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Volumn 20, Issue 4, 1996, Pages 662-668

Total protein extraction from cultured cells for use in electrophoresis and Western blotting

Author keywords

[No Author keywords available]

Indexed keywords

ACTIN; CELL PROTEIN; PROTEIN ANTIBODY; PROTEIN KINASE C; TRICHLOROACETIC ACID;

EID: 0029925236     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article
Times cited : (41)

References (18)
  • 1
    • 0025272557 scopus 로고
    • A procedure for the extraction and high resolution two-dimensional gel electrophoresis of total nuclear phosphoproteins from isotonically purified nuclei
    • Baciu, P.C. and J.P. Durham. 1990. A procedure for the extraction and high resolution two-dimensional gel electrophoresis of total nuclear phosphoproteins from isotonically purified nuclei. Electrophoresis 11:162-166.
    • (1990) Electrophoresis , vol.11 , pp. 162-166
    • Baciu, P.C.1    Durham, J.P.2
  • 2
    • 0027318996 scopus 로고
    • Sequence and functional characterization of a third inositol triphosphate receptor subtype, IP3R-3, expressed in pancreatic islets, kidney, gastrointestinal tract and other tissues
    • Blondel, O., J. Takeda, H. Janssen, S. Seino and G.I. Bell. 1993. Sequence and functional characterization of a third inositol triphosphate receptor subtype, IP3R-3, expressed in pancreatic islets, kidney, gastrointestinal tract and other tissues. J. Biol. Chem. 268:11356-11363.
    • (1993) J. Biol. Chem. , vol.268 , pp. 11356-11363
    • Blondel, O.1    Takeda, J.2    Janssen, H.3    Seino, S.4    Bell, G.I.5
  • 3
    • 0017184389 scopus 로고
    • A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
    • Bradford, M.M. 1976. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
    • (1976) Anal. Biochem. , vol.72 , pp. 248-254
    • Bradford, M.M.1
  • 5
    • 0027403163 scopus 로고
    • A Coomassie brilliant blue G250-based colorimetric assay for measuring activity of calpain and other proteases
    • Buroker-Kilgore, M. and K.K.W. Wang. 1993. A Coomassie brilliant blue G250-based colorimetric assay for measuring activity of calpain and other proteases. Anal. Biochem. 205:387-392.
    • (1993) Anal. Biochem. , vol.205 , pp. 387-392
    • Buroker-Kilgore, M.1    Wang, K.K.W.2
  • 6
    • 0027172007 scopus 로고
    • Detection of iron binding proteins by a blotting technique
    • Chen, Y. and J. Drysdale. 1993. Detection of iron binding proteins by a blotting technique. Anal. Biochem. 212:47-49.
    • (1993) Anal. Biochem. , vol.212 , pp. 47-49
    • Chen, Y.1    Drysdale, J.2
  • 7
    • 0025973304 scopus 로고
    • Processing of the precursor of protamine P2 in mouse. Identification of intermediate by their insolubility in the presence of sodium dodecyl sulfate
    • Elsevier, S.M., J. Noiran and D. Carre-Eusebe. 1991. Processing of the precursor of protamine P2 in mouse. Identification of intermediate by their insolubility in the presence of sodium dodecyl sulfate. Eur. J. Biochem. 196:167-175.
    • (1991) Eur. J. Biochem. , vol.196 , pp. 167-175
    • Elsevier, S.M.1    Noiran, J.2    Carre-Eusebe, D.3
  • 8
    • 0001232852 scopus 로고
    • One-dimensional polyacrylamide gel electrophoresis
    • B.D. Hames and D. Rickwood (Eds.), IRL Press, Oxford
    • Hames, B.D. 1990. One-dimensional polyacrylamide gel electrophoresis, p. 1-148. In B.D. Hames and D. Rickwood (Eds.), Gel Electrophoresis of Proteins. A Practical Approach, 2nd ed. IRL Press, Oxford.
    • (1990) Gel Electrophoresis of Proteins. A Practical Approach, 2nd Ed. , pp. 1-148
    • Hames, B.D.1
  • 10
    • 0024415306 scopus 로고
    • Abnormality in prostacyclin-stimulatory activity in sera from diabetics
    • Inoguchi, T, F. Umeda, H. Ono, M. Kunisaki, J. Watanabe and H. Nawata. 1989. Abnormality in prostacyclin-stimulatory activity in sera from diabetics. Metabolism 38:837-842.
    • (1989) Metabolism , vol.38 , pp. 837-842
    • Inoguchi, T.1    Umeda, F.2    Ono, H.3    Kunisaki, M.4    Watanabe, J.5    Nawata, H.6
  • 11
    • 0027337406 scopus 로고
    • Detection of P210bcr-abl in mature granulocytes from Ph1-positive chronic myelogenous leukemia patients by an immunoblotting method
    • Kuwao, F. and I. Takahashi. 1993. Detection of P210bcr-abl in mature granulocytes from Ph1-positive chronic myelogenous leukemia patients by an immunoblotting method. Leukemia 7:1168-1173.
    • (1993) Leukemia , vol.7 , pp. 1168-1173
    • Kuwao, F.1    Takahashi, I.2
  • 12
    • 0014949207 scopus 로고
    • Cleavage of structural proteins during the assembly of the head of bacteriophage T4
    • Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
    • (1970) Nature , vol.227 , pp. 680-685
    • Laemmli, U.K.1
  • 13
    • 0026722530 scopus 로고
    • The interferon-inducible 15-kDa ubiquitin homolog conjugates to intracellular proteins
    • Loeb, K.R. and A.L. Haas. 1992. The interferon-inducible 15-kDa ubiquitin homolog conjugates to intracellular proteins. J. Biol. Chem. 267:7806-7813.
    • (1992) J. Biol. Chem. , vol.267 , pp. 7806-7813
    • Loeb, K.R.1    Haas, A.L.2
  • 15
    • 0012019183 scopus 로고
    • Hypoxic neuronal injury in tissue culture is associated with delayed calcium accumulation
    • Marcoux, F.W., A.W. Probert and M.L. Weber. 1990. Hypoxic neuronal injury in tissue culture is associated with delayed calcium accumulation. Stroke 21 (III Suppl.):71-74.
    • (1990) Stroke , vol.21 , Issue.3 SUPPL. , pp. 71-74
    • Marcoux, F.W.1    Probert, A.W.2    Weber, M.L.3
  • 16
    • 0024566494 scopus 로고
    • Quantification of fibronectin in extracts of human aortae by an ELISA
    • Rasmussen, L.M. and L. Helckendrof. 1989. Quantification of fibronectin in extracts of human aortae by an ELISA. Scand. J. Clin. Lab. Invest. 49:205-210.
    • (1989) Scand. J. Clin. Lab. Invest. , vol.49 , pp. 205-210
    • Rasmussen, L.M.1    Helckendrof, L.2
  • 17
    • 0026454458 scopus 로고
    • Positive regulation of μ-calpain action by polyphosphoinositides
    • Saido, T.C., M. Shibata, T. Takenawa, H. Murofushi and K. Suzuki. 1992. Positive regulation of μ-calpain action by polyphosphoinositides. J. Biol. Chem. 267:24585-24590.
    • (1992) J. Biol. Chem. , vol.267 , pp. 24585-24590
    • Saido, T.C.1    Shibata, M.2    Takenawa, T.3    Murofushi, H.4    Suzuki, K.5
  • 18
    • 0009482260 scopus 로고
    • Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications
    • Towbin, H., T. Staehelin and J. Gordon. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76:4350-4354.
    • (1979) Proc. Natl. Acad. Sci. USA , vol.76 , pp. 4350-4354
    • Towbin, H.1    Staehelin, T.2    Gordon, J.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.