Insulin sensitizes β-agonist and forskolin-stimulated lipolysis to inhibition by 2′,5′-dideoxyadenosine

American Journal of Physiology - Cell Physiology

Volumn 270, Issue 2 39-2, 1996, Pages

  Gokmen Polar, Yesim ^a   Coronel, Elizabeth C ^a   Bahouth, Suleiman W ^a   Fain, John N ^a  

^a UNIVERSITY OF TENNESSEE HEALTH SCIENCE CENTER   (United States)

Author keywords

Adenosine 3 ,5 cyclic monophosphate; Adipocytes; Catecholamines; Protein kinase A

Indexed keywords

2',5' DIDEOXYADENOSINE; 5 [2 [[2 (3 CHLOROPHENYL) 2 HYDROXYETHYL]AMINO]PROPYL] 1,3 BENZODIOXOLE 2,2 DICARBOXYLIC ACID; ADENOSINE TRIPHOSPHATE; BETA ADRENERGIC RECEPTOR STIMULATING AGENT; CATECHOLAMINE; CYCLIC AMP; CYCLIC AMP DEPENDENT PROTEIN KINASE; FORSKOLIN; GLUCOSE; INSULIN; ISOPRENALINE; NORADRENALIN; PHENYLISOPROPYLADENOSINE;

ADIPOCYTE; ANIMAL CELL; ARTICLE; CONCENTRATION RESPONSE; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME ACTIVITY; LIPID METABOLISM; LIPOLYSIS; NONHUMAN; PRIORITY JOURNAL; RAT;

ADIPOCYTES; ADRENERGIC BETA-AGONISTS; ANIMALS; CATECHOLAMINES; CELLS, CULTURED; CYCLIC AMP; CYCLIC AMP-DEPENDENT PROTEIN KINASES; DIDEOXYADENOSINE; DIOXOLES; DRUG SYNERGISM; FORSKOLIN; INSULIN; LIPOLYSIS; RATS; RATS, SPRAGUE-DAWLEY;

EID: 0029925884     PISSN: 03636143     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajpcell.1996.270.2.c562     Document Type: Article

References (30)

1. Allen, D. O., B. Ahmed, and K. Naseer. Relationships between cyclic AMP levels and lipolysis in fat cells after isoproterenol and forskolin stimulation. J. Pharmacol. Exp. Ther. 238: 659-664, 1986.
2. Allen, D. O., and J. T. Quesenberry. Quantitative differences in the cyclic AMP-lipolysis relationships for isoproterenol and forskolin. J. Pharmacol. Exp. Ther. 244: 852-859, 1988.
3. 3-adrenergic agonist. Drugs Future 19: 23-26, 1994.
4. Boobis, L. H., and R. J. Maughan. A simple one-step enzymatic fluorometric method for the determination of glycerol in 20 μl of plasma. Clin. Chim. Acta 132: 173-179, 1983.
5. Brown, B. L., J. D. M. Albano, R. P. Ekins, A. M. Sgherzi, and W. Tampion. A simple and sensitive saturation assay method for the measurement of adenosine 3′:5′-cyclic monophosphate. Biochem. J. 121: 561-562, 1971.
6. Dalziel, H. H., and D. P. Westfall. Receptors for adenine nucleotides and nucleosides: subclassification, distribution, and molecular characterization. Pharmacol. Rev. 46: 449-466, 1994.
7. 6-(phenylisopropyl)adenosine and 2′,5′-dideoxyadenosine. J. Cyclic Nucleotide Res. 5: 189-196, 1979.
8. Fain, J. N., and C. C. Malbon. Regulation of adenylate cyclase by adenosine. Mol. Cell. Biochem. 25: 143-169, 1979.
9. Fain, J. N., R. H. Pointer, and W. F. Ward. Effects of adenosine nucleosides on adenylate cyclase, phosphodiesterase, cyclic adenosine monophosphate accumulation, and lipolysis in fat cells. J. Biol. Chem. 247: 6866-6872, 1972.
10. Fraundorfer, P. F., R. H. Fertel, D. D. Miller, and D. R. Feller. Biochemical and pharmacological characterization of high affinity trimetoquinol analogs on guinea pig and human β-adrenergic receptor subtypes: evidence for partial agonism. J. Pharmacol. Exp. Ther. 270: 665-674, 1994.
11. 3 adrenergic receptors to adenylyl cyclase in isolated adipocytes. J. Pharmacol. Exp. Ther. 261: 638-642, 1992.
12. Hollenga, C., F. Brouwer, and J. Zaagsma. Relationship between lipolysis and cyclic AMP generation mediated by atypical β-adrenoceptors in rat adipocytes. Br. J. Pharmacol. 102: 577-580, 1991.
13. Hollenga, C., F. Brouwer, and J. Zaagsma. Differences in functional cyclic AMP compartments mediating lipolysis by isoprenaline and BRL 37344 in four adipocyte types. Eur. J. Pharmacol. 200: 325-330, 1991.
14. Honnor, R. C., G. S. Dhillon, and C. Londos. cAMP-dependent protein kinase and lipolysis in rat adipocytes. J. Biol. Chem. 260: 15122-15129, 1985.
15. Honnor, R. C., G. S. Dhillon, and C. Londos. cAMP-dependent protein kinase and lipolysis in rat adipocytes. J. Biol. Chem. 260: 15130-15138, 1985.
16. Johnson, R. A., and I. Shoshani. Kinetics of "P"-site-mediated inhibition of adenylyl cyclase and the requirements for substrate. J. Biol. Chem. 265: 11595-11600, 1990.
17. Kublaoui, B., J. Lee, and P. F. Pilch. Dynamics of signaling during insulin-stimulated endocytosis of its receptor in adipocytes. J. Biol. Chem. 270: 59-65, 1995.
18. Kuroda, M., R. C. Honnor, S. W. Cushman, C. Londos, and I. A. Simpson. Regulation of insulin-stimulated glucose transport in the isolated rat adipocyte. J. Biol. Chem. 262: 245-253, 1987.
19. 3 adrenergic receptors expressed in native fat cells and in transfected cell lines. Cell. Signal. 6: 363-392, 1994.
20. 3-adrenergic receptors parallels lipolysis in rat adipocytes. Biochim. Biophys. Acta 1135: 349-352, 1992.
21. Litosch, I., T. H. Hudson, I. Mills, S. Y. Li, and J. N. Fain. Forskolin as an activator of cyclic AMP accumulation and lipolysis in rat adipocytes. Mol. Pharmacol. 22: 109-115, 1982.
22. Londos, C., D. M. F. Cooper, W. Schlegel, and M. Rodbell. Adenosine analogs inhibit adenylate cyclase by a GTP-dependent process: basis for actions of adenosine and methylxanthines on cyclic AMP production and lipolysis. Proc. Natl. Acad. Sci. USA 75: 5362-5366, 1978.
23. Londos, C., and J. Wolff. Two distinct adenosine-sensitive sites on adenylate cyclase. Proc. Natl. Acad. Sci. USA 74: 5482-5486, 1977.
24. Murphy, G. J., D. M. Kirkham, M. A. Cawthorne, and P. Young. Correlation of β3-adrenoceptor-induced activation of cyclic AMP-dependent protein kinase with activation of lipolysis in rat white adipocytes. Biochem. Pharmacol. 46: 575-581, 1993.
25. Murray, K. J., P. J. England, J. A. Lynham, D. Mills, C. Schmitz-Peiffer, and M. L. Reeves. Use of a synthetic dodecapeptide (malantide) to measure the cyclic AMP-dependent protein kinase activity ratio in a variety of tissues. Biochem. J. 267: 703-708, 1990.
26. Okada, T., Y. Kawano, T. Sakakibara, O. Hazeki, and M. Ui. Essential role of phosphatidylinositol 3-kinase in insulin-induced glucose transport and antilipolysis in rat adipocytes. J. Biol. Chem. 269: 3568-3573, 1994.
27. Salomon, Y., C. Londos, and M. Rodbell. A highly sensitive adenylate cyclase assay. Anal. Biochem. 58: 541-548, 1974.
28. Schimmel, R. J. Stimulation of cAMP accumulation and lipolysis in hamster adipocytes with forskolin. Am. J. Physiol. 246 (Cell Physiol. 15): C63-C68, 1984.
29. Taussig, R., and A. G. Gilman. Mammalian membrane-bound adenylyl cyclases. J. Biol. Chem. 270: 1-4, 1995.
30. Van Liefde, I., A. Van Witzenburg, and G. Vauquelin. Multiple beta adrenergic receptor subclasses mediate the isoproterenol-induced lipolytic response in rat adipocytes. J. Pharmacol. Exp. Ther. 262: 552-558, 1992.