumn 17, Issue 2, 1996, Pages 321-326

  Xu, Jing Fan ^a   Yang, Qiu Ping ^a   Chen, James Y J ^a   Van Baalen, Marrit R ^a   Hsu, Ih Chang ^a  

^a UNIVERSITY OF MARYLAND SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ENZYME; ISOPROPYL THIOGALACTOSIDE; MUTANT PROTEIN; POLYDEOXYRIBONUCLEOTIDE SYNTHASE; PROTEIN P53;

ARTICLE; BACTERIAL GENE; BASE MISPAIRING; CONTROLLED STUDY; DNA CLEAVAGE; DNA REPAIR; DNA SEQUENCE; DNA TRANSFECTION; ENZYME ACTIVITY; ESCHERICHIA COLI; EXPRESSION VECTOR; GEL FILTRATION CHROMATOGRAPHY; ION EXCHANGE CHROMATOGRAPHY; MOLECULAR CLONING; NONHUMAN; PLASMID; POINT MUTATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN PURIFICATION; TUMOR; TUMOR SUPPRESSOR GENE;

BASE SEQUENCE; DNA GLYCOSYLASES; DNA LIGASES; DNA MUTATIONAL ANALYSIS; ENZYME INDUCTION; ESCHERICHIA COLI; GENES, BACTERIAL; HUMANS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; N-GLYCOSYL HYDROLASES; POINT MUTATION; TUMOR SUPPRESSOR PROTEIN P53;

EID: 0029927002     PISSN: 01433334     EISSN: None     Source Type: Journal    
DOI: 10.1093/carcin/17.2.321     Document Type: Article

References (23)

1. Saiki,R.K., Scherf,S., Faloona,F., Mullis,K.B., Horn,G.T., Erlich,H.A. and Armheim,N. (1988) Primer-directed enzymatic amplification of DNA with thermostable DNA polymerase. Science, 239, 487-491.
2. Sanger,F., Nicklen,S. and Coulson,A.R. (1977) DNA sequencing chain terminating inhibitors. Proc. Nail Acad. Sci. USA, 74, 5463-5467.
3. Hollstein,M., Sidransky,D., Vogelstein,B. and Harris,C.C. (1991) p53 mutations in human cancers. Science, 253, 49-53.
4. Lishanski,A., Ostrander,E.A. and Rine,J. (1994) Mutation detection by mismatch binding protein, MutS, in amplified DNA: application to the cystic fibrosis gene. Proc. Natl Acad. Sci. USA, 91, 2674-2678.
5. Ellis,L.A., Taylor,G.R. and Baumberg,S. (1994) MutS binding protects heteroduplex DNA from exonuclease digestion in vitro: a simple method for detecting mutations. Nucleic Acids Res., 22, 2710-2711.
6. Youi,U.R., Kemper,B.W. and Cotton,R.G.H. (1995) Screening for mutations by enzyme mismatch cleavage with T4 endonuclease VII. Proc. Natl Acad. Sci. USA, 92, 87-91.
7. Mashal,R.D., Koontz,J. and Splar,J. (1995) Detection of mutations by cleavage of DNA heteroduplexes with bacteriophage resolvase. Nature Genet., 9, 177-183.
8. Murakami,Y., Hayashi,K. and Sekiya,T. (1991) Detection of aberration of the p53 alleles and the gene transcript in human tumor cell lines by single-strand conformation polymorphism analysis. Cancer Res., 51, 3356-3361.
9. s-globin allele by hybridization with synthetic oligonucleotides. Proc. Natl Acad. Sci. USA, 80, 278-282.
10. Myers,R.M., Maniatis,T. and Lehman,L.S. (1987) Detection and localization of single base changes by denaturing gradient gel electrophoresis. Methods Enzymol., 155, 501-527.
11. Myers,R.M., Larin,Z. and Maniatis,T. (1985) Detection of single base substitutions by ribonuclease cleavage at mismatches in RNA:DNA duplexes. Science, 230, 1242-1246.
12. Hsu,I.C., Yang,Q.R. Kahng,M.W. and Xu,J.F. (1994) Detection of DNA point mutations with DNA mismatch repair enzymes. Carcinogenesis, 15, 1657-1662.
13. Au,K.G., Clark,S., Miller,J.H. and Modrich,P. (1989) E.coli mut Y gene encodes an adenine glycosylase active on G:A mispairs. Proc. Nail Acad. Sci. USA. 86, 8877-8881.
14. Wiebauer,K. and Jiricny,J. (1989) In vitro correction of G:T mispairs to G:C pairs in nuclear extracts from human cells. Nature, 339, 234-236.
15. Michaels,M.L., Pham,L., Nghiem,Y., Cruz,C. and Miller,J.H. (1989) MutY, an adenine glycosylase active on G/A mispairs, has homology to endonuclease III. Nucleic Acids Res., 18, 3841-3844.
16. Neddermann,P. and Jiricny,J. (1993) The purification of a mismatchspecific thymine-DNA glycosylase from HeLa cells. J. Biol. Chem., 268, 21218-21224.
17. Studier,F.W., Rosenberg,A.H., Dunn,J.J. and Dubendorff,J.W. (1990) Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol., 185, 60-89.
18. Hsu,J.C., Walsh,J., Takiwa,T., Metcalf,R. and Harris,C.C. (1993) p53 mutation, hepatitis B virus DNA sequences in human liver cancer cells. Carcinogenesis, 14, 987-992.
19. Lu,A. and Hsu,I.C. (1992) Detection of single DNA base mutations with mismatch repair enzymes. Genomics, 14, 249-255.
20. Hsu,I.C., Metcalf,R.A., Sun,T., Welsh,J. and Harris,C.C. (1991) p53 gene mutational hotspot in human hepatocellular carcinoma from Quidong, China. Nature, 350, 427-428.
21. Holmaes,J., Clark,S. and Modrich,P. (1990) Strand-specific mismatch correction in nuclear extracts of human and Drosophila melanogaster cell lines. Proc. Nail Acad. Sci. USA. 87, 5837-5841.
22. Muster-Nassal,C. and Kolodner,R. (1986) Mismatch correction catalyzed by cell free extracts of Saccharomyces cerevisiae. Proc. Natl Acad. Sci. USA, 83, 7618-7622.
23. Tsai-Wu,J., Liu,H. and Lu,A. (1992) E.coli mut Y protein has both N-glycoslyase and endonuclease activities on A/C and A/G mispairs. Proc. Nail Acad. Sci. USA, 89, 8779-8783.